| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 682,913 | C→T | 18.5% | V301V (GTG→GTA) | hscC ← | Hsp70 family chaperone Hsc62, binds to RpoD and inhibits transcription |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 682,913 | 0 | C | T | 18.5% | 57.7 / 7.8 | 27 | V301V (GTG→GTA) | hscC | Hsp70 family chaperone Hsc62, binds to RpoD and inhibits transcription |
| Reads supporting (aligned to +/- strand): ref base C (7/15); new base T (2/3); total (9/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01 | |||||||||||
TAATTTGCCAAACAGACGCACGGCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACGCCTGTTCAATCGGCACTCGCAAGC > W3110S.gb/682847‑682983 | tAATTTGCCAAACAGACGCACGGCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCACCa < 1:2119556/71‑1 (MQ=255) aTTTGCCAAACAGACGCACGGCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCACCCAGCACCAg < 1:1989637/70‑1 (MQ=255) aTTTGCCAAACAGACGCACGGCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCACCAg < 1:1170189/70‑1 (MQ=255) aTTTGCCAAACAGACGCACGGCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCACCAg < 1:1463454/70‑1 (MQ=255) cAGACGCACGGCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGTACCAGACTGTCGAttt < 1:2155786/71‑1 (MQ=255) aCGCACGGCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGTAc > 1:3132335/1‑54 (MQ=255) gCACGGCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGAttt > 1:2203704/1‑66 (MQ=255) tCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCa > 1:1605012/1‑68 (MQ=255) cGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGTACCAGACTGTCGATTTGACTCGGCTTCAGAcg > 1:983474/1‑71 (MQ=255) gCGGCATTTGTGACGCGCCGCCAACCAGTACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCgcgc < 1:940945/71‑1 (MQ=255) gCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCTATTTGACTCGGCtt < 1:2474569/55‑1 (MQ=255) gCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCtt < 1:1827420/55‑1 (MQ=255) gCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCtt < 1:2164006/55‑1 (MQ=255) gCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCtt < 1:2120584/55‑1 (MQ=255) gCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCtt < 1:2226074/55‑1 (MQ=255) ggCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCa > 1:1126253/1‑55 (MQ=255) ggCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCaa > 1:2225276/1‑71 (MQ=255) cATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACg < 1:2569262/71‑1 (MQ=255) tttGTGACGCGCCGCCAACCAGCACCAGACTGTCg < 1:2357875/35‑1 (MQ=255) tttGTGACGCGCCGCCAACCAGCACCAGACTGTCg < 1:3746515/35‑1 (MQ=255) tttGTGACGCGCCGCCAACCAGCACCAGACTGTCg < 1:775338/35‑1 (MQ=255) tttGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACGcc < 1:375214/71‑1 (MQ=255) gtgACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACGCCTGt > 1:2961164/1‑71 (MQ=255) aCGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACGCCTGTTc > 1:1171738/1‑70 (MQ=255) ccgccAACCAGTACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACGCCTGTTCAATCgg < 1:2567894/71‑1 (MQ=255) gccAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACGCCTGTTCAATCGGCa > 1:3806383/1‑71 (MQ=255) ccAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACg < 1:1192756/55‑1 (MQ=255) cACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACGCCTGTTCAATCGGCACTCGCAAGc < 1:585096/71‑1 (MQ=255) | TAATTTGCCAAACAGACGCACGGCGATTCGCTGCACCAGCGGCATTTGTGACGCGCCGCCAACCAGCACCAGACTGTCGATTTGACTCGGCTTCAGACGCGCATCGCGCAACGCCTGTTCAATCGGCACTCGCAAGC > W3110S.gb/682847‑682983 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |