| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,445,264 | C→T | 10.3% | V212I (GTT→ATT) | ytfL ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,445,264 | 0 | C | T | 10.3% | 169.4 / 8.9 | 58 | V212I (GTT→ATT) | ytfL | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (17/35); new base T (2/4); total (19/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
AGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGC > W3110S.gb/4445197‑4445334 | aGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGt < 1:2042596/71‑1 (MQ=255) ggCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGt < 1:1404895/70‑1 (MQ=255) cTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACCCGGTGTCATTGAAGACGGAACGGTACg > 1:691137/1‑71 (MQ=255) ttGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACgg > 1:343222/1‑70 (MQ=255) tGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCTGTGTCATTGAAGACGGAACGGTACgg < 1:460899/69‑1 (MQ=255) tGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACgg < 1:2095111/69‑1 (MQ=255) tGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACgg < 1:637817/69‑1 (MQ=255) tCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTc > 1:3462325/1‑71 (MQ=255) aTCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTcc < 1:2588671/70‑1 (MQ=255) tCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACgg < 1:3963497/63‑1 (MQ=255) cTTCGAGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGc > 1:3381457/1‑71 (MQ=255) ttCGTGGAGTTCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGc > 1:1415901/1‑70 (MQ=255) ttCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCt > 1:170574/1‑71 (MQ=255) tGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCaaa < 1:2771462/71‑1 (MQ=255) cAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGttt > 1:1571462/1‑71 (MQ=255) cAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGtt > 1:2423073/1‑70 (MQ=255) aaaCCAAATCACGTTTTCACGCTGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGtttt < 1:1615413/71‑1 (MQ=255) aaCCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTc < 1:4041589/71‑1 (MQ=255) aCCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCa < 1:369646/71‑1 (MQ=255) aCCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCa < 1:2052841/71‑1 (MQ=255) ccAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCaa < 1:1246816/71‑1 (MQ=255) cAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAATGGTACGGGa > 1:2962459/1‑47 (MQ=255) cAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAATGGTACGGGa > 1:3546342/1‑47 (MQ=255) cAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACgg < 1:1280665/45‑1 (MQ=255) cAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAAt > 1:450413/1‑71 (MQ=255) cAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAAt < 1:2785541/71‑1 (MQ=255) cAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAAt < 1:1664242/71‑1 (MQ=255) cAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAAt < 1:194155/71‑1 (MQ=255) aaaTCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACgg < 1:3841428/44‑1 (MQ=255) aaaTCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACgg < 1:1616408/44‑1 (MQ=255) aTCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGa > 1:557011/1‑44 (MQ=255) aTCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGa > 1:4029854/1‑44 (MQ=255) aTCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGa > 1:786080/1‑44 (MQ=255) tCACGTTTTCACGCGGTGTCATTGAAGACGGAATGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGc < 1:1356972/71‑1 (MQ=255) tCACGTTTTCACGCGGTGTCATTGAAGACGGAATGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGc < 1:2743275/71‑1 (MQ=255) tCACGTTTTCACGCGGTGTCATTGAAGACGGAATGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGc < 1:2779121/71‑1 (MQ=255) tCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACgg < 1:637366/41‑1 (MQ=255) gtCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACg > 1:1106037/1‑71 (MQ=255) gtCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACg > 1:1312677/1‑71 (MQ=255) tCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACg > 1:3788099/1‑70 (MQ=255) tCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGt < 1:3319718/71‑1 (MQ=255) tCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGt < 1:2538246/71‑1 (MQ=255) cATTGAAGACGGAATGGTACGGGATTCCAGCTCAAAGACGTTTTc < 1:2666534/45‑1 (MQ=255) aTTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAAt > 1:2282863/1‑47 (MQ=255) aTTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGt < 1:302238/69‑1 (MQ=255) aTTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGt < 1:1780306/69‑1 (MQ=255) aTTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGt < 1:1840903/69‑1 (MQ=255) aTTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTaa < 1:61424/71‑1 (MQ=255) aaGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAacac < 1:2210361/70‑1 (MQ=255) aaGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAacac < 1:1247220/70‑1 (MQ=255) aaGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAacac < 1:4012083/70‑1 (MQ=255) ggAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTTTTTACGt < 1:1014578/60‑1 (MQ=255) ggAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGt < 1:2139107/60‑1 (MQ=255) ggAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGt < 1:75053/60‑1 (MQ=255) ggAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACAcgcccgc < 1:2654816/71‑1 (MQ=255) ggAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCATGTTTACGt < 1:2367480/60‑1 (MQ=255) gAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAacac < 1:1260732/64‑1 (MQ=255) aaCGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAg > 1:684332/1‑39 (MQ=255) cGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAgc > 1:3374084/1‑71 (MQ=255) | AGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGC > W3110S.gb/4445197‑4445334 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |