| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,605,956 | C→T | 33.3% | V82I (GTT→ATT) | dnaT ← | DNA biosynthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,605,956 | 0 | C | T | 33.3% | 41.0 / 29.9 | 36 | V82I (GTT→ATT) | dnaT | DNA biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base C (10/14); new base T (7/5); total (17/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01 | |||||||||||
CAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATCGCTTCCCGGACGCGCCAG > W3110S.gb/4605888‑4606022 | cAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACgg < 1:313464/71‑1 (MQ=255) aGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACggg < 1:2575079/71‑1 (MQ=255) tCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCt > 1:1254369/1‑70 (MQ=255) tCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCt > 1:1313087/1‑70 (MQ=255) gggTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGa > 1:113613/1‑70 (MQ=255) gggTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGa > 1:909557/1‑70 (MQ=255) gggTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGa > 1:1892644/1‑70 (MQ=255) gTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATag < 1:3534128/71‑1 (MQ=255) ccAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATaga < 1:465188/68‑1 (MQ=255) ccAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATaga < 1:758827/68‑1 (MQ=255) tctggATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGa < 1:2509624/57‑1 (MQ=255) tCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCg < 1:1689556/52‑1 (MQ=255) tCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGa < 1:2386709/60‑1 (MQ=255) ccGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCg > 1:989718/1‑71 (MQ=255) cGGATACATGGCGAATTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGt > 1:642801/1‑71 (MQ=255) cGGATACATGGCGAATTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGt > 1:2395640/1‑71 (MQ=255) cGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGt > 1:2926866/1‑71 (MQ=255) cGGATACATGGCGAATTTCCCCATGGGTAAAGCAACGGGAg > 1:3150103/1‑41 (MQ=255) tACATGGCGAATTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTc > 1:917518/1‑68 (MQ=255) aTGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGc < 1:205114/71‑1 (MQ=255) gCGAATTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCg > 1:1794261/1‑40 (MQ=255) gCGAATTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGa > 1:525423/1‑70 (MQ=255) gCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATAGTCCAGAGCGa < 1:3645904/70‑1 (MQ=255) aaTTTCCCCATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATc < 1:1499776/71‑1 (MQ=255) tttCCCCATGGGTACAGCAATGGGAGCGGCTTGCTGTTCCTGATa < 1:3899095/45‑1 (MQ=38) ttCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTCATCGTCCAGAGCGACATCGCt > 1:47225/1‑71 (MQ=255) cccATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATag < 1:3445893/42‑1 (MQ=255) cccATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATag < 1:1721727/42‑1 (MQ=255) cccATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATCGCTTcc < 1:2598812/71‑1 (MQ=255) cccATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATCGCTTcc < 1:1799673/71‑1 (MQ=255) cATGGGTACAGCAATGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATCGCTTCCCg < 1:1646677/71‑1 (MQ=255) tGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATCGCTTCCCGGa > 1:173444/1‑71 (MQ=255) tACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTg < 1:386743/39‑1 (MQ=255) tACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTg < 1:1921937/39‑1 (MQ=255) aCAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGa > 1:2240400/1‑52 (MQ=255) gCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATCGCTTCCCGGACGCGCCAg > 1:1092704/1‑71 (MQ=255) | CAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATCGCTTCCCGGACGCGCCAG > W3110S.gb/4605888‑4606022 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |