| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 940,557 | A→G | 14.6% | P236P (CCA→CCG) | serS → | seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 940,557 | 0 | A | G | 14.6% | 118.5 / 13.4 | 48 | P236P (CCA→CCG) | serS | seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine |
| Reads supporting (aligned to +/- strand): ref base A (20/21); new base G (2/5); total (22/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGTTCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTGCCAATTAA > W3110S.gb/940498‑940625 | cTGTTCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGt > 1:215361/1‑71 (MQ=255) ttCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGa < 1:1145974/65‑1 (MQ=255) ttCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGa < 1:1114790/65‑1 (MQ=255) ttCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGa < 1:302852/65‑1 (MQ=255) ttCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGa < 1:604674/65‑1 (MQ=255) ttCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAg < 1:1735626/67‑1 (MQ=255) ccATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGa < 1:3644259/63‑1 (MQ=255) ccATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGc > 1:550943/1‑71 (MQ=255) cATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCt < 1:2156292/71‑1 (MQ=255) cTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGaa > 1:705876/1‑59 (MQ=255) cTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGa > 1:1705939/1‑69 (MQ=255) cGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTg < 1:69543/71‑1 (MQ=255) gaagaagCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCgg > 1:1183979/1‑71 (MQ=255) aagCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCt < 1:1547136/58‑1 (MQ=255) gCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAat > 1:3538923/1‑71 (MQ=255) gACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGtt > 1:1244965/1‑39 (MQ=255) gACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAatcat > 1:1524440/1‑71 (MQ=255) cacCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCg < 1:896460/71‑1 (MQ=255) cacCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCg < 1:931813/71‑1 (MQ=255) acCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGa > 1:2347720/1‑71 (MQ=255) cAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGAt > 1:3849752/1‑70 (MQ=255) cAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATg < 1:1689290/71‑1 (MQ=255) aGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGc > 1:4016986/1‑37 (MQ=255) aGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGc > 1:293511/1‑37 (MQ=255) aGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGc > 1:2625415/1‑37 (MQ=255) aGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGc > 1:2620765/1‑37 (MQ=255) aGTAACTATGCGCTGATCACAACGGCAGAAGTTCCGc > 1:2921013/1‑37 (MQ=255) gTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCt > 1:1831341/1‑46 (MQ=255) tAACTATGCGCTGATCCCGACTGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAat < 1:3123389/60‑1 (MQ=255) aCTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAgat > 1:2170742/1‑71 (MQ=255) aCTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAga > 1:491005/1‑70 (MQ=255) cTATGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGa < 1:2792783/54‑1 (MQ=255) cTATGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGa < 1:775885/54‑1 (MQ=255) cTATGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGa < 1:2017663/54‑1 (MQ=255) cTATGCGCTGATCCCAACGTCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAgatg < 1:2206087/71‑1 (MQ=255) cTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAgatg < 1:2722016/71‑1 (MQ=255) tGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAAcc < 1:1023324/38‑1 (MQ=255) tGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAAcc < 1:3305164/38‑1 (MQ=255) tGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAgatga < 1:601422/69‑1 (MQ=255) tGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:3880736/1‑71 (MQ=255) tGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:12865/1‑71 (MQ=255) cgcTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTg > 1:3671475/1‑38 (MQ=255) aTCCCAACGTCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAatcatc < 1:1320358/51‑1 (MQ=255) aTCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAatcatc < 1:3636536/51‑1 (MQ=255) cccAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTGCCAAtt > 1:1893965/1‑70 (MQ=255) ccAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTGCGAATTaa < 1:8204/71‑1 (MQ=255) ccAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTGCCAATTaa < 1:2284597/71‑1 (MQ=255) ccAACGCCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTGCCAATTaa < 1:628535/71‑1 (MQ=255) | CTGTTCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTGCCAATTAA > W3110S.gb/940498‑940625 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |