| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,482,473 | A→G | 35.5% | L174P (CTC→CCC) | argI ← | ornithine carbamoyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,482,473 | 0 | A | G | 35.5% | 57.0 / 62.5 | 62 | L174P (CTC→CCC) | argI | ornithine carbamoyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base A (15/25); new base G (6/16); total (21/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01 | |||||||||||
CTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTT > W3110S.gb/4482408‑4482540 | cTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATc < 1:3586380/71‑1 (MQ=255)cTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATc < 1:3027827/71‑1 (MQ=255) tCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGGGCATCg > 1:1441111/1‑70 (MQ=255) tCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCg > 1:1777656/1‑70 (MQ=255) ccGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCTAGCATCg < 1:2008648/69‑1 (MQ=255) ccGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGGGCATCg < 1:359229/69‑1 (MQ=255) ccGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGa < 1:3221224/70‑1 (MQ=255) ccGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGa < 1:559358/70‑1 (MQ=255) ggCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAAtt < 1:3909205/71‑1 (MQ=255) catcACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGcc < 1:3315014/68‑1 (MQ=255) cAGCACGCTTGTGGCGCGACCAGCCGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAAt > 1:2170953/1‑67 (MQ=255) cAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGaa > 1:3113928/1‑66 (MQ=255) cAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGcc > 1:2397515/1‑71 (MQ=255) cAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGcc > 1:3133086/1‑71 (MQ=255) aGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGGGCATCGa < 1:1020091/64‑1 (MQ=255) gCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCg < 1:2357074/62‑1 (MQ=255) gCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCg < 1:533882/62‑1 (MQ=255) aCGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGGGCATCGAATTGCCCAtg < 1:3722393/71‑1 (MQ=255) cGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgt < 1:805743/71‑1 (MQ=255) cTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgttg > 1:3558747/1‑71 (MQ=255) cTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgttg > 1:1358597/1‑71 (MQ=255) cTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgttg > 1:1121607/1‑71 (MQ=255) gtgGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGGGCATCGAATTGCCCAtgttgt < 1:1419693/69‑1 (MQ=255) gtgGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGGGCATCGAATTGCCCAtgttgt < 1:1940976/69‑1 (MQ=255) gtgGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGGGCATCGAATTGCCCAtgttgt < 1:933068/69‑1 (MQ=255) gtgGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgttgt < 1:2360019/69‑1 (MQ=255) gtgGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgttgt < 1:2007480/69‑1 (MQ=255) gtgGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgttgt < 1:1846635/69‑1 (MQ=255) tgGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGGGCATCGAATTGCCCAtgttgt < 1:4005819/68‑1 (MQ=255) gACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGt < 1:1275004/71‑1 (MQ=255) gACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGt < 1:3206813/71‑1 (MQ=255) cAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCAc > 1:3738018/1‑71 (MQ=255) cAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCAc > 1:1703809/1‑71 (MQ=255) cAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCAc > 1:3055417/1‑71 (MQ=255) aGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCAcc > 1:2443880/1‑71 (MQ=255) aCGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGGGCATCGAATTGCCCATgttgtt < 1:1875266/57‑1 (MQ=255) aCGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGGGCATCGAATTGCCCATgttgtt < 1:185672/57‑1 (MQ=255) aCGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGGGCATCGAATTGCCCATgttgtt < 1:1039576/57‑1 (MQ=255) aCGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCg < 1:3959890/41‑1 (MQ=255) aCGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTg < 1:2800042/71‑1 (MQ=255) aCGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTg < 1:1045391/71‑1 (MQ=255) aCGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTg < 1:1868877/71‑1 (MQ=255) cGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCAc > 1:1021120/1‑67 (MQ=255) gCAAATCCAGACCGGTAAGCGCCGCAGCTTCGGGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa < 1:3860745/71‑1 (MQ=255) gCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGc > 1:2854748/1‑45 (MQ=255) gCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa < 1:606048/71‑1 (MQ=255) cAAATCCAGACCGGTAAGCGCCGCAGCTTCGGGCATCGa < 1:917782/39‑1 (MQ=255) cAAATCCAGACCGGTAAGCGCCGCAGCTTCGGGCATCGa < 1:222683/39‑1 (MQ=255) cAAATCCAGACCGGTAAGCGCCGCAGCTTCGGGCATCGa < 1:2098247/39‑1 (MQ=255) cAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTa > 1:3985616/1‑49 (MQ=255) cGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTc < 1:1602788/71‑1 (MQ=255) aGCGCCGCAGCTTCGGGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTc < 1:1199393/71‑1 (MQ=255) aGCGCCGCAGCTTCGGGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGGCATTTc < 1:2798611/71‑1 (MQ=255) gcgcCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGAc < 1:2046356/58‑1 (MQ=255) gcgcCGCAGCTTCGAGCATCGAATTGCCCATGTTCTTACGCGCGTCACCTGCATAGAc < 1:846129/58‑1 (MQ=255) ccgcAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTg < 1:149647/71‑1 (MQ=255) gcAGCTTCGGGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATa > 1:3192031/1‑50 (MQ=255) gcAGCTTCGGGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATa > 1:3436776/1‑50 (MQ=255) gcAGCTTCGGGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATa > 1:3160001/1‑50 (MQ=255) tCGGGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCAttt > 1:1604469/1‑58 (MQ=255) tCGGGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCttt > 1:720538/1‑71 (MQ=255) gAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATa < 1:42649/42‑1 (MQ=255) | CTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTT > W3110S.gb/4482408‑4482540 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |