| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,525,729 | A→G | 6.0% | V325A (GTT→GCT) | yjhF ← | predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,525,729 | 0 | A | G | 6.0% | 183.1 / 3.5 | 66 | V325A (GTT→GCT) | yjhF | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base A (26/36); new base G (2/2); total (28/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01 | |||||||||||
CAGGATCGGGTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCCGCGATAGCTTTTCC > W3110S.gb/4525659‑4525796 | cAGGATCGGGTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGaa > 1:598581/1‑71 (MQ=255) aTCGGGTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAActtg < 1:822941/71‑1 (MQ=255) aTCGGGTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAActtg < 1:3747088/71‑1 (MQ=255) gggTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAActtgctt > 1:1064231/1‑71 (MQ=255) ggTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAGcttgcttg < 1:1073166/71‑1 (MQ=255) ggTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAGcttgcttg < 1:3351831/71‑1 (MQ=255) ggTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAActtgcttg < 1:2416250/71‑1 (MQ=255) ggTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAActtgcttg < 1:3743780/71‑1 (MQ=255) gTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGa < 1:3930211/71‑1 (MQ=255) tCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAActtgct > 1:3367187/1‑61 (MQ=255) tCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAActtgct > 1:2557133/1‑61 (MQ=255) tCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcac > 1:2057239/1‑70 (MQ=255) cATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcaccac > 1:1788485/1‑71 (MQ=255) cATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcaccac > 1:2802590/1‑71 (MQ=255) aTACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcaccacc < 1:4079199/71‑1 (MQ=255) cccGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcaccaccac > 1:3292370/1‑70 (MQ=255) cccGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcaccaccac > 1:1348256/1‑70 (MQ=255) ccGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAAc < 1:26266/49‑1 (MQ=255) ccAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcaccacc < 1:3810438/61‑1 (MQ=255) ccAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATa < 1:828103/71‑1 (MQ=255) ccAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATa < 1:3629320/71‑1 (MQ=255) aGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACATGCTTGAATGcaccaccac > 1:685005/1‑61 (MQ=255) tGGAAATATACTGACCAACGCCAGAGTCGATGAGAActtgcttg < 1:3851585/44‑1 (MQ=255) ggAAATATACTGACCAACGCCAGAGTCGATGAGAActtgcttg < 1:2920406/43‑1 (MQ=255) gAAATATACTGACCAACGCCAGAGTCGATGAGAGCTTGCTTGAATGCACCACCACCACCGATAATcagcag > 1:3498227/1‑71 (MQ=255) gAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGAt < 1:3043694/62‑1 (MQ=255) gAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGAt < 1:3716370/62‑1 (MQ=255) gAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATcagcag < 1:510770/71‑1 (MQ=255) aaaTATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcaccacc < 1:2549980/52‑1 (MQ=255) tataCTGACCAACGCCAGAGTCGATGAGAActtgcttg < 1:3397779/38‑1 (MQ=255) tataCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATcagcagcag < 1:2065015/70‑1 (MQ=255) ataCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcacca > 1:1202766/1‑46 (MQ=255) ataCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGAc > 1:239562/1‑71 (MQ=255) taCTGACCAACGCCAGAGTCGATGAGAGCTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGAcc > 1:2131968/1‑71 (MQ=255) taCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGaa < 1:1070983/38‑1 (MQ=255) taCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGaa < 1:4036934/38‑1 (MQ=255) taCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCCCCGATAATCAGCAGCAGAc > 1:3094253/1‑70 (MQ=255) taCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGAcc > 1:2719043/1‑71 (MQ=255) taCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGAcc > 1:982203/1‑71 (MQ=255) taCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGAc > 1:2492099/1‑70 (MQ=255) aCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATg < 1:566171/39‑1 (MQ=255) cTGACCAACGCCAGAGTCTATGAGAACTTGCTTGAATGCACCACCACCACCGATAATcagcag < 1:2717489/63‑1 (MQ=255) cTGACCAACGCCAGAGTCGATTAGAACTTGCTTGAATGCACCACCACCACCGATAATcagcag < 1:3768825/63‑1 (MQ=255) cTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATg > 1:2301490/1‑38 (MQ=255) cTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGc > 1:2769053/1‑39 (MQ=255) cTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATcagcag < 1:3927895/63‑1 (MQ=255) cTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCg > 1:1939512/1‑70 (MQ=255) cTGACCAACGCCACAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATcagcag < 1:4023786/63‑1 (MQ=255) aCCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGc < 1:805031/69‑1 (MQ=255) cAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCcgcg < 1:2061578/71‑1 (MQ=255) agagTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCaa > 1:3118941/1‑62 (MQ=255) gTCGATGAGAACTTGCTTGAATGCACCACCAGCACCGATAATCAGCAGCAGACCGGc < 1:3729559/57‑1 (MQ=255) gTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGc < 1:807394/57‑1 (MQ=255) gTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGc < 1:1675428/57‑1 (MQ=255) gTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGc < 1:4047686/57‑1 (MQ=255) cGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCaa > 1:1685230/1‑57 (MQ=255) cGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCaa > 1:1361281/1‑57 (MQ=255) cGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCaa > 1:154320/1‑57 (MQ=255) cGATGAGAACTTGCTAGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCaa > 1:1308965/1‑57 (MQ=255) gATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCCGCGATAGCtt < 1:1907564/71‑1 (MQ=255) gATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCCGCGATAGCtt > 1:1276842/1‑71 (MQ=255) gATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCCGCGATAGCtt < 1:3850410/71‑1 (MQ=255) gATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCCGCGATAGCtt > 1:898032/1‑71 (MQ=255) tGAGAACTTGCTTGAATGCACCACCACCACCGATAATca < 1:1583018/39‑1 (MQ=255) tGAGAACTTGCTTGAATGCACCACCACCACCGATAATca < 1:1191336/39‑1 (MQ=255) tGAGAACTTGCTTGAATGCACCACCACCACCGATAATca < 1:1096538/39‑1 (MQ=255) agaACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCCGCGATAGCTTTTcc > 1:2112139/1‑71 (MQ=255) aaCTTGCTTGAATGCACCACCACCACCGATAATcagcagc > 1:1761943/1‑40 (MQ=255) aaCTTGATTGAATGCACCACCACCACCGATAATcagc > 1:3547002/1‑37 (MQ=255) | CAGGATCGGGTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCCGCGATAGCTTTTCC > W3110S.gb/4525659‑4525796 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |