| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 823,380 | G→A | 41.2% | P261L (CCG→CTG) | ybhO ← | cardiolipin synthase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 823,380 | 0 | G | A | 41.2% | 18.5 / 36.5 | 34 | P261L (CCG→CTG) | ybhO | cardiolipin synthase 2 |
| Reads supporting (aligned to +/- strand): ref base G (7/13); new base A (6/8); total (13/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01 | |||||||||||
CTCAAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGC > W3110S.gb/823313‑823442 | cTCAAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGc > 1:1503882/1‑70 (MQ=255) aaaCCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATcc < 1:317065/71‑1 (MQ=255) cgccTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGa < 1:3253767/71‑1 (MQ=255) cgccTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCAGCATATCCGGTTCGCCCTg < 1:2987529/70‑1 (MQ=255) cgccTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCAGCATATCCGGTTCGCCCTGa < 1:771343/71‑1 (MQ=255) gccTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCAGCATATCCGGTTCGCCCTGaa > 1:4010104/1‑71 (MQ=255) aGTTATACAGCAAGCGCGCACCGACTCTGACAATCAGCATATCCGGTTCGCCCTGAATGATCAGTTTGATc > 1:121968/1‑71 (MQ=255) tataCAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCg < 1:1024972/70‑1 (MQ=255) tataCAGCAAGCGCGCACCGACTCTGACAATCAGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCg > 1:3285093/1‑70 (MQ=255) ataCAGCAAGCGCGCACCGACTCTGACAATCGGCa > 1:157610/1‑35 (MQ=255) ataCAGCAAGCGCGCACCGACTCTGACAATCGGCa > 1:3881248/1‑35 (MQ=255) ataCAGCAAGCGCGCACCGACTCTGACAATCAGCa > 1:3809150/1‑35 (MQ=255) taCAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTg < 1:3580936/63‑1 (MQ=255) taCAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTg < 1:4033044/63‑1 (MQ=255) gCAAGCGCGCACCGACTCTGACAATCAGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCg < 1:764051/64‑1 (MQ=255) gCAAGCGCGCACCGACTATGACAATCAGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCg < 1:27799/64‑1 (MQ=255) aaGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCcgcg > 1:3018361/1‑71 (MQ=255) aaGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCcgcg > 1:1548518/1‑71 (MQ=255) aGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGaa > 1:339924/1‑45 (MQ=255) gcgcgcACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGa < 1:725142/56‑1 (MQ=255) gcgcgcACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGcc < 1:2276860/71‑1 (MQ=255) gcgcgcACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGcc < 1:102798/71‑1 (MQ=255) gcgcgcACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGcc < 1:107628/71‑1 (MQ=255) gcgcgcACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGcc < 1:1647229/71‑1 (MQ=255) gcgcgcACCGACTCTGACAATCAGCATATCCGGTTCGCCCTGAATGATCAGTTTGa < 1:2546259/56‑1 (MQ=255) gcgcgcACCGACTCTGACAATCAGCATATCCGGTTCGCCCTGAATGATCAGTTTGa < 1:2065083/56‑1 (MQ=255) gcgcgcACCGACTCTGACAATCAGCATATCCGGTTCGCCCTGAATGATCAGTTTGa < 1:4011769/56‑1 (MQ=255) gcgcgcACCGACTCTGACAATCAGCATATCCGGTTCGCCCTGAATGATCAGTTTGa < 1:1174020/56‑1 (MQ=255) ccGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATg < 1:2537046/39‑1 (MQ=255) ccGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATg < 1:2270419/39‑1 (MQ=255) cGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCt > 1:606050/1‑71 (MQ=255) gACTCTGACAATCAGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGc > 1:1631089/1‑69 (MQ=255) aCAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGc < 1:3637360/69‑1 (MQ=255) aCAATCAGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCa > 1:521866/1‑46 (MQ=255) | CTCAAAAACCTGAACGCCGCCTTTAACCAGATAGTTATACAGCAAGCGCGCACCGACTCTGACAATCGGCATATCCGGTTCGCCCTGAATGATCAGTTTGATCCGCACCCCGCGCCGTGCCGCTTTACGC > W3110S.gb/823313‑823442 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |