Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	4,487,895	T→C	21.4%	K209E (AAA→GAA)	valS ←	valyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,487,895	0	T	C	21.4%	63.7 / 14.5	34	K209E (AAA→GAA)	valS	valyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (14/12);  new base C (2/5);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGATTCGCGGTTTTCGACTTCCAG  >  W3110S.gb/4487830‑4487964                                                                  |                                                                      aTCGCCCAGCAGGGTTTCTTTACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCg                                                                     <  1:3995806/68‑1 (MQ=255)  tCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCAcc                                                                 <  1:2768884/71‑1 (MQ=255)  tCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCAcc                                                                 >  1:3233939/1‑71 (MQ=255)  tCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCAcc                                                                 >  1:3798846/1‑71 (MQ=255)  tCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCAcc                                                                 >  1:3805335/1‑71 (MQ=255)  tCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACAAGATAATCTTTACCGTCTGCGGTTTACGCa                                                                   >  1:2575791/1‑69 (MQ=255)    gCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGt                                                               >  1:297980/1‑71 (MQ=255)           agGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTcagccag                                                        <  1:547382/71‑1 (MQ=255)           agGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTcagccag                                                        <  1:2647450/71‑1 (MQ=255)             ggTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTCCGCACCGTCAGCCagcg                                                      <  1:2273470/71‑1 (MQ=255)                      aCGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCagcggatagcgga                                             <  1:2393032/71‑1 (MQ=255)                      aCGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTCCGCACCGTCAGCCagcggatagcgga                                             <  1:670717/71‑1 (MQ=255)                                gcgACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACAt                                   >  1:3412710/1‑71 (MQ=255)                                gcgACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACAt                                   >  1:3892977/1‑71 (MQ=255)                                gcgACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACAt                                   >  1:1837181/1‑71 (MQ=255)                                   accaccAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGa                                <  1:2554648/71‑1 (MQ=255)                                   accaccAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGa                                <  1:2096142/71‑1 (MQ=255)                                   accaccAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGa                                <  1:1321940/71‑1 (MQ=255)                                         aGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCagcg                                                      <  1:335979/43‑1 (MQ=255)                                          gATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTc                         >  1:3856069/1‑71 (MQ=255)                                            tAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGa                       >  1:1439521/1‑71 (MQ=255)                                             aaTCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGAt                      >  1:2958/1‑71 (MQ=255)                                             aaTCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGAt                      >  1:1638602/1‑71 (MQ=255)                                             aaTCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGAt                      >  1:3144657/1‑71 (MQ=255)                                              aTCTTTACCGTCTGCGGTTTCCGCACCGTCAGCCagcg                                                      <  1:2309330/38‑1 (MQ=255)                                              aTCTTTACCGTCTGCGGTTTCCGCACCGTCAGCCagcg                                                      <  1:219171/38‑1 (MQ=255)                                              aTCTTTACCGTCTGCGGTTTCCGCACCGTCAGCCagcg                                                      <  1:1801744/38‑1 (MQ=255)                                                   tACCGTCTGCGGTTTCCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGaa                               >  1:3730792/1‑56 (MQ=255)                                                   tACCGTCTGCGGTTTCCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGATTCGCgg                >  1:1979343/1‑71 (MQ=255)                                                        tCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGATTCGCgg                <  1:3063094/66‑1 (MQ=255)                                                        tCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGATTCGCGGTTTTc           <  1:2626641/71‑1 (MQ=255)                                                        tCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGATTCGCGGTTTTc           <  1:2505510/71‑1 (MQ=255)                                                          tGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGaa                               >  1:2561531/1‑49 (MQ=255)                                                          tGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGaa                               >  1:2812598/1‑49 (MQ=255)                                                                 ttCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGATTCGCGGTTTTCGACTTCCAg  >  1:2693874/1‑71 (MQ=255)                                                                 ttCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGATTCGCGGTTTTCGACTTCCAg  >  1:2688952/1‑71 (MQ=255)                                                                 ttCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGATTCGCGGTTTTCGACTTCCAg  >  1:3649357/1‑71 (MQ=255)                                                                 ttCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGATTCGCGGTTTTCGACTTCCAg  >  1:2536670/1‑71 (MQ=255)                                                                 ttCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGATTCGCGGTTTTCGACTTCCAg  >  1:561183/1‑71 (MQ=255)                                                                  |                                                                      ATCGCCCAGCAGGGTTTCTGGACGGGTAGTCGCGACCACCAGATAATCTTTACCGTCTGCGGTTTTCGCACCGTCAGCCAGCGGATAGCGGATGTGCCACATCGAACCTTTCGATTCGCGGTTTTCGACTTCCAG  >  W3110S.gb/4487830‑4487964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑