Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 422,530 T→C 17.9% N264N (AAT→AAC malZ → maltodextrin glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb422,5300TC17.9% 87.0 / 13.2 39N264N (AAT→AACmalZmaltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (12/20);  new base C (5/2);  total (17/22)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGAT  >  W3110S.gb/422465‑422596
                                                                 |                                                                   
cGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTgg                                                                >  1:697753/1‑71 (MQ=255)
cGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTgg                                                                >  1:59318/1‑71 (MQ=255)
cGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTgg                                                                <  1:2145955/71‑1 (MQ=255)
      gttcTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACg                                                           <  1:2546390/66‑1 (MQ=255)
                gCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGt                                               <  1:3620735/71‑1 (MQ=255)
                 cGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTg                                                >  1:3669719/1‑69 (MQ=255)
                 cGTGTTAAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTg                                                >  1:519259/1‑69 (MQ=255)
                   tgtTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGggt                                                        <  1:653115/59‑1 (MQ=255)
                   tgtTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGggt                                                        <  1:2159988/59‑1 (MQ=255)
                     tttAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACggg                                                         <  1:3495113/56‑1 (MQ=255)
                     tttAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACggg                                                         <  1:503001/56‑1 (MQ=255)
                     tttAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACggg                                                         <  1:418204/56‑1 (MQ=255)
                     tttAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGggtgg                                                      >  1:2293085/1‑59 (MQ=255)
                     tttAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGggt                                                        <  1:1719605/57‑1 (MQ=255)
                     tttAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACaa                                          >  1:2231014/1‑71 (MQ=255)
                     tttAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACaa                                          >  1:1331361/1‑71 (MQ=255)
                       tAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAACCGTGGCACGGGTGGTGCTTGTCACAAcc                                        >  1:3752683/1‑71 (MQ=255)
                               gTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCg                                     <  1:3597092/66‑1 (MQ=255)
                                     aTTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCTAATCGCCCTgg                          <  1:3173997/71‑1 (MQ=255)
                                       tCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGgcg                        >  1:3564429/1‑71 (MQ=255)
                                        cccATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAAcc                                        >  1:4069727/1‑54 (MQ=255)
                                        cccATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGgcgc                       >  1:84862/1‑71 (MQ=255)
                                        cccATGCCTGGTTTGACAGGCATAACCGTGGCTCGGGTGGTGCTTGTCACaa                                          >  1:183290/1‑52 (MQ=255)
                                        cccATGCCTGGTTTGACAGGCATAACCGTGGCACgg                                                          >  1:3939523/1‑36 (MQ=255)
                                        cccATGCCTGGTTTGACAGGCATAACCGTGGCACGGGTGGTGCTTGTCACaa                                          >  1:3521636/1‑52 (MQ=255)
                                        cccATGCCTGGTTTGACAGGCATAACCGTGGCACGGGTGGTGCTTGTCACaa                                          >  1:290570/1‑52 (MQ=255)
                                         ccATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGgcgc                       <  1:1638292/70‑1 (MQ=255)
                                              ccTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTgg                 <  1:508209/71‑1 (MQ=255)
                                                     tGACAGGCATAACCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCg           <  1:2454599/70‑1 (MQ=255)
                                                     tGACAGGCATAACCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCg           <  1:2054528/70‑1 (MQ=255)
                                                       aCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGtt         <  1:2751722/70‑1 (MQ=255)
                                                             aTAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGtgat  <  1:3445891/71‑1 (MQ=255)
                                                             aTAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGtgat  <  1:3351102/71‑1 (MQ=255)
                                                             aTAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGtgat  <  1:2228469/71‑1 (MQ=255)
                                                             aTAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGtgat  <  1:1968490/71‑1 (MQ=255)
                                                             aTAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGtgat  <  1:1589966/71‑1 (MQ=255)
                                                                aTCGTGGCACGGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGtgat  <  1:3718688/69‑1 (MQ=255)
                                                                aTCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTa               >  1:3671799/1‑55 (MQ=255)
                                                                aTCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTa               >  1:1565654/1‑55 (MQ=255)
                                                                 tCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGtgat  <  1:1659713/67‑1 (MQ=255)
                                                                 |                                                                   
CGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGAT  >  W3110S.gb/422465‑422596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: