| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 633,015 | G→T | 51.5% | V69V (GTG→GTT) | ybdL → | methionine aminotransferase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 633,015 | 0 | G | T | 51.5% | 13.5 / 58.6 | 33 | V69V (GTG→GTT) | ybdL | methionine aminotransferase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (6/10); new base T (12/5); total (18/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.44e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.40e-01 | |||||||||||
TACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGC > W3110S.gb/632951‑633084 | tACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTTCAGGcc > 1:2037178/1‑71 (MQ=255)tACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTTCAGGcc > 1:2042187/1‑71 (MQ=255)tACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGcc > 1:2160033/1‑71 (MQ=255) ccacGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGc < 1:3214901/51‑1 (MQ=255) ccacGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGc < 1:957233/51‑1 (MQ=255) ccacGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGc < 1:3542252/51‑1 (MQ=255) aGGGGGCAAACCAATACGCGCCCCTGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGaa > 1:2300613/1‑71 (MQ=255) aGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGaa > 1:4060674/1‑71 (MQ=255) aGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGaa > 1:225510/1‑71 (MQ=255) aGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGaa > 1:3651619/1‑71 (MQ=255) gggggCAAACCAATACGCGCCCATGACCGGCGTTCAGGCCTTGCGCGAGGCGa > 1:3476830/1‑53 (MQ=255) gggggCAAACCAATACGCGCACATGACCGGCGTTCAGGCCTTGCGCGAGGCGa > 1:2815670/1‑53 (MQ=255) cAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTg < 1:1464019/71‑1 (MQ=255) aaaCCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTg < 1:981428/70‑1 (MQ=255) aaaCCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTg < 1:2430510/70‑1 (MQ=255) ccAATACGCGCCCATGACCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATg > 1:2694269/1‑71 (MQ=255) ccAATACGCGCCCATGACCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATg > 1:3837509/1‑71 (MQ=255) ccAATACGCGCCCATGACCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATg > 1:898035/1‑71 (MQ=255) ccAATACGCGCCCATGACCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATg > 1:2962754/1‑71 (MQ=255) tACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTg < 1:2726242/63‑1 (MQ=255) cgcgCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTAt > 1:1292163/1‑70 (MQ=255) ccATGACCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCa < 1:1200115/71‑1 (MQ=255) ccATGACCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCa < 1:2511970/71‑1 (MQ=255) ccATGACCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCa < 1:2359922/71‑1 (MQ=255) tGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCa < 1:4037060/68‑1 (MQ=255) aCCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTg > 1:3777105/1‑51 (MQ=255) aCCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTa > 1:1995319/1‑53 (MQ=255) aCCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGc > 1:1806560/1‑71 (MQ=255) aCCGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTCTATGGCTATCAACCAGATGc > 1:1393963/1‑71 (MQ=255) ccGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCGGAAAACGGAACGTTTGTATGGCTATCAACCAGATGcc < 1:4068070/71‑1 (MQ=255) ccGGCGTTCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGcc < 1:2894150/71‑1 (MQ=255) tGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGc < 1:470326/71‑1 (MQ=255) tGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGc < 1:1719774/71‑1 (MQ=255) | TACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATGACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGC > W3110S.gb/632951‑633084 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |