Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	4,361,585	C→T	36.1%	P571P (CCG→CCA)	cadA ←	lysine decarboxylase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,361,585	0	C	T	36.1%	61.9 / 60.0	61	P571P (CCG→CCA)	cadA	lysine decarboxylase 1
Reads supporting (aligned to +/- strand):  ref base C (20/19);  new base T (15/7);  total (35/26)
Fisher's exact test for biased strand distribution p-value = 2.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTGGATATTCTGAGCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAAGTCAGTCAGAGCACGCAGC  >  W3110S.gb/4361515‑4361651                                                                       |                                                                   tgtgGATATTCTGAGCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGAc                                                                    >  1:1571425/1‑71 (MQ=255) tgtgGATATTCTGAGCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGAc                                                                    >  1:3779241/1‑71 (MQ=255)     gATATTCTGAGCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGgc                                                                 <  1:309940/70‑1 (MQ=255)     gATATTCTGAGCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGgc                                                                 <  1:1313553/70‑1 (MQ=255)         ttCTGAGCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCAt                                                            >  1:948913/1‑71 (MQ=255)         ttCTGAGCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCAt                                                            >  1:2300255/1‑71 (MQ=255)         ttCTGAGCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCAt                                                            >  1:3523201/1‑71 (MQ=255)           cTGAGCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGt                                                          >  1:1627243/1‑71 (MQ=255)           cTGAGCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGt                                                          >  1:2531575/1‑71 (MQ=255)           cTGAGCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGt                                                          >  1:2440828/1‑71 (MQ=255)               gCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGttttt                                                      <  1:2330718/71‑1 (MQ=255)                   gTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGttttt                                                      <  1:3482558/67‑1 (MQ=255)                     tCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTcaca                                                  >  1:1308604/1‑69 (MQ=255)                     tCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTcaca                                                  >  1:456850/1‑69 (MQ=255)                     tCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTcaca                                                  >  1:1922184/1‑69 (MQ=255)                       cTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCa                                              <  1:1099440/71‑1 (MQ=255)                         gAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGTTTTTCACACGCAgg                                            >  1:1436381/1‑71 (MQ=255)                         gAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGTTTTTCACACGCAgg                                            >  1:3459888/1‑71 (MQ=255)                           aTACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCACCATGTTTTTCACACGCAGGtt                                          <  1:623450/71‑1 (MQ=255)                           aTACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGtt                                          <  1:1318771/71‑1 (MQ=255)                              cGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGttt                                                        >  1:2922158/1‑54 (MQ=255)                              cGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGttt                                                        >  1:1257545/1‑54 (MQ=255)                              cGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAg                                       >  1:328014/1‑71 (MQ=255)                                  tGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGTTTTTCACACGCAGGTTc                                         <  1:812670/65‑1 (MQ=255)                                  tGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGTTTTTCACACGCAGGTTc                                         <  1:2379909/65‑1 (MQ=255)                                  tGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGTTTTTCACACGCAGGTTCAGGTCg                                   <  1:529608/71‑1 (MQ=255)                                  tGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCTGCATGTTTTTCACACGCAGGTTCAGGTc                                    >  1:789636/1‑70 (MQ=255)                                  tGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCg                                   <  1:1133659/71‑1 (MQ=255)                                   gTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCa                                              <  1:3206713/59‑1 (MQ=255)                                    ttttCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGTTTTTCACACGCAGGtt                                          >  1:1564450/1‑62 (MQ=255)                                    ttttCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGaa                                 >  1:3925679/1‑71 (MQ=255)                                    ttttCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCa                                              >  1:3945492/1‑58 (MQ=255)                                      ttCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACg                               >  1:233359/1‑71 (MQ=255)                                      ttCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACg                               >  1:3966879/1‑71 (MQ=255)                                      ttCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACg                               >  1:879678/1‑71 (MQ=255)                                        cATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACg                               <  1:3628056/69‑1 (MQ=255)                                        cATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACg                               <  1:1765098/69‑1 (MQ=255)                                        cATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGc                              <  1:1172023/70‑1 (MQ=255)                                         aTAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCTAACGCa                             <  1:3050210/70‑1 (MQ=255)                                         aTAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCa                             <  1:1923356/70‑1 (MQ=255)                                         aTAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCa                             <  1:1928050/70‑1 (MQ=255)                                           aGAATTCAGGATCTTCACGATACAGAGATGgcagc                                                              >  1:2096479/1‑35 (MQ=255)                                            gAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGtt                         >  1:1540119/1‑71 (MQ=255)                                              aTTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGttt                        >  1:2605350/1‑70 (MQ=255)                                                 cAGGATCTTCACGATACAGAGATGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAag                    >  1:3530921/1‑71 (MQ=255)                                                 cAGGATCTTCACGATACAGAGATGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAag                    >  1:3962618/1‑71 (MQ=255)                                                    gATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCa                             <  1:2810845/59‑1 (MQ=255)                                                     aTCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCa                             <  1:1613800/58‑1 (MQ=255)                                                      tCTTCACGATACAGAGATGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAagtcagt               >  1:375122/1‑71 (MQ=255)                                                       cTTCACGATACAGAGATGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAagtcagt               >  1:1333954/1‑70 (MQ=255)                                                       cTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAagtcagt               >  1:2204628/1‑70 (MQ=255)                                                       cTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAagtcagt               >  1:81278/1‑70 (MQ=255)                                                       cTTCACGATACACAGATGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACg                           >  1:3583445/1‑58 (MQ=255)                                                         tCACGATACAGAGATGGCAGCATGTTTTTCACACGCAGGTTc                                         <  1:1357988/42‑1 (MQ=255)                                                           aCGATACAGAGATGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAAGTCAGTCagag          <  1:389299/71‑1 (MQ=255)                                                           aCGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAAGTCAGTCagag          <  1:442226/71‑1 (MQ=255)                                                             gATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTaaa                     >  1:925612/1‑58 (MQ=255)                                                               tACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCg                                   <  1:3534510/42‑1 (MQ=255)                                                                 cAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAATGTCAGTCAGAGCACg      >  1:4009265/1‑69 (MQ=255)                                                                 cAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAAGTCAGTCAGAGCACg      >  1:1473840/1‑69 (MQ=255)                                                                 cAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAAAGCACGTTTAAAGTCAGTCAGAGCACg      >  1:3330248/1‑69 (MQ=255)                                                                   gagaCGGCTGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAAGTCAGTCAGAGCACgcagc  <  1:2231975/71‑1 (MQ=255)                                                                   gagaCGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAAGTCAGTCAGAGCACgcagc  <  1:3193104/71‑1 (MQ=255)                                                                       |                                                                   TGTGGATATTCTGAGCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAAGTCAGTCAGAGCACGCAGC  >  W3110S.gb/4361515‑4361651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑