| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 517,941 | A→G | 23.1% | S145P (TCA→CCA) | ybbO ← | predicted oxidoreductase with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 517,941 | 0 | A | G | 23.1% | 45.4 / 11.7 | 26 | S145P (TCA→CCA) | ybbO | predicted oxidoreductase with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base A (12/8); new base G (4/2); total (16/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
GCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGT > W3110S.gb/517871‑518006 | gCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCgatga < 1:3426357/71‑1 (MQ=255) gcATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGGTGtcatca > 1:2575702/1‑70 (MQ=255) gcATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATcac < 1:2356448/71‑1 (MQ=255) cATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGtc < 1:2976653/65‑1 (MQ=255) tatTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGt < 1:2937683/62‑1 (MQ=255) gCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAAt > 1:248719/1‑62 (MQ=255) gCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAAt > 1:3663530/1‑62 (MQ=255) cgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGt < 1:417806/49‑1 (MQ=255) aGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGatgatg > 1:243907/1‑44 (MQ=255) aGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGGTGTCATCACAATACGCCCTTCACCGTGCg > 1:1018358/1‑71 (MQ=255) aGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATa > 1:122681/1‑55 (MQ=255) cgcCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATcaca < 1:335514/50‑1 (MQ=255) gcCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGt > 1:3413030/1‑70 (MQ=255) aCGACCCGGCGTGGAGATTAATCCCATCACCgatgat > 1:1750440/1‑37 (MQ=255) aCGACCCGGCGTGGAGATTAATCCCATCACCgatgat > 1:949544/1‑37 (MQ=255) aCGACCCGGCGTGGAGATTAATCCCATCACCgatgat > 1:2946804/1‑37 (MQ=255) gCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGt < 1:1642686/71‑1 (MQ=255) gagaTTAATCCCATCACCGATGGTGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAg > 1:2896437/1‑71 (MQ=255) tAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCa > 1:2356688/1‑71 (MQ=255) aaTCCCATCACCGATGGTGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCa < 1:1668565/70‑1 (MQ=255) cACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCggg > 1:2837362/1‑51 (MQ=255) cACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCggg > 1:1314809/1‑51 (MQ=255) aCCGATGGTGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTg > 1:710081/1‑71 (MQ=255) aCCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTg > 1:2184134/1‑71 (MQ=255) aCCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTg > 1:2069286/1‑71 (MQ=255) aCCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTg > 1:3615067/1‑71 (MQ=255) aCCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTg > 1:1781127/1‑71 (MQ=255) aCCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTg > 1:977756/1‑71 (MQ=255) ccGATGGTGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTgg < 1:1965999/71‑1 (MQ=255) cGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGgt < 1:3851160/71‑1 (MQ=255) cGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGgt < 1:612293/71‑1 (MQ=255) | GCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGT > W3110S.gb/517871‑518006 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |