| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 342,943 | T→C | 47.1% | V279A (GTT→GCT) | yahK → | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 342,943 | 0 | T | C | 47.1% | ‑0.9 / 46.8 | 34 | V279A (GTT→GCT) | yahK | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (5/13); new base C (6/10); total (11/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGAAACTCAGGA > W3110S.gb/342876‑343012 | ttGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGctt > 1:2196283/1‑70 (MQ=255)ttGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGctt > 1:2015202/1‑70 (MQ=255) tGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGtttt > 1:3217427/1‑70 (MQ=255) tGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGtttt > 1:926387/1‑70 (MQ=255) tGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGcttt > 1:1279413/1‑70 (MQ=255) gCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTc > 1:2880696/1‑70 (MQ=255) gTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGtttt > 1:3329509/1‑61 (MQ=255) tGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGCTTTCAACCTGATCa > 1:931665/1‑71 (MQ=255) tGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGa < 1:1824918/71‑1 (MQ=255) tGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGa < 1:3559446/71‑1 (MQ=255) tGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGCTTTCAACCTGATCATGa < 1:2761493/71‑1 (MQ=255) ccATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGa < 1:2255063/66‑1 (MQ=255) ccATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGa < 1:2167708/66‑1 (MQ=255) tGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGtt < 1:2011325/47‑1 (MQ=255) tGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCt < 1:3798409/55‑1 (MQ=255) aCGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGc < 1:3535047/71‑1 (MQ=255) aCGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGc < 1:1596688/71‑1 (MQ=255) cGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCg < 1:9264/71‑1 (MQ=255) gCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGa < 1:1630167/71‑1 (MQ=255) gCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGa < 1:3974055/71‑1 (MQ=255) cTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTTATCATg < 1:714/44‑1 (MQ=255) cTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCa > 1:375164/1‑42 (MQ=255) cTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCa > 1:2728287/1‑42 (MQ=255) cTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATg < 1:1578829/44‑1 (MQ=255) cTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATg < 1:1005612/44‑1 (MQ=255) cTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATg < 1:1098674/71‑1 (MQ=255) tGCGACACCGCATAAATCGCCGGAAGCTTTCAACCTGATCATg < 1:1512923/43‑1 (MQ=255) tGCGACACCGCATAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGa > 1:246131/1‑71 (MQ=255) acCGCATAAATCGCCGGAAGTTTTAAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGAt > 1:2026514/1‑66 (MQ=255) cGCATAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGa > 1:2663557/1‑48 (MQ=255) cGCATAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGa > 1:3909504/1‑48 (MQ=255) cGCATAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTggcggc < 1:1252525/71‑1 (MQ=255) gCATAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCa > 1:3462437/1‑71 (MQ=255) gCATAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCa > 1:1873640/1‑71 (MQ=255) tAAATCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGc < 1:2474617/42‑1 (MQ=255) aaTCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTc > 1:1152301/1‑69 (MQ=255) tCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGa < 1:3078977/71‑1 (MQ=255) tCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTc < 1:1815170/50‑1 (MQ=255) tCGCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTc < 1:107141/50‑1 (MQ=255) gCCGGAAGCTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGaa < 1:19339/70‑1 (MQ=255) gTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGAAACTCAGga < 1:697246/71‑1 (MQ=255) ttttCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGAAACTc < 1:2294193/66‑1 (MQ=255) ttttCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGAAACTc < 1:3686626/66‑1 (MQ=255) | TTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTCAACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGAAACTCAGGA > W3110S.gb/342876‑343012 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |