| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 346,362 | C→T | 11.5% | E436K (GAA→AAA) | prpR ← | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 346,362 | 0 | C | T | 11.5% | 148.1 / 9.4 | 52 | E436K (GAA→AAA) | prpR | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (27/19); new base T (3/3); total (30/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GTCGTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCA > W3110S.gb/346296‑346430 | gTCGTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTc < 1:1900274/67‑1 (MQ=255)gTCGTTCCATCATATTGCGCAGTTAACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTc < 1:2924786/67‑1 (MQ=255) cGTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCAc > 1:1983412/1‑67 (MQ=255) gTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGcc > 1:910008/1‑71 (MQ=255) gTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGcc > 1:632715/1‑71 (MQ=255) gTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGcc > 1:1874/1‑71 (MQ=255) tcatATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTTACtt < 1:400868/62‑1 (MQ=255) tcatATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTTACtt < 1:3384084/62‑1 (MQ=255) tcatATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTTACtt < 1:1689212/62‑1 (MQ=255) atatTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTTACTTGc > 1:3069146/1‑62 (MQ=255) atatTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTTACTTGc > 1:62338/1‑62 (MQ=255) atatTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTTACTTGc > 1:2888530/1‑62 (MQ=255) ttGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTg > 1:2047204/1‑71 (MQ=255) gcAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGt < 1:2488659/71‑1 (MQ=255) ttCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTc < 1:273326/45‑1 (MQ=255) ttCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGt < 1:193403/67‑1 (MQ=255) cACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATg > 1:1518509/1‑69 (MQ=255) atatTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGa < 1:3272948/71‑1 (MQ=255) tatTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGt < 1:1379447/59‑1 (MQ=255) tatTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGaa < 1:2962895/71‑1 (MQ=255) tatTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGaa < 1:3424512/71‑1 (MQ=255) tatTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGaa < 1:2298767/71‑1 (MQ=255) tatTGCCTGGCCAGTCGTAGGGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGaa < 1:3044306/71‑1 (MQ=255) ttGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACtt > 1:3588722/1‑39 (MQ=255) ttGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACtt > 1:1503469/1‑39 (MQ=255) ttGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTaa > 1:2897870/1‑59 (MQ=255) ttGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTaa > 1:2954945/1‑59 (MQ=255) ttGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTaa > 1:3721258/1‑59 (MQ=255) ttGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATgcag > 1:106749/1‑64 (MQ=255) ttGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATgcag > 1:3104429/1‑64 (MQ=255) ttGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATgcag > 1:3333282/1‑64 (MQ=255) ttGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGaaaa > 1:620211/1‑71 (MQ=255) ccTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATgg < 1:3358839/71‑1 (MQ=255) gCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCg < 1:3526650/71‑1 (MQ=255) ccAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGaa < 1:319568/61‑1 (MQ=255) gTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAgcg < 1:722500/71‑1 (MQ=255) gTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAgcg < 1:529977/71‑1 (MQ=255) aGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATg < 1:1166777/45‑1 (MQ=255) cagcagcaCAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGcgccgc > 1:3448451/1‑69 (MQ=255) cagcagcaCAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGcgccgc > 1:1663760/1‑69 (MQ=255) cagcagcaCAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGcgccgc > 1:1929512/1‑69 (MQ=255) cagcagcaCAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGcgccgc > 1:3240099/1‑69 (MQ=255) cagcagcaCAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGcgccgc > 1:2358632/1‑69 (MQ=255) aagcagcaCAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGcgccgc > 1:4014739/2‑69 (MQ=255) gcaCAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGG‑AGAGAGCGCCGCCAGAGaca < 1:1674142/71‑1 (MQ=255) cacaGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGaca < 1:1827859/71‑1 (MQ=255) tttCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGaaa < 1:3391126/39‑1 (MQ=255) tttCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGaaa < 1:844945/39‑1 (MQ=255) tttCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGaaa < 1:528144/39‑1 (MQ=255) tCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAAt > 1:540182/1‑39 (MQ=255) tCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAAt > 1:2032200/1‑39 (MQ=255) tCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAAt > 1:1938352/1‑39 (MQ=255) tCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATggg > 1:3654887/1‑42 (MQ=255) tCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGagag > 1:3793490/1‑50 (MQ=255) tCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCa > 1:665408/1‑70 (MQ=255) | GTCGTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCA > W3110S.gb/346296‑346430 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |