| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 412,184 | G→A | 14.3% | L932L (CTG→TTG) | sbcC ← | exonuclease, dsDNA, ATP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 412,184 | 0 | G | A | 14.3% | 128.3 / 11.8 | 49 | L932L (CTG→TTG) | sbcC | exonuclease, dsDNA, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (14/28); new base A (3/4); total (17/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
TCGCCGCCGGAAAGGGTACGGGTATCGCGTACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCAGCCGGGTAAGTTGCTGATTAGCGAG > W3110S.gb/412114‑412250 | tCGCCGCCGGAAAGGGTACGGGTATCGCGTACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAg > 1:530423/1‑71 (MQ=255) gccgGAAAGGGTACGGGTATCGCGTACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCt < 1:1945120/71‑1 (MQ=255) ccgGAAAGGGTACGGGTATCGCGTACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCTc < 1:3581738/71‑1 (MQ=255) aCGGGTATCGCGTACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAACGCCTCGCTGGCTTTgc > 1:3635742/1‑71 (MQ=255) tATCGCGTACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGt < 1:2837959/71‑1 (MQ=255) tCGCGTACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTaa > 1:3782251/1‑71 (MQ=255) gcgTACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACa < 1:2903194/71‑1 (MQ=255) gcgTACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACa < 1:2672767/71‑1 (MQ=255) gcgTACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACa < 1:4030558/71‑1 (MQ=255) gcgTACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACa < 1:3877044/71‑1 (MQ=255) gcgTACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACa < 1:2257696/71‑1 (MQ=255) cgTACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAACGCCTCGCTGGCTTTGCTCTGTAACAg < 1:381773/71‑1 (MQ=255) cgTACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAACGCCTCGCTGGCTTTGCGCTGTAACAg < 1:455535/71‑1 (MQ=255) tACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACa < 1:1933599/68‑1 (MQ=255) tACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACa < 1:2493423/68‑1 (MQ=255) aCCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAg > 1:2532529/1‑68 (MQ=255) ccGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGa > 1:2063462/1‑68 (MQ=255) ccGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAg > 1:836090/1‑71 (MQ=255) tCTGCCTGCCATGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACa < 1:3043864/61‑1 (MQ=255) tCTGCCTGCCAGGTATCAACAACCTCGACTTCCAACGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGccc > 1:3828077/1‑71 (MQ=255) tgcctgccAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTg > 1:3986167/1‑43 (MQ=255) tgcctgccAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTg > 1:2867310/1‑43 (MQ=255) tgcctgccAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGAt < 1:2275506/62‑1 (MQ=255) tgcctgccAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGAt < 1:1150329/62‑1 (MQ=255) tgcctgccAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGt > 1:1226934/1‑71 (MQ=255) tgcctgccAGGTATCAACAACCTCGACTTCCAGCGACTCGCTg > 1:1266612/1‑43 (MQ=255) cAGGTATCAACAACCTCGACTTCCAACGCCTCGCTGGCTTTgcgc < 1:3220437/45‑1 (MQ=255) tATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACa < 1:913498/48‑1 (MQ=255) tATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCAGCCGGGTa < 1:955210/71‑1 (MQ=255) tATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCAGCCGGGTa < 1:1091736/71‑1 (MQ=255) tATCAACAACCTCGACTTCCAACGCCTCGCTGGCTTTgcgc < 1:1991284/41‑1 (MQ=255) aTCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAg > 1:1629005/1‑48 (MQ=255) aTCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGa > 1:3714744/1‑49 (MQ=255) tCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACa < 1:349086/46‑1 (MQ=255) tCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACa < 1:453252/46‑1 (MQ=255) tCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGAt > 1:2910583/1‑49 (MQ=255) tCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGAt > 1:2536471/1‑49 (MQ=255) tCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCa < 1:12459/61‑1 (MQ=255) tCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCa < 1:3802972/61‑1 (MQ=255) tCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCAGCCGGGTaa < 1:2007508/70‑1 (MQ=255) aacaacCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTaa > 1:1017947/1‑42 (MQ=255) aacCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCAGCCGGGTAAGTTGCt < 1:3255533/71‑1 (MQ=255) acCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACa < 1:2386029/40‑1 (MQ=255) acCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCa > 1:3077610/1‑55 (MQ=255) cTCGACTTCCAACGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCAGCCGGGTAAGTTGCTGAt > 1:1697417/1‑71 (MQ=255) aCTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCAGCCGGGTAAGTTGCt < 1:2140802/64‑1 (MQ=255) aCTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCAGCAGGGTAAGTTGCt < 1:1219001/64‑1 (MQ=255) ccAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCAGCCGGGTAAGTTGCTGATTAGCgag < 1:3911952/70‑1 (MQ=255) ccAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCAGCCGGGTAAGTTGCTGATTAGCgag < 1:2926550/70‑1 (MQ=255) aGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCAGCCGGGTAAGTTGCt < 1:4030821/58‑1 (MQ=255) | TCGCCGCCGGAAAGGGTACGGGTATCGCGTACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCAGCCGGGTAAGTTGCTGATTAGCGAG > W3110S.gb/412114‑412250 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |