| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,511,217 | T→C | 8.3% | intergenic (+130/+91) | yjhC → / ← yjhD | predicted oxidoreductase/ECK4271:JW5770:b4281; KpLE2 phage‑like element; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,511,217 | 0 | T | C | 8.3% | 190.0 / 9.1 | 72 | intergenic (+130/+91) | yjhC/yjhD | predicted oxidoreductase/ECK4271:JW5770:b4281; KpLE2 phage‑like element; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (27/39); new base C (3/3); total (30/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
CATAATTTTAGCAGTGCCATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGCTACGTAATACGGGATAGTTACGA > W3110S.gb/4511159‑4511285 | cATAATTTTAGCAGTGCCATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCtgtg > 1:2297743/1‑71 (MQ=255) aTAATTTTAGCAGTGTCATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCtgtg > 1:1732470/1‑70 (MQ=255) aTAATTTTAGCAGTGCCATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCtgtg > 1:823398/1‑70 (MQ=255) aTAATTTTAGCAGTGCCATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCtgtg > 1:1118575/1‑70 (MQ=255) aaTTTTAGCAGTGCCATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTAt > 1:665513/1‑71 (MQ=255) aaTTTTAGCAGTGCCATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTAt > 1:1346682/1‑71 (MQ=255) ttttAGCAGTGCCATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTAttt < 1:2184233/71‑1 (MQ=255) gTGCCATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCa < 1:2521545/71‑1 (MQ=255) gTGCCATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCa < 1:738596/71‑1 (MQ=255) gTGCCATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCa < 1:2541674/71‑1 (MQ=255) gTGCCATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCa < 1:1416882/71‑1 (MQ=255) gTGCCATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCa < 1:118636/71‑1 (MQ=255) tGCCATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCa < 1:516795/70‑1 (MQ=255) ccATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTcccc > 1:616883/1‑67 (MQ=255) ccATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCatat > 1:2820326/1‑71 (MQ=255) ccATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCatat > 1:1733452/1‑71 (MQ=255) ccATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTCTCTTCCTCTGTGTATTTGTTCCCCatat < 1:3175502/71‑1 (MQ=255) ccATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTCTCTTCCTCTGTGTATTTGTTCCCCatat < 1:65015/71‑1 (MQ=255) cATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATg < 1:1587151/71‑1 (MQ=255) cATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATg < 1:1889086/71‑1 (MQ=255) aTTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTTTGTATTTGTTCCCCATATg < 1:1191042/70‑1 (MQ=255) tttATGATAAAAACATCCCTTAAAACCAATCTAATCTTTttct < 1:120808/43‑1 (MQ=255) tttATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTgtgt < 1:16882/53‑1 (MQ=255) tATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCtg < 1:1692348/48‑1 (MQ=255) tATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTAt > 1:2987922/1‑53 (MQ=255) tATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTAt > 1:2367880/1‑53 (MQ=255) aTAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCAt < 1:1721635/70‑1 (MQ=255) aaaaCATCCCTTAAAACGAATCTAATCTTTCTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAAtt > 1:3567569/1‑71 (MQ=255) aaaaCATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCata > 1:3872982/1‑58 (MQ=255) aaaaCATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAAtt < 1:2378216/71‑1 (MQ=255) aaaaCATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAAtt < 1:709196/71‑1 (MQ=255) aaaaCATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAAtt < 1:3191996/71‑1 (MQ=255) aaaCATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCata > 1:478996/1‑57 (MQ=255) aaaCATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCata > 1:3192435/1‑57 (MQ=255) aaaCATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAAttt < 1:416487/71‑1 (MQ=255) aaaCATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAAttt < 1:773906/71‑1 (MQ=255) aaaCATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAAttt < 1:3742926/71‑1 (MQ=255) aaCATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAAtt < 1:2887801/69‑1 (MQ=255) aaCATCCCTTAAAACCAATCTAATCTTTCTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTa > 1:2225386/1‑71 (MQ=255) cATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTAc < 1:1793983/70‑1 (MQ=255) cATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTAc < 1:1663868/70‑1 (MQ=255) aTCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGc < 1:144190/71‑1 (MQ=255) aTCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGc < 1:2469485/71‑1 (MQ=255) aTCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGc < 1:3380197/71‑1 (MQ=255) aTCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGc > 1:3891843/1‑71 (MQ=255) tCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGCt < 1:1089506/71‑1 (MQ=255) cTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCa < 1:4040106/47‑1 (MQ=255) cTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCa < 1:2037746/47‑1 (MQ=255) ttAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGCTACg > 1:37387/1‑70 (MQ=255) aaaaCAAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCACATATGGCGTCATAATTTACg > 1:1613710/1‑63 (MQ=255) aaaCCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGCTACGTaa < 1:2493580/70‑1 (MQ=255) aaaCCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGCTACGTAAt < 1:2437558/71‑1 (MQ=255) aaaCCAATCTAATCTTTCTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGCTACGTaa < 1:62337/70‑1 (MQ=255) aCCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGCTACGTAATAc > 1:3422690/1‑71 (MQ=255) ccAATCAAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGCTACGTAATACg > 1:521305/1‑71 (MQ=255) cAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTccc < 1:529375/37‑1 (MQ=255) cAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGCTACGTAATACgg < 1:411298/71‑1 (MQ=255) cAATCTAATCTTTCTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGCTACGTAATACgg > 1:2767986/1‑71 (MQ=255) atctaatctTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAAtt < 1:1657439/52‑1 (MQ=255) tctaatctTTTTCTTCCTCTGTGTATTTGTTCCCCat < 1:1123688/37‑1 (MQ=255) tctaatctTTTTCTTCCTCTGTGTATTTGTTCCCCat < 1:1104618/37‑1 (MQ=255) tctaatctTTTTCTTCCTCTGTGTATTTGTTCCCCat < 1:278859/37‑1 (MQ=255) ctaatctTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTa > 1:1513624/1‑52 (MQ=255) ctaatctTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGCTACGTAATACGGGa > 1:3715785/1‑69 (MQ=255) ctaatctTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGCTACGTAATACGGGa > 1:2045883/1‑69 (MQ=255) taatctTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAAt > 1:3602165/1‑48 (MQ=255) taatctTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTa < 1:3109625/51‑1 (MQ=255) taatctTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTa < 1:802217/51‑1 (MQ=255) taatctTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTa < 1:1885031/51‑1 (MQ=255) taatctTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGCTACGTAATAc > 1:349192/1‑64 (MQ=255) taatctTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGCTACGTAATACGGGATAg > 1:3209868/1‑71 (MQ=255) taatctTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGCTACGTAATACGGGATAg > 1:1186258/1‑71 (MQ=255) atctTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTATGCTACGTAATACGGGATAGt > 1:209534/1‑70 (MQ=255) tttttCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAAtt > 1:1871145/1‑44 (MQ=255) tttttCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGCTACGTAATACGGGATAGTTAc > 1:2712979/1‑70 (MQ=255) ttttCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTa > 1:1500199/1‑45 (MQ=255) tttCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGCTACGTAATACGGGATAGTTAc < 1:2387975/68‑1 (MQ=255) tttCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGCTACGTAATACGGGATAGTTAc < 1:1176443/68‑1 (MQ=255) tttCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGCTACGTAATACGGGATAGTTAc < 1:2778189/68‑1 (MQ=255) tttCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGCTACGTAATACGGGATAGTTACGa < 1:14049/70‑1 (MQ=255) | CATAATTTTAGCAGTGCCATTTATGATAAAAACATCCCTTAAAACCAATCTAATCTTTTTCTTCCTCTGTGTATTTGTTCCCCATATGGCGTCATAATTTACGCTACGTAATACGGGATAGTTACGA > W3110S.gb/4511159‑4511285 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |