| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 430,031 | G→A | 14.3% | C68Y (TGC→TAC) | yajD → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 430,031 | 0 | G | A | 14.3% | 186.9 / 20.2 | 70 | C68Y (TGC→TAC) | yajD | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (31/29); new base A (6/4); total (37/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
CACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGAT > W3110S.gb/429964‑430101 | cacaTTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCa > 1:1476713/1‑71 (MQ=255) ttGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGAt > 1:2434965/1‑71 (MQ=255) ttGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGAt > 1:2657150/1‑71 (MQ=255) ttGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGAt > 1:1494803/1‑71 (MQ=255) tGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCa > 1:790638/1‑66 (MQ=255) gATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCa > 1:281048/1‑71 (MQ=255) gATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTACCACGATCa > 1:1150748/1‑71 (MQ=255) gATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTACCACGATCa > 1:432024/1‑71 (MQ=255) gATCAAGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATc < 1:1560581/70‑1 (MQ=255) aTCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCAt < 1:2402669/71‑1 (MQ=255) aTCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCAt < 1:2326271/71‑1 (MQ=255) aTCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCAt < 1:3821961/71‑1 (MQ=255) tCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATg < 1:3439888/71‑1 (MQ=255) tCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATg < 1:1295963/71‑1 (MQ=255) aCGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAg > 1:1756243/1‑71 (MQ=255) aTACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCg < 1:1408589/71‑1 (MQ=255) tACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGa < 1:2679041/71‑1 (MQ=255) tACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGa < 1:823666/71‑1 (MQ=255) tACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGa < 1:1131568/71‑1 (MQ=255) aCCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGa > 1:1891425/1‑70 (MQ=255) ccAATAACCCGGAAGATGGCAGTAGCTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGaaa > 1:38122/1‑71 (MQ=255) ccAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCa > 1:3745941/1‑58 (MQ=255) ccAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCa > 1:942269/1‑58 (MQ=255) ccAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGaaa > 1:3035570/1‑71 (MQ=255) ccAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGaaa > 1:3798515/1‑71 (MQ=255) ccAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGaaa > 1:3524221/1‑71 (MQ=255) ccAATAACCCGGAAGATGGCAGTAACTGGGAATTGTT‑TGTCTCTATTGCCACGATCa > 1:4063583/1‑57 (MQ=255) cAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGaaa < 1:362838/70‑1 (MQ=255) aaTAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAat > 1:734290/1‑70 (MQ=255) tAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAAtata > 1:2866988/1‑71 (MQ=255) tAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTACCACGATCATGAGCATTCGAAAtata > 1:2860479/1‑71 (MQ=255) tAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTACCACGATCATGAGCATTCGAAAtata > 1:2519533/1‑71 (MQ=255) aaCCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATAc > 1:1286856/1‑71 (MQ=255) aCCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATAcc > 1:3327924/1‑71 (MQ=255) aCCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATAcc > 1:1804118/1‑71 (MQ=255) aCCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTACCACGATCATGAGCATTCGAAATATAcc < 1:3670252/71‑1 (MQ=255) gATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGa > 1:427783/1‑71 (MQ=255) tGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGAt > 1:2953966/1‑70 (MQ=255) gCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAg < 1:1982798/71‑1 (MQ=255) cAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGt < 1:244936/71‑1 (MQ=255) aGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTa > 1:2056673/1‑71 (MQ=255) tAACTGGGAATTGTTGTGTCTCTATTACCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAg < 1:2781636/67‑1 (MQ=255) cTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAatat < 1:3991740/49‑1 (MQ=255) cTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAatat < 1:446286/49‑1 (MQ=255) cTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAata < 1:1187826/48‑1 (MQ=255) tGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGaaa < 1:2287924/45‑1 (MQ=255) gggAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAAtata > 1:2763832/1‑48 (MQ=255) gggAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAAtata > 1:2577712/1‑48 (MQ=255) gggAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGt > 1:3645140/1‑63 (MQ=255) gggAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTAc > 1:1716646/1‑70 (MQ=255) gggAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTAc > 1:2667459/1‑70 (MQ=255) ggAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATAc > 1:2948467/1‑48 (MQ=255) gAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAatat < 1:574045/45‑1 (MQ=255) ttgttgTGTCTCTATTGCCACGATCATGAGCATTCGaaa < 1:274940/39‑1 (MQ=255) ttgttgTGTCTCTATTGCCACGATCATGAGCATTCGaaa < 1:3420166/39‑1 (MQ=255) ttgttgTGTCTCTATTGCCACGATCATGAGCATTCGaaa < 1:2474022/39‑1 (MQ=255) ttgttgTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGt < 1:2506485/71‑1 (MQ=255) gttgtGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGTGGATCAGTATGGTACGACCGTTa > 1:2373184/1‑71 (MQ=255) gttgtGTCTCTATTACCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTa > 1:488053/1‑71 (MQ=255) gttgtGTCTCTATTACCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTa > 1:255347/1‑71 (MQ=255) tgtCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCg < 1:117140/70‑1 (MQ=255) tctATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATgg > 1:928580/1‑52 (MQ=255) tATTACCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAg < 1:1641024/45‑1 (MQ=255) tATTACCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAg < 1:771165/45‑1 (MQ=255) ttGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGt < 1:2352918/57‑1 (MQ=255) ttGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAgggg < 1:1890639/68‑1 (MQ=255) ttGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAgggg < 1:3162990/68‑1 (MQ=255) ttGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGaa < 1:3861755/70‑1 (MQ=255) ttGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGaa < 1:452813/70‑1 (MQ=255) ttGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGaa < 1:3402899/70‑1 (MQ=255) gCCACGATCGTGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGa > 1:267906/1‑70 (MQ=255) gCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTa > 1:3896453/1‑57 (MQ=255) gCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTa > 1:3480619/1‑57 (MQ=255) gCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTa > 1:2713012/1‑57 (MQ=255) gCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGa > 1:495491/1‑70 (MQ=255) gCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGAt > 1:28632/1‑71 (MQ=255) gCCAAGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGAt > 1:2612141/1‑71 (MQ=255) | CACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTATGGTACGACCGTTATCGCAGGGGAAGAT > W3110S.gb/429964‑430101 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |