| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 690,095 | A→G | 47.1% | D403D (GAT→GAC) | lnt ← | apolipoprotein N‑acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 690,095 | 0 | A | G | 47.1% | ‑1.8 / 46.7 | 34 | D403D (GAT→GAC) | lnt | apolipoprotein N‑acyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (6/12); new base G (8/8); total (14/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
GCCATGGACCAATAGATTTACCAAACCACGCATCGTTGGAGATAGTCAGCAGATAGTCGGTATCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATGATCTCGTAGCAAATAGCCGCAGTAAGCTC > W3110S.gb/690034‑690163 | gCCATGGACCAATAGATTTACCAAACCACGCATCGTTGGAGATAGTCAGCAGATAGTCGGTGTCCGGGCg > 1:1350057/1‑70 (MQ=255) gACCAATAGATTTACGAAACCACGCATCGTTGGAGATAGTCAGCAGATAGTCGGTATCCGGGCGGAAGTTa > 1:3539825/1‑71 (MQ=255) gACCAATAGATTTACCAAACCACGCATCGTTGGAGATAGTCAGCAGATAGTCGGTATCCGGGCGGAAGTTa > 1:3631908/1‑71 (MQ=255) gACCAATAGATTTACCAAACCACGCATCGTTGGAGATAGTCAGCAGATAGTCGGTATCAGGGCGGAAGTTa > 1:3143508/1‑71 (MQ=255) aTAGATTTACCAAACCACGCATCGTTGGAGATAGTCAGCAGATAGTCGGTATCCGGGCGGAAGTTATcgcg < 1:375419/71‑1 (MQ=255) aTAGATTTACCAAACCACGCATCGTTGGAGATAGTCAGCAGATAGTCGGTATCCGGGCGGAAGTTATcgcg < 1:21484/71‑1 (MQ=255) tAGATTTACCAAACCACGCATCGTTGGAGATAGTCAGCAGATAGTCGGTGTCCGGGCGGAAGTTATCgcgc < 1:2426452/71‑1 (MQ=255) aTTTACCAAACCACGCATCGTTGGAGATAGTCAGCAGATAGTCGGTGTCCGGGCGGAAGTTATCgcgc < 1:2235084/68‑1 (MQ=255) ttACCAAACCACGCATCGTTGGAGATAGTCAGCAGATAGTCGGTGTCCGGGCGGAAGTTATCGCGCAc > 1:3117198/1‑68 (MQ=255) ttACCAAACCACGCATCGTTGGAGATAGTCAGCAGATAGTCGGTGTCCGGGCGGAAGTTATCGCGCAc > 1:3857927/1‑68 (MQ=255) cATCGTTGGAGATAGTCAGCAGATAGTCGGTGTCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATg < 1:2579453/71‑1 (MQ=255) aTCGTTGGAGATAGTCAGCAGATAGTCGGTATCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATg < 1:851623/70‑1 (MQ=255) cGTTGGAGATACTCAGCAGATAGTCGGTATCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATg < 1:1883575/68‑1 (MQ=255) ttGGAGATAGTCAGCAGATAGTCGGTATCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATGAtctc < 1:3856738/71‑1 (MQ=255) gaTAGTCAGCAGATAGTCGGTGTCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATGAt > 1:2464141/1‑63 (MQ=255) gaTAGTCAGCAGATAGTCGGTGTCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATGAt > 1:2850470/1‑63 (MQ=255) gaTAGTCAGCAGATAGTCGGTGTCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATGAt > 1:505880/1‑63 (MQ=255) gaTAGTCAGCAGATAGTCGGTGTCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATGAt > 1:3939987/1‑63 (MQ=255) aTAGTCAGCAGATAGTCGGTGTCCGGGCGGAAGTTATCGCGCa < 1:2164113/43‑1 (MQ=255) aTAGTCAGCAGATAGTCGGTGTCCGGGCGGAAGTTATCGCGCa < 1:476015/43‑1 (MQ=255) aTAGTCAGCAGATAGTCGGTGTCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATGATCTCGTAGCa < 1:2389998/71‑1 (MQ=255) aTAGTCAGCAGATAGTCGGTATCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATGATCTCGTAGc > 1:3208880/1‑70 (MQ=255) aTAGTCAGCAGATAGTCGGTATCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATGATCTCGTAGCa < 1:3570688/71‑1 (MQ=255) aTAGTCAGCAGATAGTCGGTATCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATGATCTCGTAGCa < 1:4084558/71‑1 (MQ=255) tAGTCAGCAGATAGTCGGTATCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATGATCTCGTAGCa < 1:1935991/70‑1 (MQ=255) aGTCAGCAGATAGTCGGTGTCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATGATCTCGTAGCaaa > 1:3826225/1‑71 (MQ=255) tCAGCAGATAGTCGGTATCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATGATCTCGTAGCa < 1:222491/67‑1 (MQ=255) agcagATAGTCGGTATCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATGATCTCGTAGCAAATAGc > 1:2191390/1‑71 (MQ=255) agcagATAGTCGGTATCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATGATCTCGTAGCAAATAGc > 1:1611137/1‑71 (MQ=255) gTCGGTGTCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATGATCTCGTAGCAAATAGCCGCAGTa < 1:1295379/70‑1 (MQ=255) gTCGGTGTCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATGATCTCGTAGCAAATAGCCGCAGTa < 1:2665795/70‑1 (MQ=255) gTCGGTATCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATg < 1:611010/46‑1 (MQ=255) gTCGGTATCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATGATCTCGTAGCAAATAGCCGCAGTa < 1:1476868/70‑1 (MQ=255) gTATCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATGATCTCGTAGCAAATAGCCGCAGTAAGCTc < 1:3752148/71‑1 (MQ=255) | GCCATGGACCAATAGATTTACCAAACCACGCATCGTTGGAGATAGTCAGCAGATAGTCGGTATCCGGGCGGAAGTTATCGCGCACTTGCTCGCCGAGAATGATCTCGTAGCAAATAGCCGCAGTAAGCTC > W3110S.gb/690034‑690163 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |