Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,525,729 A→G 6.0% V325A (GTT→GCT)  yjhF ← predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,525,7290AG 6.0% 183.1 / 3.5 66V325A (GTT→GCT) yjhFpredicted transporter
Reads supporting (aligned to +/- strand):  ref base A (26/36);  new base G (2/2);  total (28/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

CAGGATCGGGTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCCGCGATAGCTTTTCC  >  W3110S.gb/4525659‑4525796
                                                                      |                                                                   
cAGGATCGGGTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGaa                                                                     >  1:598581/1‑71 (MQ=255)
    aTCGGGTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAActtg                                                                 <  1:822941/71‑1 (MQ=255)
    aTCGGGTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAActtg                                                                 <  1:3747088/71‑1 (MQ=255)
       gggTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAActtgctt                                                              >  1:1064231/1‑71 (MQ=255)
        ggTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAGcttgcttg                                                             <  1:1073166/71‑1 (MQ=255)
        ggTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAGcttgcttg                                                             <  1:3351831/71‑1 (MQ=255)
        ggTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAActtgcttg                                                             <  1:2416250/71‑1 (MQ=255)
        ggTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAActtgcttg                                                             <  1:3743780/71‑1 (MQ=255)
         gTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGa                                                            <  1:3930211/71‑1 (MQ=255)
                tCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAActtgct                                                               >  1:3367187/1‑61 (MQ=255)
                tCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAActtgct                                                               >  1:2557133/1‑61 (MQ=255)
                tCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcac                                                      >  1:2057239/1‑70 (MQ=255)
                  cATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcaccac                                                   >  1:1788485/1‑71 (MQ=255)
                  cATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcaccac                                                   >  1:2802590/1‑71 (MQ=255)
                   aTACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcaccacc                                                  <  1:4079199/71‑1 (MQ=255)
                      cccGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcaccaccac                                                >  1:3292370/1‑70 (MQ=255)
                      cccGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcaccaccac                                                >  1:1348256/1‑70 (MQ=255)
                       ccGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAAc                                                                    <  1:26266/49‑1 (MQ=255)
                             ccAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcaccacc                                                  <  1:3810438/61‑1 (MQ=255)
                             ccAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATa                                        <  1:828103/71‑1 (MQ=255)
                             ccAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATa                                        <  1:3629320/71‑1 (MQ=255)
                               aGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACATGCTTGAATGcaccaccac                                                >  1:685005/1‑61 (MQ=255)
                                   tGGAAATATACTGACCAACGCCAGAGTCGATGAGAActtgcttg                                                             <  1:3851585/44‑1 (MQ=255)
                                    ggAAATATACTGACCAACGCCAGAGTCGATGAGAActtgcttg                                                             <  1:2920406/43‑1 (MQ=255)
                                     gAAATATACTGACCAACGCCAGAGTCGATGAGAGCTTGCTTGAATGCACCACCACCACCGATAATcagcag                                >  1:3498227/1‑71 (MQ=255)
                                     gAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGAt                                         <  1:3043694/62‑1 (MQ=255)
                                     gAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGAt                                         <  1:3716370/62‑1 (MQ=255)
                                     gAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATcagcag                                <  1:510770/71‑1 (MQ=255)
                                      aaaTATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcaccacc                                                  <  1:2549980/52‑1 (MQ=255)
                                         tataCTGACCAACGCCAGAGTCGATGAGAActtgcttg                                                             <  1:3397779/38‑1 (MQ=255)
                                         tataCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATcagcagcag                             <  1:2065015/70‑1 (MQ=255)
                                          ataCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcacca                                                    >  1:1202766/1‑46 (MQ=255)
                                          ataCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGAc                           >  1:239562/1‑71 (MQ=255)
                                           taCTGACCAACGCCAGAGTCGATGAGAGCTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGAcc                          >  1:2131968/1‑71 (MQ=255)
                                           taCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGaa                                                           <  1:1070983/38‑1 (MQ=255)
                                           taCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGaa                                                           <  1:4036934/38‑1 (MQ=255)
                                           taCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCCCCGATAATCAGCAGCAGAc                           >  1:3094253/1‑70 (MQ=255)
                                           taCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGAcc                          >  1:2719043/1‑71 (MQ=255)
                                           taCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGAcc                          >  1:982203/1‑71 (MQ=255)
                                           taCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGAc                           >  1:2492099/1‑70 (MQ=255)
                                            aCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATg                                                         <  1:566171/39‑1 (MQ=255)
                                             cTGACCAACGCCAGAGTCTATGAGAACTTGCTTGAATGCACCACCACCACCGATAATcagcag                                <  1:2717489/63‑1 (MQ=255)
                                             cTGACCAACGCCAGAGTCGATTAGAACTTGCTTGAATGCACCACCACCACCGATAATcagcag                                <  1:3768825/63‑1 (MQ=255)
                                             cTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATg                                                         >  1:2301490/1‑38 (MQ=255)
                                             cTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGc                                                        >  1:2769053/1‑39 (MQ=255)
                                             cTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATcagcag                                <  1:3927895/63‑1 (MQ=255)
                                             cTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCg                         >  1:1939512/1‑70 (MQ=255)
                                             cTGACCAACGCCACAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATcagcag                                <  1:4023786/63‑1 (MQ=255)
                                                aCCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGc                       <  1:805031/69‑1 (MQ=255)
                                                        cAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCcgcg             <  1:2061578/71‑1 (MQ=255)
                                                         agagTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCaa                     >  1:3118941/1‑62 (MQ=255)
                                                            gTCGATGAGAACTTGCTTGAATGCACCACCAGCACCGATAATCAGCAGCAGACCGGc                       <  1:3729559/57‑1 (MQ=255)
                                                            gTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGc                       <  1:807394/57‑1 (MQ=255)
                                                            gTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGc                       <  1:1675428/57‑1 (MQ=255)
                                                            gTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGc                       <  1:4047686/57‑1 (MQ=255)
                                                              cGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCaa                     >  1:1685230/1‑57 (MQ=255)
                                                              cGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCaa                     >  1:1361281/1‑57 (MQ=255)
                                                              cGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCaa                     >  1:154320/1‑57 (MQ=255)
                                                              cGATGAGAACTTGCTAGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCaa                     >  1:1308965/1‑57 (MQ=255)
                                                               gATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCCGCGATAGCtt      <  1:1907564/71‑1 (MQ=255)
                                                               gATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCCGCGATAGCtt      >  1:1276842/1‑71 (MQ=255)
                                                               gATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCCGCGATAGCtt      <  1:3850410/71‑1 (MQ=255)
                                                               gATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCCGCGATAGCtt      >  1:898032/1‑71 (MQ=255)
                                                                 tGAGAACTTGCTTGAATGCACCACCACCACCGATAATca                                    <  1:1583018/39‑1 (MQ=255)
                                                                 tGAGAACTTGCTTGAATGCACCACCACCACCGATAATca                                    <  1:1191336/39‑1 (MQ=255)
                                                                 tGAGAACTTGCTTGAATGCACCACCACCACCGATAATca                                    <  1:1096538/39‑1 (MQ=255)
                                                                   agaACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCCGCGATAGCTTTTcc  >  1:2112139/1‑71 (MQ=255)
                                                                     aaCTTGCTTGAATGCACCACCACCACCGATAATcagcagc                               >  1:1761943/1‑40 (MQ=255)
                                                                     aaCTTGATTGAATGCACCACCACCACCGATAATcagc                                  >  1:3547002/1‑37 (MQ=255)
                                                                      |                                                                   
CAGGATCGGGTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCCGCGATAGCTTTTCC  >  W3110S.gb/4525659‑4525796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: