Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	867,056	C→T	10.5%	Q23* (CAA→TAA)	iaaA →	L‑asparaginase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	867,056	0	C	T	10.5%	108.8 / 4.0	38	Q23* (CAA→TAA)	iaaA	L‑asparaginase
Reads supporting (aligned to +/- strand):  ref base C (17/17);  new base T (2/2);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

CTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGAAACCGGGCAGAAAATGCTGGAAGCG  >  W3110S.gb/866988‑867124                                                                     |                                                                     cTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCG‑AGGTGCAATTAGCCGCGCGCAGATGAGTCTCCaa                                                                    <  1:2275765/70‑1 (MQ=255)    tGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAg                                                                 >  1:2093802/1‑71 (MQ=255)      ggCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAgg                                                                <  1:3425269/70‑1 (MQ=255)      ggCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAgg                                                                <  1:2132029/70‑1 (MQ=255)      ggCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGa                                                               <  1:961745/71‑1 (MQ=255)       gCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGaa                                                              >  1:1033643/1‑71 (MQ=255)       gCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGaa                                                              >  1:3221400/1‑71 (MQ=255)       gCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGaa                                                              >  1:1552341/1‑71 (MQ=255)          aaGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAATTa                                                           <  1:3614026/71‑1 (MQ=255)          aaGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAATTa                                                           >  1:1478257/1‑71 (MQ=255)                tCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAATTACGCTAc                                                     <  1:1000045/71‑1 (MQ=255)                 cATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAATTACGCTACa                                                    >  1:3868390/1‑71 (MQ=255)                           cATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAATTACGCTACATCGAGGCGtt                                          >  1:2320723/1‑71 (MQ=255)                               gtggCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAATTACGCTACATCGAGGCGTTGTc                                       <  1:3775944/70‑1 (MQ=255)                                       ggTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAATTACGCTACATCg                                                 <  1:1630036/52‑1 (MQ=255)                                       ggTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAATTACGCTACATCg                                                 <  1:4003149/52‑1 (MQ=255)                                        gTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAATTACGCTACATCGAGGc                                             <  1:3581324/55‑1 (MQ=255)                                        gTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAATTACGCTACATCGAGGCGTTGt                                        <  1:3001182/60‑1 (MQ=255)                                        gTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAATTACGCTACATCGAGGCGTTGt                                        <  1:1614059/60‑1 (MQ=255)                                         tGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCAttgtt                             >  1:3316547/1‑70 (MQ=255)                                          gCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAATTACGCTACATCGAGGCGTTGTCt                                      >  1:3024104/1‑60 (MQ=255)                                           cAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGaa                          <  1:1104547/71‑1 (MQ=255)                                             aTTAGCCGCGCGCAGATGAGTCTGCAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGAAAc                        >  1:3922036/1‑71 (MQ=255)                                              ttAGCCGCGCGCAGATGAGTCTGCAACAGGAATTACGCTACATCg                                                 <  1:3592391/45‑1 (MQ=255)                                              ttAGCCGCGCGCAGATGAGTCTGCAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGa                           <  1:96251/67‑1 (MQ=255)                                                aGCCGCGCGCAGATGAGTCTGCAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGAAACCgg                     >  1:2485053/1‑71 (MQ=255)                                                aGCCGCGCGCAGATGAGTCTGCAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGAAACCgg                     >  1:657431/1‑71 (MQ=255)                                                    gcgcgcAGATGAGTCTGCAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGAAACCggg                    >  1:25717/1‑68 (MQ=255)                                                    gcgcgcAGATGAGTCTGCAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGAAACCggg                    >  1:1756318/1‑68 (MQ=255)                                                    gcgcgcAGATGAGTCTGCAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGAAACCGGGCAg                 <  1:1343800/71‑1 (MQ=255)                                                      gcgcAGATGAGTCTGCAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGAAACCGGGCAGaa               <  1:860646/71‑1 (MQ=255)                                                       cgcAGATGAGTCTGTAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGAAACCGGGCAGaaa              <  1:198441/71‑1 (MQ=255)                                                       cgcAGATGAGTCTGTAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGAAACCGGGCAGaaa              <  1:3450514/71‑1 (MQ=255)                                                         cAGATGAGTCTGTAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGAAACCGGGCAGaaaa             >  1:275361/1‑70 (MQ=255)                                                         cAGATGAGTATGTAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGAAACCGGGCAGAAAAt            >  1:485188/1‑71 (MQ=255)                                                            aTGAGTCTGCAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGAAACCGGGCAGAAAATGCt         >  1:1907032/1‑71 (MQ=255)                                                                  cTGCAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGAAAc                        >  1:240317/1‑50 (MQ=255)                                                                   tGCAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGAAACCGGGCAGAAAATGCTGGAAGCg  >  1:375716/1‑71 (MQ=255)                                                                     cAACAGCAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGAAACCGGGCAGAAAATGCTGGAAGCg  >  1:70270/1‑69 (MQ=255)                                                                     |                                                                     CTATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGAAACCGGGCAGAAAATGCTGGAAGCG  >  W3110S.gb/866988‑867124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑