| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 809,043 | T→C | 13.0% | I213V (ATT→GTT) | bioA ← | 7,8‑diaminopelargonic acid synthase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 809,043 | 0 | T | C | 13.0% | 116.7 / 10.1 | 46 | I213V (ATT→GTT) | bioA | 7,8‑diaminopelargonic acid synthase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (24/16); new base C (2/4); total (26/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01 | |||||||||||
TTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCACCGCCGCGATTTCATGACGATGCGCCGCCATCAGGCGGGCAAAGCCCACCA > W3110S.gb/808980‑809108 | ttttGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTc > 1:3452447/1‑70 (MQ=255)ttttGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTc > 1:191550/1‑70 (MQ=255)ttttGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTc > 1:4004673/1‑70 (MQ=255)ttttGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCa < 1:1510808/71‑1 (MQ=255)ttttGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAACCGGCTc > 1:3606619/1‑70 (MQ=255) cGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGAt < 1:1042654/71‑1 (MQ=255) cGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGAt < 1:384808/71‑1 (MQ=255) gATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCa > 1:1322561/1‑71 (MQ=255) gATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCa > 1:3045451/1‑71 (MQ=255) tCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCAccg > 1:3854197/1‑71 (MQ=255) tCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCAcc > 1:3760793/1‑70 (MQ=255) tCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAACCGGCTCAATGATCAccg > 1:2335891/1‑71 (MQ=255) ttAACCATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCACcgccgc < 1:1055558/70‑1 (MQ=255) ttAACCATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCACcgccgc > 1:2412359/1‑70 (MQ=255) tAACCATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCACCGCCGCGa > 1:1969089/1‑71 (MQ=255) ccATTCCGGATTGTACATGCGCATCCCGCCTGCGCCCTGGACAACCGGCTCAATGATCACCGCCGCGAttt < 1:2045376/71‑1 (MQ=255) ccATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCACCGCCTCGAttt < 1:528184/71‑1 (MQ=255) ccATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAACCGGCTCAATGATCACCGCCGCGAttt < 1:1008590/71‑1 (MQ=255) ccGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCACCGCCGCGATTTCATg > 1:623425/1‑70 (MQ=255) ccGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCACCGCCGCGATTTCATGa > 1:2600787/1‑71 (MQ=255) ccGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCACCGCCGCGATTTCATGa > 1:3001068/1‑71 (MQ=255) ccGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCACCGCCGCGATTTCATGa > 1:2854215/1‑71 (MQ=255) cGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCACCGCCGCGATTTCATGAc < 1:1320577/71‑1 (MQ=255) cGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCACCGCCGCGATTTCATGAc < 1:2664342/71‑1 (MQ=255) cGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCACCGCCGCGATTTCATGAc < 1:3973240/71‑1 (MQ=255) cGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCACCGCCGCGATTTCATGAc < 1:1601465/71‑1 (MQ=255) ggATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCACCGCCGCGATTTCATGAc < 1:193385/70‑1 (MQ=255) cATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCACCGCCGCGATTTCATGAc < 1:1843379/62‑1 (MQ=255) gcgcATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCACCGCCGCGATTTCATGACGATGCgccgcc > 1:2266210/1‑70 (MQ=255) cgcATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCACCGCCGCGATTTCATGACGa > 1:1953269/1‑60 (MQ=255) ccGCCTGCGCCCTGGACAATCGGCTCAATGATCAc > 1:3036071/1‑35 (MQ=255) ccGCCTGCGCCCTGGACAATCGGCTCAATGATCAc > 1:612748/1‑35 (MQ=255) ccGCCTGCGCCCTGGACAATCGGCTCAATGATCACCGCCGCGATTTCa > 1:3261802/1‑48 (MQ=255) cTGCGCCCTGGACAACCGGCTCAATGATCACCGCCTCGATTTCATGACGATGCGCCGCCATCAGGCGGGCa < 1:2320059/71‑1 (MQ=255) cTGCGCCCTGGACAACCGGCTCAATGATCACCGCCGCGATTTCATGACGATGCGCCGCCATCAGGCGGCCa < 1:1464848/71‑1 (MQ=255) tGCGCCCTGGACAATCGGCTCAATGATCACCGCCGCGATTTCATTACGATGCGCCGCCATCAGGCGGGCa > 1:3929371/1‑70 (MQ=255) gcgcCCTGGACAATCGGCTCAATGATCACCGCCGCGa > 1:1256247/1‑37 (MQ=255) gcgcCCTGGACAATCGGCTCAATGATCACCGCCGCGAttt < 1:1708254/40‑1 (MQ=255) cgcCCTGGACAATCGGCTCAATGATCACCGCCGCGATTTCATGACGATGCGCCGCCATCAGGCGGGCAAAg > 1:2839776/1‑71 (MQ=255) gACAATCGGCTCAATGATCACCGCCGCGATTTCATGACGATGCGCCGCCATCAGGCGGGCAAAGCccacca > 1:2948519/1‑71 (MQ=255) gACAATCGGCTCAATGATCACCGCCGCGATTTCATGACGATGCGCCGCCATCAGGCGGGCAAAGCccacca > 1:474406/1‑71 (MQ=255) gACAATCGGCTCAATGATCACCGCCGCGATTTCATGACGATGCGCCGCCATCAGGCCGGCAAAGCccac < 1:232698/69‑1 (MQ=255) gACAATCGGCTCAATGATCACCGCCGCGATTTCATGACGATGCGCCGCCATAAGGCGGGCAAAGCccacca > 1:24942/1‑71 (MQ=255) aTCGGCTCAATGATCACCGCCGCGATTTCATGACGATgc < 1:2143463/39‑1 (MQ=255) aTCGGCTCAATGATCACCGCCGCGATTTCATGACGATgc < 1:762648/39‑1 (MQ=255) aTCGGCTCAATGATCACCGCCGCGATTTCATGAAGATGCGCCGCCATCa < 1:444095/49‑1 (MQ=255) | TTTTGCGGATTCGTTTTAACCATTCCGGATGGTACATGCGCATCCCGCCTGCGCCCTGGACAATCGGCTCAATGATCACCGCCGCGATTTCATGACGATGCGCCGCCATCAGGCGGGCAAAGCCCACCA > W3110S.gb/808980‑809108 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |