| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 546,484 | G→T | 27.4% | G194V (GGC→GTC) | fdrA → | predicted acyl‑CoA synthetase with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 546,484 | 0 | G | T | 27.4% | 92.4 / 17.5 | 40 | G194V (GGC→GTC) | fdrA | predicted acyl‑CoA synthetase with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (14/15); new base T (5/6); total (19/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GGGCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTGCGCTGGC > W3110S.gb/546417‑546550 | gggCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCaa < 1:222060/71‑1 (MQ=255)gggCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCaa < 1:3276909/71‑1 (MQ=255)gggCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCaa < 1:254273/71‑1 (MQ=255)gggCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCaa < 1:3431685/71‑1 (MQ=255) gCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGt > 1:159013/1‑70 (MQ=255) cGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGt < 1:15210/69‑1 (MQ=255) cGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGt < 1:1906938/69‑1 (MQ=255) cGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGt < 1:2149163/69‑1 (MQ=255) cGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCa > 1:2020928/1‑71 (MQ=255) cGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCa > 1:2147017/1‑71 (MQ=255) aCGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAatat > 1:3385644/1‑59 (MQ=255) aCGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTgg > 1:173310/1‑71 (MQ=255) cGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTgg > 1:3457028/1‑70 (MQ=255) tCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGgcgc > 1:875387/1‑71 (MQ=255) tCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGgcgc > 1:4047683/1‑71 (MQ=255) tCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGgcgc > 1:3190309/1‑71 (MQ=255) tGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTa > 1:725687/1‑71 (MQ=255) cacCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCt < 1:2227707/56‑1 (MQ=255) cacCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCt < 1:2173432/56‑1 (MQ=255) cacCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCt < 1:553698/56‑1 (MQ=255) cacCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCt < 1:2792525/56‑1 (MQ=255) cacCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCaa < 1:3320809/37‑1 (MQ=255) cacCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCaa < 1:3746026/37‑1 (MQ=255) acCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCAt > 1:1071904/1‑48 (MQ=255) acCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCAt > 1:1751721/1‑48 (MQ=255) acCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCAATGGCGCTTCCGGTACCGGGATTc < 1:3047062/71‑1 (MQ=255) ccGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCtt > 1:2699728/1‑55 (MQ=255) ccGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTc < 1:3415748/45‑1 (MQ=255) gCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAgg < 1:655255/71‑1 (MQ=255) cTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAg > 1:3785740/1‑69 (MQ=255) cTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAg > 1:124941/1‑69 (MQ=255) cTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCAGGTACCGGGATTCAg > 1:2756947/1‑69 (MQ=255) ttGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTgtgt > 1:4719/1‑71 (MQ=255) ttGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTgtgt > 1:2459002/1‑71 (MQ=255) cTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCg < 1:2864417/71‑1 (MQ=255) cTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCg < 1:3801206/71‑1 (MQ=255) cTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCg < 1:2345192/71‑1 (MQ=255) aCGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCa < 1:2723603/70‑1 (MQ=255) aCGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCa < 1:2268944/70‑1 (MQ=255) ccGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGa > 1:3710716/1‑64 (MQ=255) ggCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTGCGCTGGc < 1:1469151/68‑1 (MQ=255) | GGGCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTGCGCTGGC > W3110S.gb/546417‑546550 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |