| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,616,051 | T→C | 9.7% | intergenic (+366/‑27) | prfC → / → osmY | peptide chain release factor RF‑3/periplasmic protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,616,051 | 0 | T | C | 9.7% | 289.2 / 19.1 | 108 | intergenic (+366/‑27) | prfC/osmY | peptide chain release factor RF‑3/periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base T (55/42); new base C (2/8); total (58/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.28e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
TGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTG > W3110S.gb/4615985‑4616116 | tGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTAc > 1:2549272/1‑69 (MQ=255) cTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCa < 1:1700759/71‑1 (MQ=255) cTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATCACGAGCa < 1:3380493/71‑1 (MQ=255) cTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATCACGAGCaaa < 1:869022/70‑1 (MQ=255) cTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATCACGAGCaaa < 1:4065766/70‑1 (MQ=255) tGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAAt < 1:605096/71‑1 (MQ=255) tGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAAt < 1:2074054/71‑1 (MQ=255) gAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAAt < 1:3214203/70‑1 (MQ=255) aaGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGc > 1:3082977/1‑55 (MQ=255) aaGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAAt > 1:1643913/1‑69 (MQ=255) aaGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAAt > 1:1648427/1‑69 (MQ=255) gTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGATCAAACATAc < 1:677284/61‑1 (MQ=255) tctctcCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCaaa < 1:3130752/55‑1 (MQ=255) tctctcCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCaaa < 1:2062398/55‑1 (MQ=255) tcCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATCGAt > 1:1199283/1‑70 (MQ=255) cccTATTCAGCAATGCAACCTCGTATTGCCAGGCTCAAATAACGAGCAAACATACAGGAATAAATCGATGa > 1:394533/1‑71 (MQ=255) ccTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACAt < 1:1123990/52‑1 (MQ=255) ccTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACAt < 1:1246450/52‑1 (MQ=255) ccTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACAt < 1:1805545/52‑1 (MQ=255) ccTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACAt < 1:2958349/52‑1 (MQ=255) ccTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGAt < 1:3933771/68‑1 (MQ=255) ccTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATCGAt < 1:3064874/68‑1 (MQ=255) ccTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATCGAt < 1:355515/68‑1 (MQ=255) ccTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATCGAt < 1:1482279/68‑1 (MQ=255) ccTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATCGAt < 1:1940260/68‑1 (MQ=255) aTTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGa > 1:3346369/1‑64 (MQ=255) aTTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGa > 1:1474704/1‑64 (MQ=255) aTTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGa > 1:2021416/1‑64 (MQ=255) aTTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGa > 1:2026988/1‑64 (MQ=255) aTTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGa > 1:2969652/1‑64 (MQ=255) ttCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATaaa > 1:1866103/1‑59 (MQ=255) ttCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGa > 1:3767559/1‑63 (MQ=255) gCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTa < 1:1839984/65‑1 (MQ=255) gCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTa < 1:2997753/65‑1 (MQ=255) gCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACaa < 1:1627482/71‑1 (MQ=255) gCAATGCAACCTCGTGTTGCCAGGCTCAAATCACTAGCAAATATACAGGAATAAATCGATGACTATGACaa < 1:23900/71‑1 (MQ=255) aTGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCaaa < 1:3674339/38‑1 (MQ=255) tGCAACCTCGTGTTGCCAGGCTCAAATTACGCGCAAACATAc > 1:761093/1‑42 (MQ=255) tGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACa < 1:1395502/39‑1 (MQ=255) tGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACAt < 1:1491804/40‑1 (MQ=255) tGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACAt < 1:278022/40‑1 (MQ=255) tGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACAt < 1:3443148/40‑1 (MQ=255) tGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGAt < 1:634993/56‑1 (MQ=255) tGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGAt < 1:2000552/56‑1 (MQ=255) tGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGAt < 1:2701442/56‑1 (MQ=255) tGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGa > 1:1946434/1‑64 (MQ=255) tGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGa > 1:2106980/1‑64 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATa > 1:2660061/1‑39 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATa > 1:460987/1‑39 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATaaa > 1:1567994/1‑49 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATaaa > 1:2952378/1‑49 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGAt > 1:2507553/1‑54 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGAt > 1:1849360/1‑54 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGAt > 1:54559/1‑54 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGAt > 1:3532113/1‑54 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGa > 1:2939321/1‑56 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGa > 1:2944007/1‑56 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGa > 1:3097639/1‑56 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTg > 1:3747428/1‑70 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTg > 1:2080188/1‑70 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTg > 1:3460271/1‑70 (MQ=255) ccTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACaa < 1:1966332/62‑1 (MQ=255) tCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATaaa > 1:471206/1‑44 (MQ=255) tCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATa < 1:3729243/42‑1 (MQ=255) tCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATa < 1:1988774/42‑1 (MQ=255) tCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATc < 1:1922543/46‑1 (MQ=255) tCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGa > 1:3169803/1‑51 (MQ=255) tCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGa > 1:1732046/1‑51 (MQ=255) tCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGa > 1:1881201/1‑62 (MQ=255) tCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGa > 1:3343315/1‑62 (MQ=255) tCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGa < 1:3571777/66‑1 (MQ=255) gtgtTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGa > 1:3294814/1‑49 (MQ=255) gtgtTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGa > 1:3952509/1‑49 (MQ=255) tgtTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTAt < 1:17107/52‑1 (MQ=255) tgtTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTAt < 1:3713470/52‑1 (MQ=255) tgtTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTAt < 1:2674979/52‑1 (MQ=255) tgtTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTAt < 1:2726437/52‑1 (MQ=255) ttGCCAGGCTCAAATTACGAGCAAACATACAGGAAt < 1:652422/36‑1 (MQ=255) ttGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGAc < 1:856015/47‑1 (MQ=255) ttGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGAc < 1:311862/47‑1 (MQ=255) ttGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGa > 1:2603135/1‑70 (MQ=255) gCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGa > 1:2372777/1‑41 (MQ=255) gCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTa > 1:3855377/1‑47 (MQ=255) gCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTa > 1:3921236/1‑47 (MQ=255) gCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTa > 1:2548521/1‑47 (MQ=255) aGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGAc < 1:1897936/48‑1 (MQ=255) ggCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAActct > 1:1371429/1‑71 (MQ=255) ggCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAActct > 1:179703/1‑71 (MQ=255) gCTCAAATTACGAGCAAACATACAGGAATAAATCGATGAc < 1:3897066/40‑1 (MQ=255) gCTCAAATTACGAGCAAACATACAGGAATAAATCGATGAc < 1:3460301/40‑1 (MQ=255) tCAAATTACGAGCAAACATACAGGAATAAATCGATg > 1:1526043/1‑36 (MQ=255) tCAAATTACGAGCAAACATACAGGAATAAATCGATGa > 1:2614802/1‑37 (MQ=255) tCAAATTACGAGCAAACATACAGGAATAAATCGATGa > 1:2067586/1‑37 (MQ=255) tCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTctgct > 1:3762837/1‑71 (MQ=255) tCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTctgct > 1:3735534/1‑71 (MQ=255) tCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTctgct > 1:304686/1‑71 (MQ=255) tCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTctgct > 1:3453576/1‑71 (MQ=255) tCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTctgct > 1:1792059/1‑71 (MQ=255) cAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTctgctg > 1:2264095/1‑71 (MQ=255) aaaTTACGAGCAAACATACAGGAATAAATCGATGa > 1:4036630/1‑35 (MQ=255) | TGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTG > W3110S.gb/4615985‑4616116 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |