Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,445,264 C→T 10.3% V212I (GTT→ATT)  ytfL ← predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,445,2640CT10.3% 169.4 / 8.9 58V212I (GTT→ATT) ytfLpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (17/35);  new base T (2/4);  total (19/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

AGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGC  >  W3110S.gb/4445197‑4445334
                                                                   |                                                                      
aGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGt                                                                     <  1:2042596/71‑1 (MQ=255)
 ggCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGt                                                                     <  1:1404895/70‑1 (MQ=255)
   cTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACCCGGTGTCATTGAAGACGGAACGGTACg                                                                  >  1:691137/1‑71 (MQ=255)
     ttGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACgg                                                                 >  1:343222/1‑70 (MQ=255)
      tGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCTGTGTCATTGAAGACGGAACGGTACgg                                                                 <  1:460899/69‑1 (MQ=255)
      tGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACgg                                                                 <  1:2095111/69‑1 (MQ=255)
      tGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACgg                                                                 <  1:637817/69‑1 (MQ=255)
         tCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTc                                                            >  1:3462325/1‑71 (MQ=255)
           aTCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTcc                                                           <  1:2588671/70‑1 (MQ=255)
            tCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACgg                                                                 <  1:3963497/63‑1 (MQ=255)
             cTTCGAGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGc                                                        >  1:3381457/1‑71 (MQ=255)
              ttCGTGGAGTTCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGc                                                        >  1:1415901/1‑70 (MQ=255)
              ttCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCt                                                       >  1:170574/1‑71 (MQ=255)
                  tGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCaaa                                                   <  1:2771462/71‑1 (MQ=255)
                         cAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGttt                                            >  1:1571462/1‑71 (MQ=255)
                         cAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGtt                                             >  1:2423073/1‑70 (MQ=255)
                          aaaCCAAATCACGTTTTCACGCTGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGtttt                                           <  1:1615413/71‑1 (MQ=255)
                           aaCCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTc                                          <  1:4041589/71‑1 (MQ=255)
                            aCCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCa                                         <  1:369646/71‑1 (MQ=255)
                            aCCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCa                                         <  1:2052841/71‑1 (MQ=255)
                             ccAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCaa                                        <  1:1246816/71‑1 (MQ=255)
                              cAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAATGGTACGGGa                                                               >  1:2962459/1‑47 (MQ=255)
                              cAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAATGGTACGGGa                                                               >  1:3546342/1‑47 (MQ=255)
                              cAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACgg                                                                 <  1:1280665/45‑1 (MQ=255)
                              cAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAAt                                       >  1:450413/1‑71 (MQ=255)
                              cAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAAt                                       <  1:2785541/71‑1 (MQ=255)
                              cAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAAt                                       <  1:1664242/71‑1 (MQ=255)
                              cAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAAt                                       <  1:194155/71‑1 (MQ=255)
                               aaaTCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACgg                                                                 <  1:3841428/44‑1 (MQ=255)
                               aaaTCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACgg                                                                 <  1:1616408/44‑1 (MQ=255)
                                 aTCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGa                                                               >  1:557011/1‑44 (MQ=255)
                                 aTCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGa                                                               >  1:4029854/1‑44 (MQ=255)
                                 aTCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGa                                                               >  1:786080/1‑44 (MQ=255)
                                  tCACGTTTTCACGCGGTGTCATTGAAGACGGAATGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGc                                   <  1:1356972/71‑1 (MQ=255)
                                  tCACGTTTTCACGCGGTGTCATTGAAGACGGAATGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGc                                   <  1:2743275/71‑1 (MQ=255)
                                  tCACGTTTTCACGCGGTGTCATTGAAGACGGAATGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGc                                   <  1:2779121/71‑1 (MQ=255)
                                  tCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACgg                                                                 <  1:637366/41‑1 (MQ=255)
                                                   gtCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACg                  >  1:1106037/1‑71 (MQ=255)
                                                   gtCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACg                  >  1:1312677/1‑71 (MQ=255)
                                                    tCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACg                  >  1:3788099/1‑70 (MQ=255)
                                                    tCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGt                 <  1:3319718/71‑1 (MQ=255)
                                                    tCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGt                 <  1:2538246/71‑1 (MQ=255)
                                                     cATTGAAGACGGAATGGTACGGGATTCCAGCTCAAAGACGTTTTc                                          <  1:2666534/45‑1 (MQ=255)
                                                      aTTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAAt                                       >  1:2282863/1‑47 (MQ=255)
                                                      aTTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGt                 <  1:302238/69‑1 (MQ=255)
                                                      aTTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGt                 <  1:1780306/69‑1 (MQ=255)
                                                      aTTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGt                 <  1:1840903/69‑1 (MQ=255)
                                                      aTTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTaa               <  1:61424/71‑1 (MQ=255)
                                                          aaGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAacac            <  1:2210361/70‑1 (MQ=255)
                                                          aaGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAacac            <  1:1247220/70‑1 (MQ=255)
                                                          aaGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAacac            <  1:4012083/70‑1 (MQ=255)
                                                               ggAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTTTTTACGt                 <  1:1014578/60‑1 (MQ=255)
                                                               ggAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGt                 <  1:2139107/60‑1 (MQ=255)
                                                               ggAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGt                 <  1:75053/60‑1 (MQ=255)
                                                               ggAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACAcgcccgc      <  1:2654816/71‑1 (MQ=255)
                                                               ggAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCATGTTTACGt                 <  1:2367480/60‑1 (MQ=255)
                                                                gAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAacac            <  1:1260732/64‑1 (MQ=255)
                                                                 aaCGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAg                                    >  1:684332/1‑39 (MQ=255)
                                                                   cGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAgc  >  1:3374084/1‑71 (MQ=255)
                                                                   |                                                                      
AGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTACGTAACACGCCCGCCAGC  >  W3110S.gb/4445197‑4445334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: