| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,549,754 | G→A | 46.5% | intergenic (+43/‑24) | fimC → / → fimD | chaperone, periplasmic/outer membrane usher protein, type 1 fimbrial synthesis |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,549,754 | 0 | G | A | 46.5% | 7.8 / 57.3 | 43 | intergenic (+43/‑24) | fimC/fimD | chaperone, periplasmic/outer membrane usher protein, type 1 fimbrial synthesis |
| Reads supporting (aligned to +/- strand): ref base G (7/16); new base A (11/9); total (18/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.24e-01 | |||||||||||
CAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCT > W3110S.gb/4549687‑4549823 | cAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGt > 1:3168458/1‑71 (MQ=255)cAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGt > 1:164500/1‑71 (MQ=255) aaaaTGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGTCGGGTg < 1:2621393/71‑1 (MQ=255) aTGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCCggagg < 1:2427834/71‑1 (MQ=255) gAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCCGGAGGaa > 1:2858956/1‑70 (MQ=255) gAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGaat < 1:938272/71‑1 (MQ=255) gAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGaat < 1:3197661/71‑1 (MQ=255) aCGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGTCGGGTGATTTTAAGc > 1:3444801/1‑57 (MQ=255) aCGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGTCGGGTGATTTTAAGc > 1:2692213/1‑57 (MQ=255) aCGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGt < 1:1677941/71‑1 (MQ=255) cAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGc > 1:947169/1‑54 (MQ=255) cAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGc > 1:2344935/1‑54 (MQ=255) cAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCCGGAGGAATAATGTCat < 1:345578/71‑1 (MQ=255) cAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCat < 1:2183052/71‑1 (MQ=255) ggggAATTTTTCGCCTGAATAAAAAGAATTGACTGTCGGGTGATTTTAAGCCGGAGGAATAATGTCATATc < 1:1411765/71‑1 (MQ=255) ggggAATTTTTCCCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCCGGAGGaat < 1:3395254/60‑1 (MQ=255) gggAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCt > 1:33858/1‑71 (MQ=255) gggAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTAAAGCCGGAGGAATAATGTCATATCt > 1:312495/1‑71 (MQ=255) ggAATTTTTCGCCTGAATAAAAAGAATTGACTGTCGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTg < 1:3269839/71‑1 (MQ=255) gAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGa < 1:2482821/71‑1 (MQ=255) gAATTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGa < 1:1477343/71‑1 (MQ=255) aaTTTTTCGCCTGAATAAAAAGAATTGACTGTCGGGTGAttt < 1:3426901/42‑1 (MQ=255) aaTTTTTCGCCTGAATAAAAAGAATTGACTGTCGGGTGATTTTAAGCCGGAGGaata < 1:3243236/57‑1 (MQ=255) aaTTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCata > 1:3195902/1‑65 (MQ=255) aaTTTTTCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCata > 1:3924618/1‑65 (MQ=255) aTTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCCGGAGGAATAATGt < 1:3556455/60‑1 (MQ=255) ttttCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCTGGAGGAATAATGTCATATCTGAAt > 1:855033/1‑69 (MQ=255) tttCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCTGGAGGaataa > 1:2938381/1‑54 (MQ=255) tttCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAAttt < 1:1448135/70‑1 (MQ=255) tttCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAAttt > 1:3437946/1‑70 (MQ=255) tttCGCCTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAAttt > 1:1639499/1‑70 (MQ=255) cTGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGa < 1:1863206/60‑1 (MQ=255) tGAATAAAAAGAATTGACTGCCAGGTGATTTTAAGCCggagg > 1:1161664/1‑42 (MQ=255) aaTAAAAAGAATTGACTGCCGGGTGATTTTAAGCTGGAGGAATAATGTCATATCTGAATTTAAGACttt < 1:3463185/69‑1 (MQ=255) aaTAAAAAGAATTGACTGCCGGGTGATTTTAAGCTGGAGGAATAATGTCATATCTGAATTTAAGACttt < 1:3763624/69‑1 (MQ=255) aaTAAAAAGAATTGACTGCCGGGTGATTTTAAGCTGGAGGAATAATGTCATATCTGAATTTAAGACttt < 1:1021252/69‑1 (MQ=255) aaTAAAAAGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGt < 1:374880/48‑1 (MQ=255) tAAAAAGAATTGACTGCCAGGTGATTTTAAGCCgg < 1:816948/35‑1 (MQ=255) aaGAATTGACTGTCGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGa < 1:2944041/71‑1 (MQ=255) aaGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTaa > 1:1285920/1‑57 (MQ=255) aaGAATTGACTGCCAGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTaa > 1:2799701/1‑57 (MQ=255) cTGCCGGGTGATTTTAAGCTGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACaa < 1:3974341/71‑1 (MQ=255) cGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCt < 1:3301734/71‑1 (MQ=255) | CAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCT > W3110S.gb/4549687‑4549823 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |