| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 707,271 | C→T | 16.1% | H253Y (CAC→TAC) | glnS → | glutamyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 707,271 | 0 | C | T | 16.1% | 75.6 / 7.5 | 31 | H253Y (CAC→TAC) | glnS | glutamyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base C (15/11); new base T (2/3); total (17/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01 | |||||||||||
CGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGA > W3110S.gb/707206‑707337 | cGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTTACCcg > 1:2582194/1‑71 (MQ=255) tGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCgcgc > 1:2460343/1‑70 (MQ=255) gAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCgcgc > 1:2015777/1‑69 (MQ=255) ccAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTAt < 1:2247576/70‑1 (MQ=255) cAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTtct < 1:3446587/71‑1 (MQ=255) tcTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAg < 1:1744271/52‑1 (MQ=255) tGTACGACTGGGTACTGGACAACATCACGATTCCTGTTTACCCGCGCCAGTATGAGTTCTCGCGCCTGAAt > 1:2745979/1‑71 (MQ=255) tACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCC‑CGCCAGTATGAGTTCTCGCGCCTGAATCTg < 1:1568536/71‑1 (MQ=255) cGACTGGGTACTGGACAACATCACGATTCCTGTTTACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTgg < 1:2795622/71‑1 (MQ=255) cGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTCTGAGTTCTCGCGCCTGAATCTgg < 1:1776912/71‑1 (MQ=255) cGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTg > 1:1501932/1‑70 (MQ=255) cGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTg < 1:3961870/70‑1 (MQ=255) cTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCt < 1:926077/59‑1 (MQ=255) gACAACATCACGATTCCTGTTTACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTg < 1:2195495/69‑1 (MQ=255) aacaTCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTc > 1:257712/1‑71 (MQ=255) tCACGATTCCTGTTCACCCGCGCCAGTATGAGTtct < 1:982708/36‑1 (MQ=255) cACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGaa > 1:1525600/1‑52 (MQ=255) cACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGaa > 1:2138853/1‑52 (MQ=255) cACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGaa > 1:1807399/1‑52 (MQ=255) aCGATTCCTGTTCACCCGCGCCAGTATGAGTTCTcg > 1:2475251/1‑36 (MQ=255) aCGATTCCTGTTCACCCGCGCCAGTATGAGTTCTcg > 1:2783769/1‑36 (MQ=255) aCGATTCCTGTTCACCCGCGCCAGTATGAGTTCTcg > 1:3953978/1‑36 (MQ=255) aCGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTg > 1:357599/1‑48 (MQ=255) aCGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCg > 1:2768422/1‑71 (MQ=255) aCGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCg > 1:2174777/1‑71 (MQ=255) aCGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCg > 1:1895360/1‑71 (MQ=255) aCGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCg > 1:928023/1‑71 (MQ=255) cTGTTTACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTg < 1:2441523/71‑1 (MQ=255) cTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTg < 1:3802789/71‑1 (MQ=255) ttCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGa < 1:2314447/69‑1 (MQ=255) ttCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGa < 1:2810635/69‑1 (MQ=255) | CGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGA > W3110S.gb/707206‑707337 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |