| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 516,807 | C→T | 36.6% | E233K (GAA→AAA) | ybbN ← | predicted thioredoxin domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 516,807 | 0 | C | T | 36.6% | 37.2 / 39.4 | 41 | E233K (GAA→AAA) | ybbN | predicted thioredoxin domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (18/8); new base T (11/4); total (29/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
CGCGTCTGACCGTCTGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCATCTTCTGGA > W3110S.gb/516740‑516868 | cgcgTCTGACCGTCTGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCAt < 1:160597/70‑1 (MQ=255) cgTCTGACCGTCTGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTTATTgc < 1:1314109/71‑1 (MQ=255) tGACCGTCTGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGcc > 1:2484972/1‑70 (MQ=255) tGACCGTCTGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGcc > 1:744631/1‑70 (MQ=255) tGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGAt > 1:672406/1‑71 (MQ=255) tGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGAt > 1:3610763/1‑71 (MQ=255) gcggTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAg > 1:2955242/1‑71 (MQ=255) gTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTg > 1:3470256/1‑71 (MQ=255) gTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTg > 1:1884469/1‑71 (MQ=255) gagaTCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGa > 1:317691/1‑61 (MQ=255) gagaTCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGa > 1:312928/1‑61 (MQ=255) gaTCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAg > 1:3901314/1‑70 (MQ=255) tACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTTATTGCGCCCAACCTGATGCa > 1:3827722/1‑57 (MQ=255) tACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGcc < 1:3146607/68‑1 (MQ=255) tACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATACAGTTGCAGCGcc < 1:2056667/68‑1 (MQ=255) aCGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGtt > 1:3136956/1‑71 (MQ=255) aCGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGtt > 1:2877455/1‑71 (MQ=255) cAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCg < 1:254622/71‑1 (MQ=255) cAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGAAGCGCCAGTTGCg < 1:1186458/71‑1 (MQ=255) aTGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGt < 1:1742545/52‑1 (MQ=255) aTGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGt < 1:3411565/52‑1 (MQ=255) tGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGa > 1:1291824/1‑45 (MQ=255) tGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCa > 1:1331413/1‑55 (MQ=255) gCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCa > 1:1687816/1‑54 (MQ=255) gCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGc > 1:30336/1‑70 (MQ=255) cccGAACAGCAACTCCAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGcc < 1:1391112/70‑1 (MQ=255) cGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGt > 1:139949/1‑71 (MQ=255) cAGCAACTCCAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCgg > 1:2068884/1‑71 (MQ=255) cAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCgg > 1:382617/1‑71 (MQ=255) cAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCg > 1:200957/1‑70 (MQ=255) aGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGcc < 1:184282/51‑1 (MQ=255) aaCTCCAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCAtc > 1:403502/1‑71 (MQ=255) cTCCAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCAtc < 1:2153441/69‑1 (MQ=255) tCCAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCAtctt > 1:3321555/1‑70 (MQ=255) tCCAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCAtctt > 1:3506513/1‑70 (MQ=255) ccAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTg > 1:1604249/1‑37 (MQ=255) ccAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTg > 1:1941603/1‑37 (MQ=255) gcCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCATCTTCTGGa > 1:3728830/1‑69 (MQ=255) tctTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCATCTTCTg < 1:1228857/64‑1 (MQ=255) tCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAg > 1:480422/1‑47 (MQ=255) tCATTGCGCCCAACCTGATGAAGTTGCAGCGCCAGTTGCGTCGCCAg > 1:3377125/1‑47 (MQ=255) | CGCGTCTGACCGTCTGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCATCTTCTGGA > W3110S.gb/516740‑516868 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |