| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 865,658 | A→G | 9.1% | F377L (TTT→CTT) | moeA ← | molybdopterin biosynthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 865,658 | 0 | A | G | 9.1% | 144.1 / 6.0 | 55 | F377L (TTT→CTT) | moeA | molybdopterin biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base A (25/25); new base G (2/3); total (27/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCGGCGTTGC > W3110S.gb/865590‑865723 | cACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAgg < 1:507189/71‑1 (MQ=255) cATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGc > 1:2222525/1‑70 (MQ=255) tCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTaaa > 1:321346/1‑53 (MQ=255) tCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTaaa > 1:1453106/1‑53 (MQ=255) tCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTaaa > 1:1594332/1‑53 (MQ=255) tCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTaaa > 1:2291841/1‑53 (MQ=255) aTTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCt < 1:2073135/71‑1 (MQ=255) aTTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCt < 1:3855574/71‑1 (MQ=255) ttGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCt < 1:547734/70‑1 (MQ=255) ttGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCt > 1:462370/1‑70 (MQ=255) ttGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTg < 1:1014588/71‑1 (MQ=255) ttGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTg < 1:251226/71‑1 (MQ=255) ttGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTg < 1:1421628/71‑1 (MQ=255) ttGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTg < 1:181190/71‑1 (MQ=255) gCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGAt > 1:2482083/1‑71 (MQ=255) gCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGAt > 1:1713856/1‑71 (MQ=255) gCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGAt > 1:1505966/1‑71 (MQ=255) gCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGAt > 1:54945/1‑71 (MQ=255) ccGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGAt < 1:2459220/70‑1 (MQ=255) ccGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGAt < 1:2207928/70‑1 (MQ=255) tCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTaaa < 1:1040908/47‑1 (MQ=255) cgcgTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGa > 1:3703449/1‑62 (MQ=255) cgcgTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGa > 1:3926632/1‑62 (MQ=255) cgcgTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCggtg > 1:2410678/1‑71 (MQ=255) gTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGgtc > 1:3845793/1‑71 (MQ=255) gTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGgtc > 1:121275/1‑71 (MQ=255) ccAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGa > 1:321738/1‑56 (MQ=255) ccAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGa > 1:851251/1‑56 (MQ=255) ccAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCgg > 1:2915526/1‑63 (MQ=255) ccAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCgg > 1:1619427/1‑63 (MQ=255) cACGATAAAGCAGTTTCCGAGGCTAAGGGAGCTAAATATATGTGAACCCTGa > 1:3442671/1‑52 (MQ=255) cACGATAAAGCAGTTGCCGAGGCTAAGGGAGCTAAATATATGTGAACCCTGa > 1:3727495/1‑52 (MQ=255) aCGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTc > 1:3072214/1‑71 (MQ=255) aCGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTc > 1:3808173/1‑71 (MQ=255) gATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAata < 1:1044738/35‑1 (MQ=255) gATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAata < 1:2593012/35‑1 (MQ=255) gATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAata < 1:1081504/35‑1 (MQ=255) gATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAata < 1:1175081/35‑1 (MQ=255) gATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAata < 1:3886710/35‑1 (MQ=255) tAAAGCAGTTGCCGAGGCTAAAGGAGCTAAatata > 1:1244498/1‑35 (MQ=255) tAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGa > 1:3331415/1‑40 (MQ=255) tAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGt > 1:3608480/1‑71 (MQ=255) aaaGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGAt > 1:3536956/1‑47 (MQ=255) aaaGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGAt > 1:2933528/1‑47 (MQ=255) aaaGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGAt > 1:4072177/1‑47 (MQ=255) cAGTTGCCGAGGCTAAAGTAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGc < 1:20944/70‑1 (MQ=255) cAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGc < 1:3404300/70‑1 (MQ=255) cAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGc < 1:2937705/70‑1 (MQ=255) gCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCgg > 1:1217962/1‑71 (MQ=255) gCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCg > 1:370559/1‑70 (MQ=255) ccGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCgg < 1:1847112/70‑1 (MQ=255) ccGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCGgc < 1:2762562/71‑1 (MQ=255) ccGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCGgc < 1:1638360/71‑1 (MQ=255) ccGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCGgc < 1:2247896/71‑1 (MQ=255) ccGAGGCTAAAGGAGCTAAATATATGTGAACCATGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCgg < 1:2548694/70‑1 (MQ=255) gCTAAGGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCGgcgtt < 1:1254375/69‑1 (MQ=255) gCTAAGGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCGgcgtt < 1:3878806/69‑1 (MQ=255) gCTAAGGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCGgcgtt < 1:3238468/69‑1 (MQ=255) gCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCGgcgttgc < 1:2666168/71‑1 (MQ=255) aaGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCAc > 1:2362986/1‑44 (MQ=255) | CACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGCAGTTGCCGAGGCTAAAGGAGCTAAATATATGTGAACCCTGATGTCCGGTGGTCGTCACTTCCAGTTCGCCATCGGCGTTGC > W3110S.gb/865590‑865723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |