| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 352,300 | C→T | 56.0% | T124I (ACT→ATT) | prpE → | predicted propionyl‑CoA synthetase with ATPase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 352,300 | 0 | C | T | 56.0% | 12.7 / 65.4 | 50 | T124I (ACT→ATT) | prpE | predicted propionyl‑CoA synthetase with ATPase domain |
| Reads supporting (aligned to +/- strand): ref base C (18/4); new base T (17/11); total (35/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
TCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCCTCGCACAGCG > W3110S.gb/352233‑352368 | tCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTActct > 1:1497344/1‑71 (MQ=255)tCATTGGGTGTGCAGCGCGGCGATCGGGTGATGGTGTATATGCCGATGATTGCCGAAGCGCATATTActct > 1:2533500/1‑71 (MQ=255) gcgcGGCGATCGGGTGCTGGTGTATATGTCGATGATT‑CCGAAGCGCATATTATTCTGCTGGCCTgcgcgcg < 1:1338403/71‑1 (MQ=255) cgcgGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTATTCTGCTGGCCTgcgcgcg < 1:700955/71‑1 (MQ=255) gcggcgATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTATTCTGCTGGCCTgcgcgcg > 1:53844/1‑70 (MQ=255) tCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTATTCTGCTGGCCTGCTCGCGCATTGgcg < 1:2857612/71‑1 (MQ=255) tCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGgcg < 1:450090/71‑1 (MQ=255) tCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGgcg < 1:1929319/71‑1 (MQ=255) tCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTATTCTGCTGGCATGCGCGCGCATTGgcg < 1:3078492/71‑1 (MQ=255) cGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGgcgc > 1:2603523/1‑71 (MQ=255) tGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTAtt < 1:3126381/71‑1 (MQ=255) tGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTAtt < 1:787111/71‑1 (MQ=255) cTGGTGTATATGCCGATGATTGCCGAAGCGCATATTATTCTGCTgg > 1:2741759/1‑46 (MQ=255) ggTGTATATGCCGATGATTGCCGAAGCGCATATTACTctg > 1:77728/1‑40 (MQ=255) ggTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTgcgcgcgc > 1:1152591/1‑55 (MQ=255) ggTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACt > 1:2783054/1‑71 (MQ=255) tataTGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCa < 1:2009968/52‑1 (MQ=255) tatGCCGATGATTGCCTAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCggtgg > 1:165788/1‑71 (MQ=255) tatGCCGATGATTGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCggtgg > 1:3078191/1‑71 (MQ=255) tatGCCGATGATTGCCGAAGCGCATATTACTCTGCTgg > 1:789884/1‑38 (MQ=255) tatGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCAt > 1:3533329/1‑51 (MQ=255) tatGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCggtgg > 1:3025676/1‑71 (MQ=255) tGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGTGCGCGCATTGGCGCTATTCACTCGgtggtg < 1:1775729/71‑1 (MQ=255) tGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCAttt > 1:3427659/1‑50 (MQ=255) tGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGgtggtg > 1:1499472/1‑71 (MQ=255) tGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGgtggtg > 1:2436255/1‑71 (MQ=255) tGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGgtggtg > 1:1595744/1‑71 (MQ=255) gCCGATGATTGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGgtgt > 1:417989/1‑71 (MQ=255) gCCGATGATTGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGgtgt > 1:857098/1‑71 (MQ=255) ccGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGtt > 1:903459/1‑71 (MQ=255) ccGATGATTGCCGAAGCGCATATTACTCTGCCGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGtt > 1:309222/1‑71 (MQ=255) gatTGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTa > 1:1833650/1‑50 (MQ=255) gatTGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTa > 1:34763/1‑50 (MQ=255) ttGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGa < 1:1661925/71‑1 (MQ=255) ttGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGa < 1:1321888/71‑1 (MQ=255) ttGCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGa < 1:1925626/71‑1 (MQ=255) tGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCa > 1:2240960/1‑51 (MQ=255) gCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCa > 1:259484/1‑50 (MQ=255) gCCGAAGCGCATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCa > 1:3658911/1‑50 (MQ=255) gCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTtgg > 1:735636/1‑65 (MQ=255) gCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTtgg > 1:1131432/1‑65 (MQ=255) gcATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCCTCg > 1:1982132/1‑70 (MQ=255) gcATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCCTCg > 1:373659/1‑70 (MQ=255) gcATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCCTCg > 1:3751076/1‑70 (MQ=255) gcATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCCTCg > 1:2234443/1‑70 (MQ=255) gcATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCCTCg > 1:1778622/1‑70 (MQ=255) cATATTATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCggtgg < 1:1113305/49‑1 (MQ=255) attattCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTAGGTGGTGTTTGGTGGATTTGCCTCGCACAg > 1:3294268/1‑71 (MQ=255) ttATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGgtgt > 1:3655808/1‑48 (MQ=255) ttATTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGgtgt > 1:2476058/1‑48 (MQ=255) ttACTCTGCTGGCCTGCGCGCGCATTGGCGCTAtt > 1:2323556/1‑35 (MQ=255) ttACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTCGCCTCGCACAg < 1:1463528/70‑1 (MQ=255) tACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCCTCGCACAGCg > 1:134388/1‑71 (MQ=255) | TCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCCTCGCACAGCG > W3110S.gb/352233‑352368 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |