| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,546,724 | T→C | 41.4% | S2S (AGT→AGC) | fimE → | tyrosine recombinase/inversion of on/off regulator of fimA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,546,724 | 0 | T | C | 41.4% | 55.2 / 115.1 | 96 | S2S (AGT→AGC) | fimE | tyrosine recombinase/inversion of on/off regulator of fimA |
| Reads supporting (aligned to +/- strand): ref base T (33/23); new base C (21/17); total (55/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGACCGATTGAGGTTTCCTATAGGTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTACGGGGCAAC > W3110S.gb/4546656‑4546789 | tGACCGATTGAGGTTTCCTATAGGTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGTa < 1:714085/70‑1 (MQ=255) ttGAGGTTTCCTATAGGTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGCaaa < 1:1269229/65‑1 (MQ=255) aGGTTTCCTATAGGTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTAt < 1:2217654/71‑1 (MQ=255) tttGCTATAGGTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGTa < 1:1258992/57‑1 (MQ=255) aGGTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTAc > 1:1888798/1‑65 (MQ=255) ggTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTa > 1:3308914/1‑69 (MQ=255) ggTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTa > 1:1235578/1‑69 (MQ=255) ggTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTa > 1:1655218/1‑69 (MQ=255) ggTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTa > 1:1960395/1‑69 (MQ=255) tATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTa < 1:3011053/67‑1 (MQ=255) tATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAaag < 1:1576535/70‑1 (MQ=255) attcattcAAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAaaga < 1:2816590/70‑1 (MQ=255) ttcattcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTaaa > 1:4016884/1‑67 (MQ=255) ttcattcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTaaa > 1:2351116/1‑67 (MQ=255) ttcattcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGACGTTATCTTACCGGTaaa > 1:2794978/1‑67 (MQ=255) cattcaAATATATCTTAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGtt > 1:3359115/1‑71 (MQ=255) cattcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCg > 1:2896452/1‑60 (MQ=255) cattcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCg > 1:2973433/1‑60 (MQ=255) cattcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGtt > 1:2953468/1‑71 (MQ=255) cattcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGtt > 1:3790469/1‑71 (MQ=255) cattcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGGAAACGTCGTTATCTTACCGGTAAAGAAGtt > 1:3983375/1‑71 (MQ=255) cattcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGCAAAcgt > 1:1865862/1‑47 (MQ=255) cattcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTa > 1:714224/1‑52 (MQ=255) cattcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTa > 1:3925068/1‑52 (MQ=255) cattcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTa > 1:2977801/1‑52 (MQ=255) cattcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGtt > 1:1914992/1‑71 (MQ=255) attcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGt < 1:36141/69‑1 (MQ=255) caAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAgg > 1:3706858/1‑71 (MQ=255) caAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAgg > 1:138844/1‑71 (MQ=255) caAATATATCTCAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAgg > 1:2343900/1‑71 (MQ=255) aaTATATCTCAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGcc < 1:599904/71‑1 (MQ=255) tatatCTCAGTTAGGAGTACTACTATTGTGAGTAAAcgtc < 1:3350691/40‑1 (MQ=255) tatatCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCat < 1:2724138/71‑1 (MQ=255) tatatCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCat < 1:3055633/71‑1 (MQ=255) tatatCTCAGTTAGGAGTACTACTATTGTGAGCaa > 1:85869/1‑35 (MQ=255) tatatCTCAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCat < 1:1314389/71‑1 (MQ=255) tatatCTCAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCat < 1:3977127/71‑1 (MQ=255) tatatCTCAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCat < 1:487131/71‑1 (MQ=255) tatatCTCAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCat < 1:789897/71‑1 (MQ=255) atatCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTc > 1:1743727/1‑63 (MQ=255) tctcAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTa < 1:3566087/51‑1 (MQ=255) tctcAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTa < 1:3055070/51‑1 (MQ=255) tctcAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCat < 1:3792907/67‑1 (MQ=255) tcAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCatgatg > 1:125694/1‑69 (MQ=255) cAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAg > 1:3915696/1‑71 (MQ=255) aGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCa < 1:1468939/69‑1 (MQ=255) ttAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCat < 1:35255/61‑1 (MQ=255) ttAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCat < 1:1319694/61‑1 (MQ=255) aGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCatga > 1:1836442/1‑61 (MQ=255) aGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCatga > 1:3096520/1‑61 (MQ=255) aGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCatga > 1:2628652/1‑61 (MQ=255) gAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCat < 1:2793453/57‑1 (MQ=255) gAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCat < 1:3353051/57‑1 (MQ=255) gAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCat < 1:2081393/57‑1 (MQ=255) gTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGt < 1:1195131/71‑1 (MQ=255) gTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGt < 1:2610392/71‑1 (MQ=255) tactacTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGc < 1:4079993/51‑1 (MQ=255) tactacTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCat < 1:3330043/54‑1 (MQ=255) tactacTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCat < 1:822051/54‑1 (MQ=255) tactacTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCat < 1:2713187/54‑1 (MQ=255) tactacTATTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGtt < 1:410391/71‑1 (MQ=255) ctactaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGtt > 1:3449755/1‑65 (MQ=255) ctactaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGtt > 1:2480630/1‑65 (MQ=255) ctactaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGt < 1:3629815/68‑1 (MQ=255) ctactaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTAc > 1:1897381/1‑71 (MQ=255) ctactaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTAc < 1:556975/71‑1 (MQ=255) ctactaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTAc > 1:4054604/1‑71 (MQ=255) ctactaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTAc > 1:3995292/1‑71 (MQ=255) ctactaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTAc > 1:3642082/1‑71 (MQ=255) ctactaTTGTGAGTAAACGTCGTTATCTTAACGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTAc > 1:2930030/1‑71 (MQ=255) ctactaTTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTAc > 1:1490804/1‑71 (MQ=255) tactaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCatgatg < 1:2275778/55‑1 (MQ=255) ctaTTGTGAGTAAACGTCGTTATCTTACCGGTAaagaag > 1:40531/1‑39 (MQ=255) ctaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGt > 1:1911689/1‑65 (MQ=255) ctaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGt > 1:566505/1‑65 (MQ=255) ctaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGt > 1:876253/1‑65 (MQ=255) ctaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTACggg > 1:788391/1‑71 (MQ=255) ctaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTACgg > 1:953857/1‑70 (MQ=255) ctaTTGTGAGCAAACGTCGTTATCTTACCGGTAaagaa > 1:3974962/1‑38 (MQ=255) ctaTTGTGAGCAAACGTCGTTATCTTACCGGTAaagaa > 1:676955/1‑38 (MQ=255) ctaTTGTGAGCAAACGTCGTTATCTTACCGGTAaagaa > 1:1891597/1‑38 (MQ=255) ctaTTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGt > 1:2094057/1‑61 (MQ=255) ctaTTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTACggg > 1:3759022/1‑71 (MQ=255) ctaTTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTACggg > 1:1658789/1‑71 (MQ=255) taTTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCa < 1:201424/54‑1 (MQ=255) ttGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCatga > 1:1141853/1‑48 (MQ=255) ttGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCatga > 1:1546399/1‑48 (MQ=255) ttGTGAGGAAACGTCGTTATCTTACCGGTAAAGAAGt < 1:1970489/37‑1 (MQ=255) ttGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGtt < 1:2265399/38‑1 (MQ=255) ttGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGt < 1:3275963/37‑1 (MQ=255) ttGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGt < 1:815460/37‑1 (MQ=255) tgtgAGCAAACGTCGTTATCTTACCGGTAAAGAAGt < 1:2677220/36‑1 (MQ=255) gtgAGTAAACGTCGTTATCTTACCGGTAAAGAAGt > 1:496535/1‑35 (MQ=255) gtgAGTAAACGTCGTTATCTTACCGGTAAAGAAGt > 1:3363858/1‑35 (MQ=255) gtgAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTACGGGGCAAc > 1:2100398/1‑71 (MQ=255) aGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTACGGGGCaa > 1:1653673/1‑67 (MQ=255) tAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTa < 1:1837321/57‑1 (MQ=255) tAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTa < 1:3913093/57‑1 (MQ=255) | TGACCGATTGAGGTTTCCTATAGGTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTACGGGGCAAC > W3110S.gb/4546656‑4546789 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |