| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 712,019 | G→A | 13.5% | intergenic (‑132/+8) | fldA ← / ← ybfE | flavodoxin 1/LexA regulated protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 712,019 | 0 | G | A | 13.5% | 244.0 / 25.0 | 89 | intergenic (‑132/+8) | fldA/ybfE | flavodoxin 1/LexA regulated protein |
| Reads supporting (aligned to +/- strand): ref base G (48/29); new base A (9/3); total (57/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
AATCATAGCAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTC > W3110S.gb/711950‑712089 | aaTCATAGCAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGt > 1:427938/1‑71 (MQ=255)aaTCATAGCAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGt > 1:3822656/1‑71 (MQ=255)aaTCATAGCAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGt > 1:2055893/1‑71 (MQ=255)aaTCATAGCAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGt > 1:1945657/1‑71 (MQ=255) aTAGCAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGa < 1:130661/70‑1 (MQ=255) cAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAAc > 1:345/1‑70 (MQ=255) cAGTCTTGCGGAATCAGCAAACGAGCAGGACTGAACACTGTGCTACATGAAAGTGGAAATTTAAAc < 1:425265/66‑1 (MQ=255) cTTGCGGAATCAGCAAACGAGCAGGACTGCCCACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCt > 1:2340916/1‑70 (MQ=255) cTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCt > 1:1122958/1‑70 (MQ=255) cTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCt > 1:2320142/1‑70 (MQ=255) ttGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGa > 1:4064337/1‑71 (MQ=255) ttGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGa > 1:2962020/1‑71 (MQ=255) ttGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGa > 1:1720869/1‑71 (MQ=255) aaTCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAAc < 1:1007968/55‑1 (MQ=255) aaTCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAAc < 1:2545510/55‑1 (MQ=255) aaTCAGCAAACGAGCAGGACTGCACACTGTGCTACATCAAAGTGGAAATTTAAAc < 1:1073594/55‑1 (MQ=255) aGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAAc < 1:678204/51‑1 (MQ=255) aGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAAc < 1:3860755/51‑1 (MQ=255) aGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAAc < 1:2029511/51‑1 (MQ=255) aGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAAGGATGCCCTGa > 1:1312829/1‑61 (MQ=255) aGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAgcg > 1:2826535/1‑71 (MQ=255) aGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAgcg > 1:4032789/1‑71 (MQ=255) aGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAgcg > 1:2069846/1‑71 (MQ=255) cAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGa > 1:1497118/1‑51 (MQ=255) cAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGa > 1:2228720/1‑51 (MQ=255) cAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGa > 1:2013219/1‑51 (MQ=255) cAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGa > 1:4003359/1‑51 (MQ=255) cAAACGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGa > 1:625150/1‑51 (MQ=255) aaaCGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGa > 1:769418/1‑50 (MQ=255) aaaCGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATATAAACGa > 1:234427/1‑50 (MQ=255) aaaCGAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGcgccg > 1:2300083/1‑71 (MQ=255) gAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAg < 1:3130819/71‑1 (MQ=255) gAGCAGGACTGCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAg > 1:1050948/1‑71 (MQ=255) gCAGGACTGAACACTGTGCTACATGAAAATGGAAATTTAAAc < 1:3560551/42‑1 (MQ=38) gACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTtgctg < 1:1107388/71‑1 (MQ=255) gACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTtgctg < 1:2959504/71‑1 (MQ=255) aCTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTtgctg < 1:1865321/70‑1 (MQ=255) aCTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTtgctg > 1:1373750/1‑70 (MQ=255) aCTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTtgctg > 1:2682189/1‑70 (MQ=255) aCTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTtgctg > 1:2925060/1‑70 (MQ=255) cTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAg > 1:459380/1‑63 (MQ=255) cTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAg > 1:454453/1‑63 (MQ=255) cTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa > 1:3804102/1‑71 (MQ=255) cTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa > 1:1051207/1‑71 (MQ=255) tGCACACTGTGCTACATGAAAGTGGAAATTTAAAc < 1:1101501/35‑1 (MQ=255) tGCACACTGTGCTACATGAAAGTGGAAATTTAAAc < 1:1339584/35‑1 (MQ=255) tGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGc < 1:1419033/51‑1 (MQ=255) tGCACACTGTGCTACATGAAAATGGAAATTTAAAc < 1:804239/35‑1 (MQ=255) gCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAgcgc > 1:1361836/1‑55 (MQ=255) gCACACTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAgcgc > 1:3131324/1‑55 (MQ=255) cacaCTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCAtt > 1:3819727/1‑70 (MQ=255) cacaCTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCAtt > 1:3491659/1‑70 (MQ=255) cacaCTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCAtt > 1:3891741/1‑70 (MQ=255) cacaCTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCAtt > 1:3000496/1‑70 (MQ=255) cacaCTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCAtt > 1:1729204/1‑70 (MQ=255) cacaCTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCAtt > 1:2976009/1‑70 (MQ=255) cacaCTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCAtt > 1:2606526/1‑70 (MQ=255) cacaCTGTGCTACATGAAAATGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCAtt < 1:3490078/70‑1 (MQ=255) acacTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTaa < 1:1004470/71‑1 (MQ=255) cacTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGa > 1:3169873/1‑41 (MQ=255) cacTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGa > 1:3016565/1‑41 (MQ=255) cacTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAAc < 1:4075792/71‑1 (MQ=255) cTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAt > 1:3639471/1‑71 (MQ=255) tgtgCTACATGAAAGTGGAAATTTAAACGATGCCCt < 1:2265504/36‑1 (MQ=255) tgtgCTACATGAAAGTGGAAATTTAAACGATGCCCt < 1:881562/36‑1 (MQ=255) tgtgCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtc > 1:988611/1‑71 (MQ=255) gtgCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtct > 1:3568737/1‑71 (MQ=255) gtgCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtct > 1:3731840/1‑71 (MQ=255) gagCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtct > 1:2986031/3‑71 (MQ=255) tgCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTaa > 1:3977872/1‑65 (MQ=255) tgCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTaa > 1:2274232/1‑65 (MQ=255) tgCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGAAGCGCCGCCAGTTGCTGCATTaa > 1:1085348/1‑65 (MQ=255) cTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATctct < 1:1259508/70‑1 (MQ=255) tACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCgccgcc < 1:342261/48‑1 (MQ=255) tACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCgccgcc < 1:3098906/48‑1 (MQ=255) tACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTtgc < 1:1667310/54‑1 (MQ=255) cATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCg > 1:866969/1‑70 (MQ=255) cATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGa > 1:2143018/1‑71 (MQ=255) cATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGa > 1:467362/1‑71 (MQ=255) aTGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGAt < 1:272125/71‑1 (MQ=255) tGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCg < 1:2155212/68‑1 (MQ=255) tGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCg < 1:4000926/68‑1 (MQ=255) aaaGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGAt > 1:3031740/1‑68 (MQ=255) aaaGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGAt > 1:2379994/1‑68 (MQ=255) aaaGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCa < 1:4084905/70‑1 (MQ=255) aaaGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCa < 1:2838742/70‑1 (MQ=255) aaGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGt > 1:3134269/1‑71 (MQ=255) aaGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGt < 1:837839/71‑1 (MQ=255) aaGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGt > 1:3224601/1‑71 (MQ=255) gTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTc > 1:1600499/1‑71 (MQ=255) | AATCATAGCAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTC > W3110S.gb/711950‑712089 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |