| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,635,047 | G→A | 31.4% | A53V (GCG→GTG) | yjjK ← | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,635,047 | 0 | G | A | 31.4% | 69.1 / 40.9 | 51 | A53V (GCG→GTG) | yjjK | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (19/16); new base A (10/6); total (29/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCC > W3110S.gb/4634982‑4635111 | gCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCAt < 1:481141/70‑1 (MQ=255)gCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCAt < 1:1849125/70‑1 (MQ=255) aaCCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATgcg > 1:418372/1‑70 (MQ=255) aaCCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATgcg > 1:3217051/1‑70 (MQ=255) aCCAATCTTGATGTCTGGCTGCGGACGCTCTTCACCTTCGATGTCTTTATCAATGCCCACCATAATgcg > 1:2746531/1‑69 (MQ=255) aCCAATCTTGATGTATGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCACCATAATgcg > 1:3320895/1‑69 (MQ=255) cAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAAAGCCCACCATAATGCgcag > 1:2723673/1‑70 (MQ=255) tCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCACCATAATgcgc < 1:165707/65‑1 (MQ=255) ttGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGt > 1:2456202/1‑71 (MQ=255) ttGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGt > 1:2184264/1‑71 (MQ=255) ttGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGt > 1:3245829/1‑71 (MQ=255) gATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTg > 1:1395484/1‑70 (MQ=255) aTGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTgg < 1:1373892/70‑1 (MQ=255) gTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCAt < 1:2155851/51‑1 (MQ=255) tCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATaa > 1:1397429/1‑52 (MQ=255) tCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATaa > 1:2675922/1‑52 (MQ=255) tCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATaa > 1:2612925/1‑52 (MQ=255) tGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATaa > 1:659939/1‑50 (MQ=255) tGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATaa > 1:338909/1‑50 (MQ=255) tGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTAc > 1:192945/1‑71 (MQ=255) ggACGCGCTTCACCTTCGATGTCTTTATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCgcgc < 1:2150416/70‑1 (MQ=255) cTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGa < 1:1053689/71‑1 (MQ=255) cTTCACCTTCGATGTCTTTATCAATGCCCACCATAATGCGCAGCAggg < 1:1925749/48‑1 (MQ=255) aCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGAccc < 1:1957942/70‑1 (MQ=255) aCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGAcc > 1:2434319/1‑69 (MQ=255) aCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGAcc > 1:1153142/1‑69 (MQ=255) ccTTCGATGTCTTTATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAg < 1:3958524/71‑1 (MQ=255) ttCTATGTCTTTATCAATGCCCGCCATAATGCgcagca < 1:1111287/38‑1 (MQ=255) ttCGATGTCTTTATCAATGCCCGCCATAATGCgcagca < 1:1005639/38‑1 (MQ=255) ttCGATGTCTTTATCAATGCCCGCCATAATGCgcagca < 1:3761847/38‑1 (MQ=255) ttCGATGTCTTTATCAATGCCCGCCATAATGCgcagca < 1:938520/38‑1 (MQ=255) ttCGATGTCTTTATCAATGCCCGCCATAATGCgcagca < 1:3377509/38‑1 (MQ=255) ttCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGa > 1:50662/1‑71 (MQ=255) tCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGa > 1:393413/1‑70 (MQ=255) tCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGa > 1:3421855/1‑70 (MQ=255) tCGATGTCTTTATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCGAGGa > 1:3629999/1‑70 (MQ=255) gTCTTTATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAg > 1:4015032/1‑63 (MQ=255) gTCTTTATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAg > 1:2676673/1‑63 (MQ=255) tCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCaa < 1:199268/70‑1 (MQ=255) cTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCaa < 1:1085341/69‑1 (MQ=255) tttATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAAtt > 1:2846479/1‑70 (MQ=255) tttATCAATGCCCACCATAATGCGCAGCAGGGTGGACt < 1:3132099/38‑1 (MQ=255) tttATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTa > 1:4032293/1‑40 (MQ=255) tATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAAtt < 1:985355/68‑1 (MQ=255) tATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCa > 1:2647606/1‑57 (MQ=255) tATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAg > 1:2241044/1‑58 (MQ=255) cAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGcc > 1:393508/1‑71 (MQ=255) aaTGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGccc > 1:3189360/1‑71 (MQ=255) aTGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGcccc < 1:1666482/71‑1 (MQ=255) aTGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGcccc < 1:1454469/71‑1 (MQ=255) aTGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGcccc < 1:810722/71‑1 (MQ=255) | GCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCC > W3110S.gb/4634982‑4635111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |