| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 847,104 | A→G | 17.2% | R146R (CGT→CGC) | glnP ← | glutamine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 847,104 | 0 | A | G | 17.2% | 63.9 / 8.2 | 29 | R146R (CGT→CGC) | glnP | glutamine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (6/18); new base G (3/2); total (9/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
GCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCC > W3110S.gb/847035‑847167 | gCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATac < 1:3519336/71‑1 (MQ=255)gCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATac < 1:3347838/71‑1 (MQ=255)gCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATac < 1:2065574/71‑1 (MQ=255) tCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGc < 1:1185064/71‑1 (MQ=255) cacaATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATacgacg > 1:755934/1‑69 (MQ=255) aaTAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGt < 1:2028307/70‑1 (MQ=255) tAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGc > 1:2635483/1‑70 (MQ=255) aaCAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAg > 1:2943578/1‑71 (MQ=255) cAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGt < 1:2320899/64‑1 (MQ=255) gCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCgg > 1:3117676/1‑70 (MQ=255) gAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTaa < 1:2460854/71‑1 (MQ=255) tgtCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATg < 1:2306782/71‑1 (MQ=255) tgtCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTaaa < 1:3922457/68‑1 (MQ=255) tgtCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTaaa < 1:2450983/68‑1 (MQ=255) tCTTTAATGCTGATGATCCACTGGTTACCCAGTGGCGGCAGCATACGACGCAGt < 1:3571513/54‑1 (MQ=255) tttAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTaa < 1:3869136/63‑1 (MQ=255) tAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACg < 1:1639829/68‑1 (MQ=255) tAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAg > 1:1583600/1‑71 (MQ=255) aTGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCg < 1:1273341/71‑1 (MQ=255) cTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGaa < 1:2666467/70‑1 (MQ=255) cTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGaa < 1:2450672/70‑1 (MQ=255) cTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGaa < 1:2082060/70‑1 (MQ=255) gatCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCg < 1:2376524/63‑1 (MQ=255) cTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTaaa < 1:3305067/46‑1 (MQ=255) aCCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTcc > 1:3837165/1‑61 (MQ=255) aCCCAGCGGCGGCAGCATGCGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGa > 1:1928398/1‑69 (MQ=255) cccAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGaaa < 1:1303659/70‑1 (MQ=255) gcggcAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAgc > 1:3224158/1‑71 (MQ=255) gcggcAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAgc > 1:555755/1‑71 (MQ=255) gcggcAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAgc > 1:74023/1‑71 (MQ=255) tACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGcc < 1:3093154/65‑1 (MQ=255) tACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGcc < 1:3577110/65‑1 (MQ=255) tACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGcc < 1:661739/65‑1 (MQ=255) acgacgCAGTGCCAGCGGTAAAATGACGTAGCGaa > 1:3018714/1‑35 (MQ=255) | GCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCC > W3110S.gb/847035‑847167 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |