| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,464,055 | A→T | 25.0% | intergenic (+62/‑180) | pmbA → / → cybC | predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17/ECK4231:JW4195:b4236; cytochrome b562, truncated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,464,055 | 0 | A | T | 25.0% | 105.8 / 40.0 | 60 | intergenic (+62/‑180) | pmbA/cybC | predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17/ECK4231:JW4195:b4236; cytochrome b562, truncated |
| Reads supporting (aligned to +/- strand): ref base A (26/19); new base T (9/6); total (35/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01 | |||||||||||
AAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAG > W3110S.gb/4463992‑4464123 | aaaaaaaCTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGAc > 1:2213020/1‑71 (MQ=255) aaaaaaCTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACg > 1:369641/1‑71 (MQ=255) gcgcGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTg < 1:3174909/69‑1 (MQ=255) gcgcGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTg < 1:157788/69‑1 (MQ=255) gcgcGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTg < 1:3297742/69‑1 (MQ=255) gcgcGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTg < 1:33129/69‑1 (MQ=255) cgACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAAt > 1:1821577/1‑69 (MQ=255) cgACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAAt > 1:4051346/1‑69 (MQ=255) ccTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCga < 1:2255143/52‑1 (MQ=255) cTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGa < 1:2687178/63‑1 (MQ=255) gCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGa > 1:3485289/1‑61 (MQ=255) gcgcTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCgggg < 1:3588189/71‑1 (MQ=255) gcTATAAACTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACGGAAAg > 1:2415497/1‑53 (MQ=255) gcTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAg < 1:2437494/71‑1 (MQ=255) cTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTg < 1:606900/54‑1 (MQ=255) cTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTg < 1:897289/54‑1 (MQ=255) cTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTg < 1:3440253/54‑1 (MQ=255) ataaaataaaAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAggg > 1:3049661/1‑60 (MQ=255) ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCgaga > 1:2720589/1‑43 (MQ=255) ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAg > 1:282117/1‑58 (MQ=255) ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAg > 1:3046939/1‑58 (MQ=255) ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAg > 1:3658847/1‑58 (MQ=255) ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAg > 1:701354/1‑58 (MQ=255) ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCg > 1:2567370/1‑70 (MQ=255) ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCg > 1:2909777/1‑70 (MQ=255) taAACTAAAAAATTACACAAAATCATTCGCACTGCTTc < 1:1504320/38‑1 (MQ=255) taAACTAAAAAATTACACAAAATCATTCGCACTGCTTc < 1:1489694/38‑1 (MQ=255) taAACTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTg < 1:1893739/51‑1 (MQ=255) taAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCg < 1:2261040/69‑1 (MQ=255) aaCTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATg > 1:1138494/1‑53 (MQ=255) aaCTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACt > 1:4039332/1‑71 (MQ=255) aaCTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACt > 1:748230/1‑71 (MQ=255) aaCTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGaaa > 1:2893918/1‑46 (MQ=255) aaCTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGaaa > 1:2560824/1‑46 (MQ=255) aaCTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCg < 1:938310/67‑1 (MQ=255) aaCTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCg < 1:2239805/67‑1 (MQ=255) cTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAg > 1:968424/1‑45 (MQ=255) cTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAg > 1:2128206/1‑45 (MQ=255) cTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCg > 1:1517579/1‑71 (MQ=255) cTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCg > 1:537974/1‑71 (MQ=255) cTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGCAAAGTGAATGAGGGCCGGGGAGCGTACTCg > 1:3090922/1‑71 (MQ=255) aaaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGaa > 1:3244599/1‑47 (MQ=255) aaaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGaa > 1:1564449/1‑47 (MQ=255) aaaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGa > 1:1970842/1‑46 (MQ=255) aaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGaa < 1:1117179/40‑1 (MQ=255) aaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGaa < 1:1845397/40‑1 (MQ=255) aaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGaa < 1:3464146/40‑1 (MQ=255) aaaaaTTACACAAAATCATTAGCACTGCATCGAGACGGaa < 1:3354295/40‑1 (MQ=255) aaTTACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTAc > 1:965252/1‑71 (MQ=255) aTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACg > 1:2084106/1‑71 (MQ=255) ttACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGt < 1:3911050/71‑1 (MQ=255) ttACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGt > 1:3723892/1‑71 (MQ=255) ttACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGt > 1:2673864/1‑71 (MQ=255) ttACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGt < 1:1530298/71‑1 (MQ=255) ttACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGt > 1:2834764/1‑71 (MQ=255) tACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGt > 1:2122799/1‑70 (MQ=255) acacAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCg < 1:2434753/61‑1 (MQ=255) tGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGAccc > 1:442106/1‑56 (MQ=255) gCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGc < 1:632652/43‑1 (MQ=255) cATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAg > 1:2312653/1‑70 (MQ=255) | AAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAG > W3110S.gb/4463992‑4464123 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |