| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,249,681 | A→C | 26.5% | F111V (TTC→GTC) | malE ← | maltose transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,249,681 | 0 | A | C | 26.5% | 46.2 / 44.7 | 53 | F111V (TTC→GTC) | malE | maltose transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (20/19); new base C (12/2); total (32/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.90e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01 | |||||||||||
GGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGCCACCAAAGCGGTCGTGTGCC > W3110S.gb/4249615‑4249748 | ggTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCt < 1:959021/71‑1 (MQ=255) aaTCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCg < 1:2142020/71‑1 (MQ=255) aGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGACCGCTTTGTCCGGGGt > 1:3103303/1‑71 (MQ=255) aGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGACCGCTTTGTCCGGGGt > 1:559385/1‑71 (MQ=255) aGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGACCGCTTTGTCCGGGGt > 1:374749/1‑71 (MQ=255) aGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGt > 1:3119807/1‑71 (MQ=255) aGCTTGCCGT‑‑TAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCttt > 1:3807693/1‑60 (MQ=255) tGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGACCGCTTTGTCCGGGGTGAtt > 1:3347291/1‑71 (MQ=255) gCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGAttt > 1:2347723/1‑71 (MQ=255) gCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGAtt > 1:1671246/1‑70 (MQ=255) ccGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGTGGTGAttt < 1:3677469/70‑1 (MQ=255) ccGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGAttt < 1:1222542/70‑1 (MQ=255) ccGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGAttt < 1:518940/70‑1 (MQ=255) ccGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGAttt < 1:3392349/70‑1 (MQ=255) gTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTc > 1:1916080/1‑54 (MQ=255) gTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGcc < 1:330888/70‑1 (MQ=255) cGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGACCGCTTTGTcc > 1:1192843/1‑47 (MQ=255) cGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGACCGCTTTGTcc > 1:1188922/1‑47 (MQ=255) cGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGACCGCTTTGTcc > 1:1010898/1‑47 (MQ=255) cGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGACCGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCa < 1:2801271/71‑1 (MQ=255) cGGAATCCCAGGTAAACGGATACAGCTTGTCCTGGACCGCTTTGTcc > 1:478506/1‑47 (MQ=255) gCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAAc < 1:2800025/63‑1 (MQ=255) ccAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTc > 1:1918802/1‑39 (MQ=255) ccAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTc > 1:2395201/1‑39 (MQ=255) ccAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTc > 1:3179535/1‑39 (MQ=255) ggTAAACGGATACAGCTTGTCCTGGACCGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCg > 1:539496/1‑70 (MQ=255) gTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGAt < 1:974546/44‑1 (MQ=255) gTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTa < 1:2094964/71‑1 (MQ=255) aaaCGGATACAGCTTGTCCTGGACCGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGc > 1:3396900/1‑66 (MQ=255) aaaCGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGc > 1:3336936/1‑71 (MQ=255) aaaCGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGc > 1:2146913/1‑71 (MQ=255) aaCGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTTATTTCAGCCAACAGGCCAGATTGAGCGTAGc < 1:3160392/70‑1 (MQ=255) aaCGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGc < 1:3416127/55‑1 (MQ=255) cGGATACAGCTTGTCCTGGACCGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGcca > 1:3617620/1‑70 (MQ=255) cGGATACAGCTTGTCCTGGACCGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGcca > 1:1548415/1‑70 (MQ=255) cGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCa > 1:3817250/1‑55 (MQ=255) cGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGa < 1:801303/61‑1 (MQ=255) gATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGGGTAGccacca < 1:640551/71‑1 (MQ=255) gATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGccacca < 1:3438281/71‑1 (MQ=255) gATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGccacca < 1:3973846/71‑1 (MQ=255) tACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTGAGCCAACAGGCCAg < 1:3652333/52‑1 (MQ=255) cAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTa > 1:1484973/1‑60 (MQ=255) gTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAgg > 1:1788575/1‑40 (MQ=255) gTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGCCACCAAAGCGGTCgt > 1:1020177/1‑71 (MQ=255) tCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGCCACCAAAGCGGTCgtg < 1:2907796/71‑1 (MQ=255) cTGGACCGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGCCACCAAAGCGGTCgtgt < 1:1700889/70‑1 (MQ=255) cTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGCCACCAAAGCGGTCgtgt < 1:3809920/70‑1 (MQ=255) ggAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGa > 1:1343334/1‑40 (MQ=255) ggAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGa > 1:3031131/1‑44 (MQ=255) ggAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGa > 1:1311647/1‑44 (MQ=255) ggAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGa > 1:2299765/1‑44 (MQ=255) ggAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGa > 1:1024301/1‑44 (MQ=255) ggAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGCCACCAAAGCGGTCGTGTGcc > 1:2264369/1‑71 (MQ=255) | GGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGCCACCAAAGCGGTCGTGTGCC > W3110S.gb/4249615‑4249748 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |