| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 57,363 | T→C | 10.2% | intergenic (‑254/‑1) | imp ← / → djlA | exported protein required for envelope biosynthesis and integrity/DnaJ‑like protein, membrane anchored |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 57,363 | 0 | T | C | 10.2% | 156.5 / 9.5 | 59 | intergenic (‑254/‑1) | imp/djlA | exported protein required for envelope biosynthesis and integrity/DnaJ‑like protein, membrane anchored |
| Reads supporting (aligned to +/- strand): ref base T (30/23); new base C (3/3); total (33/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
AAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCTTT > W3110S.gb/57296‑57423 | aaaaCGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATg < 1:1873238/71‑1 (MQ=255) aCGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAg < 1:399178/71‑1 (MQ=255) aCGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAg < 1:3636459/71‑1 (MQ=255) cGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAg < 1:1281775/70‑1 (MQ=255) aaaGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAatat < 1:347897/59‑1 (MQ=255) aaaGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCa > 1:4022194/1‑64 (MQ=255) aaaGCACCTTTATATTGTGCGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAatat < 1:629872/59‑1 (MQ=255) tttATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGa < 1:1712908/66‑1 (MQ=255) tataTTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTggg > 1:3331658/1‑62 (MQ=255) tataTTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAAt < 1:4020952/69‑1 (MQ=255) tataTTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAAt < 1:1439848/69‑1 (MQ=255) tataTTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCa > 1:2456532/1‑71 (MQ=255) tataTTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCa > 1:3092848/1‑71 (MQ=255) tataTTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATACATGCAGTATTGGGGAAAAAt < 1:1143983/69‑1 (MQ=255) tataTTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATACATGCAGTATTGGGGAAAAAt < 1:1918685/69‑1 (MQ=255) tataTTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATACATGCAGTATTGGGGAAAAATCa > 1:3700373/1‑71 (MQ=255) tataTTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATACATGCAGTATTGGGGAAAAATCa > 1:2377356/1‑71 (MQ=255) atatTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCa < 1:1311380/70‑1 (MQ=255) tatTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCa < 1:2557594/69‑1 (MQ=255) ttGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTgg > 1:1905105/1‑71 (MQ=255) agaTAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGaaa < 1:728995/55‑1 (MQ=255) gaTAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGcgtggccg > 1:3326584/1‑71 (MQ=255) aTAGCCCTGATATCCGTGTGTCGATTTGGGGAATACATGCAGTATTGGGGAAAAATCa < 1:3346507/58‑1 (MQ=255) aGCCCTGATATCCGTGTGTCGATTTGGGGAATACATGCAGTATTGGGGAAAAATCATTGGcgtggccgtg > 1:2395712/1‑70 (MQ=255) gCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGcgtggccgtggc > 1:3000640/1‑71 (MQ=255) gCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGcgtggccgtggc > 1:1277951/1‑71 (MQ=255) gccgATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGGGGCCGTGGCCt < 1:1924017/68‑1 (MQ=255) ccTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCt < 1:2209194/71‑1 (MQ=255) cTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCtt < 1:2365613/71‑1 (MQ=255) tGATATCCGTGTGTCGATTTGGGGAATATATGCAg < 1:1483049/35‑1 (MQ=255) gATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTAc > 1:1118297/1‑71 (MQ=255) gATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTAc > 1:3476254/1‑71 (MQ=255) atatCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCa > 1:2561839/1‑49 (MQ=255) atatCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCa > 1:511078/1‑49 (MQ=255) atatCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCt < 1:3282980/67‑1 (MQ=255) atCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTa > 1:3829890/1‑67 (MQ=255) atCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTa > 1:3578105/1‑67 (MQ=255) atCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGa > 1:547217/1‑71 (MQ=255) tCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAAt < 1:1294284/44‑1 (MQ=255) tCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAAt < 1:3293522/44‑1 (MQ=255) tCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGcgtggccgt < 1:1986774/59‑1 (MQ=255) tCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTa > 1:1208191/1‑66 (MQ=255) tCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGAt > 1:2687686/1‑71 (MQ=255) ccGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCAtt > 1:3045318/1‑47 (MQ=255) ccGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCAtt > 1:1709695/1‑47 (MQ=255) ccGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCAtt > 1:3027325/1‑47 (MQ=255) ccGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCAtt > 1:138823/1‑47 (MQ=255) ccGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCAtt > 1:3839526/1‑47 (MQ=255) cGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATg < 1:3568291/70‑1 (MQ=255) gtgtgtCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGa > 1:3685416/1‑67 (MQ=255) gtgtgtCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGa > 1:2229330/1‑67 (MQ=255) tCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGggcggcg > 1:1283200/1‑71 (MQ=255) gATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGggcggcggc > 1:949004/1‑71 (MQ=255) gATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGggcggcggc > 1:3920504/1‑71 (MQ=255) gATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGggcggcggc > 1:3252109/1‑71 (MQ=255) gATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGggcggcgg > 1:2554778/1‑70 (MQ=255) gATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGggcggcg > 1:3078860/1‑69 (MQ=255) tttGGGGAATATATGCAGTATTGGGGAAAAATCATTGGcgtggcc < 1:1834545/45‑1 (MQ=255) tttGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCtt < 1:3776083/71‑1 (MQ=255) tGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCttt < 1:3412707/70‑1 (MQ=255) tGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCttt < 1:2994816/70‑1 (MQ=255) atatatGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGggcggcg > 1:1218646/1‑59 (MQ=255) tatatGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACt < 1:690002/47‑1 (MQ=255) | AAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCTTT > W3110S.gb/57296‑57423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |