| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 686,526 | A→G | 9.6% | I189T (ATC→ACC) | gltJ ← | glutamate and aspartate transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 686,526 | 0 | A | G | 9.6% | 109.9 / 3.3 | 43 | I189T (ATC→ACC) | gltJ | glutamate and aspartate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (13/25); new base G (2/2); total (15/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACGACTCCCAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCG > W3110S.gb/686457‑686596 | aaCGACTCCCAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGa > 1:3098434/1‑70 (MQ=255) cGACTCCCAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAg > 1:1087379/1‑71 (MQ=255) cGACTCCCAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAg > 1:728130/1‑71 (MQ=255) cAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGc < 1:3578638/71‑1 (MQ=255) cAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGc < 1:2413553/71‑1 (MQ=255) cAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGc < 1:915338/71‑1 (MQ=255) cAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGc < 1:1431719/71‑1 (MQ=255) aGGCATGGGCTGAGTAATCTAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCg < 1:3774345/71‑1 (MQ=255) ggCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGTTAGTCGAGGc < 1:1624740/69‑1 (MQ=255) gCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAgg < 1:1222474/67‑1 (MQ=255) cATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCg < 1:2305470/68‑1 (MQ=255) ggCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCg < 1:3423506/70‑1 (MQ=255) ggCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCg > 1:3085275/1‑70 (MQ=255) gTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGCCGGAGtttt > 1:61130/1‑71 (MQ=255) tACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcat < 1:2035903/70‑1 (MQ=255) tACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcat > 1:13223/1‑70 (MQ=255) tACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcat > 1:3209850/1‑70 (MQ=255) aCCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc < 1:598773/70‑1 (MQ=255) aCCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc < 1:1970591/70‑1 (MQ=255) aCCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc < 1:3341187/70‑1 (MQ=255) tGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc < 1:3724307/63‑1 (MQ=255) tGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc < 1:2434993/63‑1 (MQ=255) tGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc < 1:34082/63‑1 (MQ=255) tGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc < 1:3151563/63‑1 (MQ=255) gccATATCCACCAGACCGGTAGTAGAGGCGATGGCGGAGttt > 1:1048803/1‑42 (MQ=38) gccATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGt > 1:2008467/1‑40 (MQ=255) gccATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc > 1:2757466/1‑54 (MQ=255) gccATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCAt > 1:3224135/1‑71 (MQ=255) gccATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCAt > 1:3873259/1‑71 (MQ=255) ccATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATc < 1:2488558/71‑1 (MQ=255) tatCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCAcc < 1:1703542/44‑1 (MQ=255) tCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtca > 1:2130216/1‑49 (MQ=255) tCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc > 1:1834182/1‑48 (MQ=255) tCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggcgg > 1:2125563/1‑71 (MQ=255) ccaGACCGATAGTCGAGGCGATGGCGGAGTTTTTc < 1:2787015/35‑1 (MQ=255) ccaGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc < 1:2488087/47‑1 (MQ=255) ccaGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc < 1:2861330/47‑1 (MQ=255) caGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc > 1:3038552/1‑43 (MQ=255) aGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGAt < 1:357717/71‑1 (MQ=255) aGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGAt < 1:272431/71‑1 (MQ=255) aGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGAt < 1:573374/71‑1 (MQ=255) aGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGAt < 1:1934036/71‑1 (MQ=255) aGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGAt < 1:715709/71‑1 (MQ=255) gACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtca < 1:466304/42‑1 (MQ=255) aTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAAcgcg < 1:3459623/71‑1 (MQ=255) | AACGACTCCCAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCG > W3110S.gb/686457‑686596 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |