| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,354,061 | C→T | 24.6% | G218G (GGG→GGA) | dcuR ← | DNA‑binding response regulator in two‑component regulatory system with DcuS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,354,061 | 0 | C | T | 24.6% | 114.0 / 35.2 | 61 | G218G (GGG→GGA) | dcuR | DNA‑binding response regulator in two‑component regulatory system with DcuS |
| Reads supporting (aligned to +/- strand): ref base C (20/26); new base T (7/8); total (27/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTA > W3110S.gb/4353997‑4354127 | aTTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGt < 1:2402788/70‑1 (MQ=255)aTTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGt < 1:2804789/70‑1 (MQ=255) tGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGTCCCGTGa < 1:663008/70‑1 (MQ=255) tGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGTCCCGTGAc > 1:750925/1‑71 (MQ=255) tatTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGt < 1:4012506/62‑1 (MQ=255) atTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATaa > 1:1082426/1‑71 (MQ=255) atTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATaa > 1:1012847/1‑71 (MQ=255) atTGTTACAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCAtga > 1:524997/1‑69 (MQ=255) ttGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAAt > 1:1587165/1‑71 (MQ=255) gTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATg > 1:3745922/1‑70 (MQ=255) tttCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGTCCCGTGACGCCATAATGGa < 1:3971928/71‑1 (MQ=255) ttCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGa < 1:3970384/70‑1 (MQ=255) tCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGAt < 1:4052377/70‑1 (MQ=255) agtagtgagtagtgTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACt < 1:740287/71‑1 (MQ=255) gtgagtagtgTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGt > 1:2544896/1‑70 (MQ=255) gtgagtagtgTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGt > 1:2253625/1‑70 (MQ=255) tgagtagtgTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCAt < 1:2974124/56‑1 (MQ=255) tgagtagtgTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCAt < 1:1862307/56‑1 (MQ=255) agtagtgTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGaa > 1:2072656/1‑70 (MQ=255) tagtgTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACaa < 1:2583058/71‑1 (MQ=255) gtgtTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAg < 1:66804/70‑1 (MQ=255) tgtTCTGCCTGAATACGGTAAGGGTAAACTGGACGCCCCGTGACGCCAt < 1:2111961/49‑1 (MQ=255) ttCTGCCTGAATACGGTAACTGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGAtgt > 1:1587494/1‑71 (MQ=255) ttCTGCCTGAATACGGTAACGGTAAACTGGACGTCCCGTGACGCCATAATGGATACTGGTGAACAAGa > 1:425130/1‑68 (MQ=255) ttCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACaa < 1:1304211/66‑1 (MQ=255) ttCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGAtgt > 1:3030864/1‑71 (MQ=255) atCTGCCTGAATACGGTAACGGTAAACTGGACGTCCCGTGACGCCATAATGGATACTGGTGAACAAGa > 1:1119529/2‑68 (MQ=255) cTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATa > 1:1773974/1‑53 (MQ=255) cTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATa > 1:2823538/1‑53 (MQ=255) cTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATa > 1:443453/1‑53 (MQ=255) tGCCTGAATACGGTAACGGTAAACTGGACGCCCCGt < 1:1870927/36‑1 (MQ=255) tGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCAt < 1:599014/44‑1 (MQ=255) gCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGAAGCCATAATGGATACTGGTGAACAAGATGTgg > 1:3434368/1‑69 (MQ=255) cTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATg < 1:426098/45‑1 (MQ=255) taaTACGGTAACGGTAAACTGGACGCCCCGTGACGCCAt < 1:2271770/38‑1 (MQ=255) gAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGa > 1:543522/1‑45 (MQ=255) aaTACGGTAACGGTAAACTGGACGTCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGtt < 1:619085/69‑1 (MQ=255) aaTACGGTAACGGTAAACTGGACGCCCCGTGACGCCAt < 1:1179409/38‑1 (MQ=255) aTACGGTAACGGTAAACTGGACGTCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGa < 1:3737938/70‑1 (MQ=255) aTACGGTAACGGTAAACTGGACGTCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGAc < 1:1114119/71‑1 (MQ=255) tACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAg < 1:618192/56‑1 (MQ=255) tACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAg < 1:581810/56‑1 (MQ=255) cggtaacggtaaACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGAccag > 1:385942/1‑71 (MQ=255) ggtaacggtaaACTGGACGTCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGAccagc > 1:3174016/1‑71 (MQ=255) ggtaacggtaaACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGAccagc < 1:3433783/71‑1 (MQ=255) gtaacggtaaACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGAccagc < 1:1078931/70‑1 (MQ=255) gtaacggtaaACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGAccagc > 1:1358491/1‑70 (MQ=255) gtaacggtaaACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGAccagc > 1:1363297/1‑70 (MQ=255) taacggtaaACTGGACGTCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGAccagcca < 1:3997180/71‑1 (MQ=255) aacggtaaACTGGACGCCCCGTGACGCCATAATGGa > 1:4077479/1‑36 (MQ=255) acggtaaACTGGACGTCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGAc > 1:2536742/1‑63 (MQ=255) ggtaaACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGAt > 1:1514323/1‑70 (MQ=255) gtaaACTGGACGTCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGAc < 1:2570200/60‑1 (MQ=255) aaaCTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGAtg < 1:1364988/47‑1 (MQ=255) aCTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTa < 1:2431071/71‑1 (MQ=255) gACGTCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTAtt < 1:1178893/69‑1 (MQ=255) gACGTCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTa > 1:3163715/1‑71 (MQ=255) gACGTCACGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTa > 1:3417910/1‑71 (MQ=255) gACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTAtt < 1:414579/69‑1 (MQ=255) gACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTAtt < 1:4008872/69‑1 (MQ=255) gACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTAtt < 1:2102045/69‑1 (MQ=255) | ATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTA > W3110S.gb/4353997‑4354127 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |