| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 767,363 | T→C | 26.5% | L320L (TTG→CTG) | mngA → | fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzyme IIABC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 767,363 | 0 | T | C | 26.5% | 50.9 / 20.1 | 34 | L320L (TTG→CTG) | mngA | fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzyme IIABC components |
| Reads supporting (aligned to +/- strand): ref base T (11/14); new base C (5/4); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01 | |||||||||||
AACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAG > W3110S.gb/767295‑767429 | aaCCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTATTTTGCCGTACCGCTg < 1:4060961/71‑1 (MQ=255) tCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGtt < 1:304485/55‑1 (MQ=255) tCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGtt < 1:2068351/55‑1 (MQ=255) tCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGtt < 1:3442826/55‑1 (MQ=255) tCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGtt < 1:587284/55‑1 (MQ=255) tCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGTGc < 1:1716592/71‑1 (MQ=255) aaaaCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGCTGATTGTCGCGGGGGGCAc > 1:2925855/1‑71 (MQ=255) aaaaCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGCTGATTGTCGCGGGGGGCAc > 1:1424392/1‑71 (MQ=255) aaaaCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGCTGATTGTCGCGGGGGGCAc > 1:1630206/1‑71 (MQ=255) aaaaCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGCTGATTGTCGCGGGGGGCAc > 1:165915/1‑71 (MQ=255) gAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCgg > 1:1969867/1‑71 (MQ=255) gAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCgg > 1:513838/1‑71 (MQ=255) cAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTcgc > 1:3438484/1‑71 (MQ=255) ggCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCgg < 1:3102409/71‑1 (MQ=255) ggCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCgg < 1:3285566/71‑1 (MQ=255) aCTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTAt < 1:3799896/71‑1 (MQ=255) tGTTGAGCGGAATCTCTTTTGCCGTACCGCTGATTGTCGCGGGGGGCACGGt < 1:607929/52‑1 (MQ=255) gTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTAc > 1:1087302/1‑71 (MQ=255) gTTGAGCGGAATCTCTTTTGCCGTACCGCTGATTGTCGCGGGGGGCACGGt < 1:2826912/51‑1 (MQ=255) ttGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCgggggg < 1:270963/44‑1 (MQ=255) ttGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCgggggg < 1:3317108/44‑1 (MQ=255) ttGAGCGGAATCTCTTTTGCCGTACCGCTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACt < 1:3723704/71‑1 (MQ=255) tGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCCCgggggg < 1:3026301/43‑1 (MQ=255) ggAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCa < 1:665548/71‑1 (MQ=255) aTCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCaaa > 1:2628412/1‑70 (MQ=255) aTCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAAt > 1:929934/1‑71 (MQ=255) aTCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAAt > 1:757047/1‑71 (MQ=255) ttttGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCt < 1:3768592/68‑1 (MQ=255) ttttGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCt < 1:493240/68‑1 (MQ=255) ttGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCgg > 1:3655236/1‑48 (MQ=255) ttGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCgg > 1:2390219/1‑48 (MQ=255) ttGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCgg > 1:192053/1‑48 (MQ=255) ttGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCggg > 1:3371050/1‑71 (MQ=255) cGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTAc < 1:557026/71‑1 (MQ=255) gTACCGCTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTAc > 1:1227969/1‑70 (MQ=255) ccGTTGATTGTCGCGGGGGGCAGGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAg < 1:1322928/70‑1 (MQ=255) ccGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAg < 1:2102014/70‑1 (MQ=255) ccGTTGATTGTCGCGGGGGGCACGGTGCTGGAGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAg < 1:3668902/70‑1 (MQ=255) | AACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAG > W3110S.gb/767295‑767429 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |