| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 58,390 | C→T | 11.4% | intergenic (+211/‑84) | djlA → / → yabP | DnaJ‑like protein, membrane anchored/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 58,390 | 0 | C | T | 11.4% | 98.4 / 4.2 | 35 | intergenic (+211/‑84) | djlA/yabP | DnaJ‑like protein, membrane anchored/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (13/18); new base T (2/2); total (15/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGCATCATGAGTTTTTAACTCAA > W3110S.gb/58321‑58458 | ttGACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAaca > 1:2903295/1‑71 (MQ=255)ttGACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAaca > 1:259966/1‑71 (MQ=255) aCCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAATACAt < 1:3386166/71‑1 (MQ=255) ccTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAaca < 1:3468377/66‑1 (MQ=255) ccTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAaca < 1:2326389/66‑1 (MQ=255) tGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACAt < 1:705375/67‑1 (MQ=255) gTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAaca < 1:2082183/56‑1 (MQ=255) gagaGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAATACATACCTGAATGAGCGAt > 1:1536709/1‑71 (MQ=255) agCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGAtttt > 1:2320952/1‑71 (MQ=255) cTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGAttt < 1:726225/68‑1 (MQ=255) cTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGAttt < 1:573457/68‑1 (MQ=255) gacgaGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGta < 1:1648470/71‑1 (MQ=255) acgaGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGta > 1:1016839/1‑70 (MQ=255) gAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTAtatttatt < 1:69747/71‑1 (MQ=255) gAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTAtatttatt < 1:3142955/71‑1 (MQ=255) aTTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTAtatttatt > 1:3818169/1‑69 (MQ=255) ttATATTTTTACGTCTTAAAAATAAAAAATACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAg < 1:3091554/71‑1 (MQ=255) ttATATTTTTACGTCTTAAAAATAAAAAACACATACCTg < 1:1831714/39‑1 (MQ=255) ttATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAg < 1:494075/71‑1 (MQ=255) atatTTTTACGTCTTAAAAATAAAAAATACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGaa > 1:2688768/1‑71 (MQ=255) atatTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGaa > 1:3358535/1‑71 (MQ=255) tttttACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAg < 1:1245617/66‑1 (MQ=255) tttACGTCTTAAAAATAAAAACCACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAAcgcg > 1:2907644/1‑70 (MQ=255) tttACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGaa > 1:4041643/1‑66 (MQ=255) tttACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGaa > 1:222895/1‑66 (MQ=255) tACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGaa > 1:2476267/1‑64 (MQ=255) cTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGta < 1:842450/46‑1 (MQ=255) cTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGta < 1:1087614/46‑1 (MQ=255) ttAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTAtattt < 1:1829723/50‑1 (MQ=255) aaaaataaaaaACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGcatcat > 1:2254794/1‑66 (MQ=255) aaataaaaaACACATACCTGAATGAGCGATTTTTGAAAg < 1:2334554/39‑1 (MQ=255) aaataaaaaACACATACCTGAATGAGCGATTTTTGAAAg < 1:1317820/39‑1 (MQ=255) taaaaaACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGCATCATGAGTTTTTaa < 1:1796901/71‑1 (MQ=255) aaaaaCACATAGCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGCATCATGAGTTTTTAACt > 1:1359165/1‑71 (MQ=255) aaaaaCACATACCTGAATGAGCGATTTTTGAAAGTAtattta > 1:485853/1‑42 (MQ=255) aaaaaCACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGCATCATGAGTTTTTAACt > 1:3805310/1‑71 (MQ=255) aaaaaCACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGCATCATGAGTTTTTAACt > 1:130837/1‑71 (MQ=255) aaaaCACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGCATCATGAGTTTTTAACTc < 1:3673496/71‑1 (MQ=255) aaaaCACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGAGCATCATGAGTTTTTAACTc < 1:3014429/71‑1 (MQ=255) aaCACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGCATCATGAGTTTTTAACTCaa < 1:817993/71‑1 (MQ=255) | TTGACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGCATCATGAGTTTTTAACTCAA > W3110S.gb/58321‑58458 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |