Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	422,530	T→C	17.9%	N264N (AAT→AAC)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	422,530	0	T	C	17.9%	87.0 / 13.2	39	N264N (AAT→AAC)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (12/20);  new base C (5/2);  total (17/22)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCAC‑GGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGAT  >  W3110S.gb/422465‑422596                                                                  |                                                                    cGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTgg                                                                >  1:697753/1‑71 (MQ=255) cGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTgg                                                                >  1:59318/1‑71 (MQ=255) cGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTgg                                                                <  1:2145955/71‑1 (MQ=255)       gttcTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCAC‑g                                                           <  1:2546390/66‑1 (MQ=255)                 gCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCAC‑GGGTGGTGCTTGt                                               <  1:3620735/71‑1 (MQ=255)                  cGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCAC‑GGGTGGTGCTTg                                                >  1:3669719/1‑69 (MQ=255)                  cGTGTTAAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCAC‑GGGTGGTGCTTg                                                >  1:519259/1‑69 (MQ=255)                    tgtTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCAC‑Gggt                                                        <  1:653115/59‑1 (MQ=255)                    tgtTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCAC‑Gggt                                                        <  1:2159988/59‑1 (MQ=255)                      tttAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCAC‑ggg                                                         <  1:3495113/56‑1 (MQ=255)                      tttAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCAC‑ggg                                                         <  1:503001/56‑1 (MQ=255)                      tttAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCAC‑ggg                                                         <  1:418204/56‑1 (MQ=255)                      tttAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCAC‑Gggtgg                                                      >  1:2293085/1‑59 (MQ=255)                      tttAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCAC‑Gggt                                                        <  1:1719605/57‑1 (MQ=255)                      tttAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCAC‑GGGTGGTGCTTGTCACaa                                          >  1:2231014/1‑71 (MQ=255)                      tttAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCAC‑GGGTGGTGCTTGTCACaa                                          >  1:1331361/1‑71 (MQ=255)                        tAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAACCGTGGCAC‑GGGTGGTGCTTGTCACAAcc                                        >  1:3752683/1‑71 (MQ=255)                                gTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCAC‑GGGTGGTGCTTGTCACAACCCCg                                     <  1:3597092/66‑1 (MQ=255)                                      aTTCCCATGCCTGGTTTGACAGGCATAATCGTGGCAC‑GGGTGGTGCTTGTCACAACCCCTAATCGCCCTgg                          <  1:3173997/71‑1 (MQ=255)                                        tCCCATGCCTGGTTTGACAGGCATAATCGTGGCAC‑GGGTGGTGCTTGTCACAACCCCGAATCGCCCTGgcg                        >  1:3564429/1‑71 (MQ=255)                                         cccATGCCTGGTTTGACAGGCATAATCGTGGCAC‑GGGTGGTGCTTGTCACAAcc                                        >  1:4069727/1‑54 (MQ=255)                                         cccATGCCTGGTTTGACAGGCATAATCGTGGCAC‑GGGTGGTGCTTGTCACAACCCCGAATCGCCCTGgcgc                       >  1:84862/1‑71 (MQ=255)                                         cccATGCCTGGTTTGACAGGCATAACCGTGGCTC‑GGGTGGTGCTTGTCACaa                                          >  1:183290/1‑52 (MQ=255)                                         cccATGCCTGGTTTGACAGGCATAACCGTGGCAC‑gg                                                          >  1:3939523/1‑36 (MQ=255)                                         cccATGCCTGGTTTGACAGGCATAACCGTGGCAC‑GGGTGGTGCTTGTCACaa                                          >  1:3521636/1‑52 (MQ=255)                                         cccATGCCTGGTTTGACAGGCATAACCGTGGCAC‑GGGTGGTGCTTGTCACaa                                          >  1:290570/1‑52 (MQ=255)                                          ccATGCCTGGTTTGACAGGCATAATCGTGGCAC‑GGGTGGTGCTTGTCACAACCCCGAATCGCCCTGgcgc                       <  1:1638292/70‑1 (MQ=255)                                               ccTGGTTTGACAGGCATAATCGTGGCAC‑GGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTgg                 <  1:508209/71‑1 (MQ=255)                                                      tGACAGGCATAACCGTGGCAC‑GGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCg           <  1:2454599/70‑1 (MQ=255)                                                      tGACAGGCATAACCGTGGCAC‑GGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCg           <  1:2054528/70‑1 (MQ=255)                                                        aCAGGCATAATCGTGGCAC‑GGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGtt         <  1:2751722/70‑1 (MQ=255)                                                              aTAATCGTGGCAC‑GGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGtgat  <  1:3445891/71‑1 (MQ=255)                                                              aTAATCGTGGCAC‑GGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGtgat  <  1:3351102/71‑1 (MQ=255)                                                              aTAATCGTGGCAC‑GGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGtgat  <  1:2228469/71‑1 (MQ=255)                                                              aTAATCGTGGCAC‑GGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGtgat  <  1:1968490/71‑1 (MQ=255)                                                              aTAATCGTGGCAC‑GGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGtgat  <  1:1589966/71‑1 (MQ=255)                                                                 aTCGTGGCACGGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGtgat  <  1:3718688/69‑1 (MQ=255)                                                                 aTCGTGGCAC‑GGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTa               >  1:3671799/1‑55 (MQ=255)                                                                 aTCGTGGCAC‑GGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTa               >  1:1565654/1‑55 (MQ=255)                                                                  tCGTGGCAC‑GGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGtgat  <  1:1659713/67‑1 (MQ=255)                                                                  |                                                                    CGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCAC‑GGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGAT  >  W3110S.gb/422465‑422596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑