| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 594,454 | T→C | 33.3% | K71K (AAA→AAG) | cusR ← | DNA‑binding response regulator in two‑component regulatory system with CusS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 594,454 | 0 | T | C | 33.3% | 40.0 / 34.6 | 39 | K71K (AAA→AAG) | cusR | DNA‑binding response regulator in two‑component regulatory system with CusS |
| Reads supporting (aligned to +/- strand): ref base T (10/16); new base C (8/5); total (18/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
AACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATT > W3110S.gb/594389‑594523 | aaCTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCCTTAt > 1:441725/1‑70 (MQ=255) cTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTgg < 1:911489/71‑1 (MQ=255) cTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTa < 1:3426721/71‑1 (MQ=255) gACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCCTTATTGGCGGAGCGTaa < 1:3594221/69‑1 (MQ=255) aTGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCttt < 1:3696681/48‑1 (MQ=255) gTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCtt < 1:2809695/45‑1 (MQ=255) gTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCtt < 1:380839/45‑1 (MQ=255) gTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCtt < 1:2050579/45‑1 (MQ=255) ttCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTaa < 1:2207682/60‑1 (MQ=255) ttCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACg < 1:3893812/70‑1 (MQ=255) ttCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACg < 1:1708120/70‑1 (MQ=255) ttCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGa < 1:2174567/71‑1 (MQ=255) ttCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGa < 1:2256765/71‑1 (MQ=255) ttCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGa < 1:2585037/71‑1 (MQ=255) ttCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGa < 1:3095457/71‑1 (MQ=255) cAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGata > 1:154722/1‑71 (MQ=255) cAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGa > 1:3613867/1‑69 (MQ=255) cAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGAACGata > 1:1203211/1‑71 (MQ=255) tGCCAAGCGCGGAAAGCAACAGAATCGGCATCCCttt < 1:1292655/37‑1 (MQ=255) ccAAGCGCGGTAAGCAACAGAATCGGCATCCCCTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGcc > 1:3224372/1‑71 (MQ=255) cgcgGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCa > 1:1755418/1‑71 (MQ=255) gTAAGCAACAGAATCGGCATCCCCTTATTGGCGGAGCGTAACATGCGCACGa < 1:1857903/52‑1 (MQ=255) tAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTcc < 1:3666408/71‑1 (MQ=255) tAAGCAACAGAATCGGCATCCCCTTATTGGCGGAGCGTa < 1:1603709/39‑1 (MQ=255) cAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATg > 1:4070906/1‑40 (MQ=255) cAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCgg > 1:111665/1‑69 (MQ=255) cAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCgg > 1:2637146/1‑69 (MQ=255) cAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCgg > 1:3302053/1‑69 (MQ=255) aCAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGgcagc < 1:27531/71‑1 (MQ=255) aCAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGgcagc < 1:2547865/71‑1 (MQ=255) aCAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGgcagc < 1:1478955/71‑1 (MQ=255) aCAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCAGgcagc < 1:519818/71‑1 (MQ=255) cAGAATCGGCATCCCCTTATTGGCGGAGCGTAACATGCGCACGata > 1:1734013/1‑46 (MQ=255) cAGAATCGGCATCCCCTTATTGGCGGAGCGTAACATGCGCACGata > 1:141797/1‑46 (MQ=255) cAGAATCGGCATCCCCTTATTGGCGGAGCGTAACATGCGCACGata > 1:1685064/1‑46 (MQ=255) cAGAATCGGCATCCCCTTATTGGCGGAGCGTAACATGCGCACGata > 1:3490509/1‑46 (MQ=255) cAGAATCGGCATCACCTTATTGGCGGAGCGTAACATGCGCACGata > 1:3181142/1‑46 (MQ=255) aGAATCGGCATCCCCTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCat < 1:2362522/71‑1 (MQ=255) aGAATCGGCATCCCCTTATTGGCGGAGCATAACATGCGCACGata > 1:2749779/1‑45 (MQ=255) aTCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGgcagca < 1:781507/67‑1 (MQ=255) ggCATCCCCTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATc < 1:647322/71‑1 (MQ=255) ccTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGAt > 1:3460641/1‑71 (MQ=255) ccTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGAt > 1:3224244/1‑71 (MQ=255) cTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGAtt < 1:328960/71‑1 (MQ=255) | AACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATT > W3110S.gb/594389‑594523 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |