| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,405,345 | G→A | 27.6% | intergenic (+67/‑9) | hfq → / → hflX | HF‑I, host factor for RNA phage Q beta replication/predicted GTPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,405,345 | 0 | G | A | 27.6% | 43.4 / 17.4 | 29 | intergenic (+67/‑9) | hfq/hflX | HF‑I, host factor for RNA phage Q beta replication/predicted GTPase |
| Reads supporting (aligned to +/- strand): ref base G (13/8); new base A (5/3); total (18/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 | |||||||||||
TAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCGGTACTGGTACACATCTATTTTAC > W3110S.gb/4405276‑4405409 | tAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGt > 1:1727305/1‑71 (MQ=255) aGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTta < 1:561954/71‑1 (MQ=255) ttCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACg > 1:3784107/1‑71 (MQ=255) tttttACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGa > 1:2629187/1‑69 (MQ=255) tttttACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGAcc > 1:1670664/1‑71 (MQ=255) ccAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGt > 1:1549671/1‑70 (MQ=255) ccAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGt > 1:2056693/1‑70 (MQ=255) gCGATCCTGCGTTACCCGCTGATCTATTTAGAGGATTATACg > 1:1762902/1‑42 (MQ=37) ccGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTta < 1:2654785/37‑1 (MQ=255) cGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCa < 1:3277295/71‑1 (MQ=255) gATCCTGCGTTCCCCGCTGATCTATTTAGAGGATTATACGCTTGTTTGACCGTTATGATGCTGGt > 1:3833096/1‑65 (MQ=255) gATCCTGCGTTCCCCGCTGATCTATTTAGAGGATTATACGCTTGTTTGACCGTTATGATGCTGGt > 1:2068188/1‑65 (MQ=255) aTCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGt < 1:852725/52‑1 (MQ=255) ccTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTat > 1:3540830/1‑53 (MQ=255) gCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTatga > 1:347618/1‑52 (MQ=255) gCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTatga > 1:1083124/1‑52 (MQ=255) cGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCGGTAc < 1:15278/71‑1 (MQ=255) ttCCCCGCTGATCTATTTAGAGGATTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCGGTACt < 1:48067/70‑1 (MQ=255) ccccGCTGATCTATTTAGAGGATTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCg > 1:4064915/1‑63 (MQ=255) ccccGCTGATCTATTTAGAGGATTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCg > 1:4069565/1‑63 (MQ=255) cccGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCGGTACTGGTa < 1:2030964/71‑1 (MQ=255) cccGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCGGTACTGGTa < 1:2756057/71‑1 (MQ=255) gCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGt < 1:1723407/38‑1 (MQ=255) tGATCTATTTAGAGGATTATACGCTTGTTTGACCGTTATGATGCTGGTGAg < 1:1893449/51‑1 (MQ=255) aTCTATTTAGAGGATTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCGGTACTgg < 1:3271305/62‑1 (MQ=255) tCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCGGTACTGGTACACATCTa > 1:2455391/1‑71 (MQ=255) cTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCGGTACTGGTACACATCTAt > 1:2092452/1‑71 (MQ=255) cTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCGGTACTGGTACACATCTAt > 1:1880485/1‑71 (MQ=255) tAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCGGTACTGGTACACATCTATTTTAc > 1:2714213/1‑71 (MQ=255) | TAAGGTTTCGGGCTGTTTTTTTACACGGGGAGCCAGCGATCCTGCGTTCCCCGCTGATCTATTTAGAGGGTTATACGCTTGTTTGACCGTTATGATGCTGGTGAGCAGGCGGTACTGGTACACATCTATTTTAC > W3110S.gb/4405276‑4405409 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |