| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 726,253 | A→G | 13.6% | R402R (CGT→CGC) | kdpB ← | potassium translocating ATPase, subunit B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 726,253 | 0 | A | G | 13.6% | 107.8 / 10.1 | 44 | R402R (CGT→CGC) | kdpB | potassium translocating ATPase, subunit B |
| Reads supporting (aligned to +/- strand): ref base A (21/17); new base G (3/3); total (24/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
ACGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGC > W3110S.gb/726184‑726322 | aCGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGcgac < 1:2677725/71‑1 (MQ=255)aCGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGcgac < 1:2348342/71‑1 (MQ=255) cgcgCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGcgacg < 1:3277577/71‑1 (MQ=255) gcgcAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGcgacg > 1:1415457/1‑70 (MQ=255) tGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATc > 1:3737354/1‑71 (MQ=255) tGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATc > 1:3414943/1‑71 (MQ=255) cGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGGCGAATGGCATCGa > 1:1052319/1‑69 (MQ=255) cGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGGCGAATGGCATCGa > 1:2093296/1‑69 (MQ=255) cGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGaa > 1:2336142/1‑60 (MQ=255) gACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGa > 1:1637829/1‑68 (MQ=255) cTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGcga > 1:2237713/1‑53 (MQ=255) tGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCtt < 1:1138293/70‑1 (MQ=255) tCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACg < 1:999565/71‑1 (MQ=255) tCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACg < 1:3989250/71‑1 (MQ=255) aaCATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATgg > 1:1996515/1‑50 (MQ=255) aaCATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATgg > 1:3626864/1‑50 (MQ=255) aaCATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATgg > 1:1307117/1‑50 (MQ=255) aaCATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATgg > 1:3021232/1‑50 (MQ=255) aaCATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATgg > 1:2898862/1‑50 (MQ=255) aaCATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGa < 1:346873/71‑1 (MQ=255) ggTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGa > 1:2911739/1‑50 (MQ=255) gTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCg < 1:3287304/71‑1 (MQ=255) tAGGGAAGTGACCACCGTTAGCCTCAACATGGcgacg < 1:1762673/37‑1 (MQ=255) aaGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTc > 1:3190526/1‑71 (MQ=255) gTGACCACCGTTAGCCTCAACATGTCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGa < 1:2741017/71‑1 (MQ=255) gTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATg < 1:3576491/61‑1 (MQ=255) gTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATg < 1:1323233/61‑1 (MQ=255) gTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGa < 1:613558/71‑1 (MQ=255) ccaccGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGtt < 1:2486213/71‑1 (MQ=255) ccaccGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGtt < 1:277353/71‑1 (MQ=255) accGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCtt < 1:1732334/44‑1 (MQ=255) ccGTTAGCCTCAACATGGCGGCGAATGGCATCGTCAGAACCTTTACGGATCATGCGGTTGTc < 1:4081203/62‑1 (MQ=255) ccGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTc < 1:565867/62‑1 (MQ=255) cGTTAGCCTCAACATGGCGGCGAATGGCATCGACAGAACCTTTa > 1:2366258/1‑44 (MQ=255) tAGCCTCAACATGGCGACGAATGGCATCGACAGAAcc > 1:209904/1‑37 (MQ=255) tAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCg > 1:2638691/1‑71 (MQ=255) aGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCa > 1:2456932/1‑47 (MQ=255) cTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCTGTTGTCGATGTTGATCCCGCTCa < 1:1034346/71‑1 (MQ=255) cTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCa < 1:3602889/71‑1 (MQ=255) cTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCa < 1:3376828/71‑1 (MQ=255) aaCATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGa > 1:2945352/1‑53 (MQ=255) aaCATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATc > 1:3932642/1‑61 (MQ=255) aaCATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATc > 1:318895/1‑61 (MQ=255) aaCATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATc > 1:60240/1‑61 (MQ=255) aaCATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATc > 1:1856830/1‑61 (MQ=255) aaCATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATc < 1:1819527/70‑1 (MQ=255) cATGGCGACGAATGGCATCGACAGAACCTTTACGGATCAt < 1:1593566/40‑1 (MQ=255) aTGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGc < 1:391128/71‑1 (MQ=255) ggCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCtt > 1:3289703/1‑71 (MQ=255) gCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCttt > 1:524207/1‑71 (MQ=255) cgacgaATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTg < 1:3438763/71‑1 (MQ=255) gacgaATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTgc > 1:4004379/1‑71 (MQ=255) | ACGCGCAACCTGATCGACTTTTTGATCAACATCGGTAGGGAAGTGACCACCGTTAGCCTCAACATGGCGACGAATGGCATCGACAGAACCTTTACGGATCATGCGGTTGTCGATGTTGATCCCGCTCATCCGGCTTTGC > W3110S.gb/726184‑726322 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |