| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,006,848 | A→T | 12.8% | K159* (AAA→TAA) | ycbW → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,006,848 | 0 | A | T | 12.8% | 100.1 / 8.1 | 39 | K159* (AAA→TAA) | ycbW | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (7/27); new base T (3/2); total (10/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.56e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01 | |||||||||||
CGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACG > W3110S.gb/1006778‑1006918 | cGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTa < 1:339502/71‑1 (MQ=255) tATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTaaa < 1:2750821/68‑1 (MQ=255) aTGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCa > 1:1749627/1‑71 (MQ=255) gCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCAt > 1:2244883/1‑70 (MQ=255) ccTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATg < 1:1113689/70‑1 (MQ=255) ccTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATg < 1:788261/70‑1 (MQ=255) ccTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATg < 1:3547898/70‑1 (MQ=255) ccTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATg < 1:2030912/70‑1 (MQ=255) ccTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATg < 1:2587994/70‑1 (MQ=255) ctgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAAc < 1:1888766/69‑1 (MQ=255) ctgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAAc < 1:3826651/69‑1 (MQ=255) tgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGa > 1:895968/1‑70 (MQ=255) cccTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGaa < 1:913443/71‑1 (MQ=255) gttATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAgg < 1:1313021/70‑1 (MQ=255) ttATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAg < 1:1587798/55‑1 (MQ=255) ttATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGtt < 1:3888050/71‑1 (MQ=255) gCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGt > 1:3869215/1‑71 (MQ=255) gTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGc < 1:1397107/57‑1 (MQ=255) tCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGtt < 1:298937/67‑1 (MQ=255) aTGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGaa < 1:143715/44‑1 (MQ=255) gCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGa > 1:2548616/1‑60 (MQ=255) gCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGa > 1:3352408/1‑60 (MQ=255) gCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGa > 1:3038107/1‑60 (MQ=255) gTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCg < 1:3671659/71‑1 (MQ=255) gTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCg < 1:313885/71‑1 (MQ=255) tGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGtt < 1:3192269/53‑1 (MQ=255) cGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTaa < 1:3844413/42‑1 (MQ=255) cGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTaa < 1:2353593/42‑1 (MQ=255) ctaTTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCa < 1:2479106/66‑1 (MQ=255) ccATTAAAATCATGAACGACAGGCTGAAACCGCAGGtt < 1:4067342/38‑1 (MQ=255) cATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCt < 1:3950680/71‑1 (MQ=255) aTTAAAATCATGAACGACAGGCTGAAACCGCAGGTTaa < 1:2786004/38‑1 (MQ=255) aTTAAAATCATGAACGACAGGCTGAAACCGCAGGTTaa < 1:3907611/38‑1 (MQ=255) ttAAAATGATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAgg < 1:211718/63‑1 (MQ=255) ttAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATg < 1:3044226/39‑1 (MQ=255) ttAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCa < 1:891384/65‑1 (MQ=255) ttAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCt < 1:878864/69‑1 (MQ=255) ttAAAATCATGAAC‑ACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCg < 1:3650751/56‑1 (MQ=255) tAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATg > 1:843820/1‑38 (MQ=255) tAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAAc > 1:1590376/1‑71 (MQ=255) tAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAAc > 1:3041751/1‑71 (MQ=255) aaaaTCATGAACGACAGGCTGAAACCGCAGGTTaa > 1:2370683/1‑35 (MQ=255) aaaaTCATGAACGACAGGCTGAAACCGCAGGTTAAt > 1:381052/1‑36 (MQ=255) aaaaTCATGAACGACAGGCTGAAACCGCAGGTTAAt > 1:2214089/1‑36 (MQ=255) aaaaTCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGa > 1:1748237/1‑41 (MQ=255) aaaaTCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAg > 1:58450/1‑44 (MQ=255) aaaaTCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGaa > 1:1641077/1‑57 (MQ=255) aaaaTCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGaa > 1:697198/1‑57 (MQ=255) aaaaTCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGaa > 1:3336328/1‑57 (MQ=255) aaaaTCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAAcg > 1:447085/1‑71 (MQ=255) aaaaTCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAAcg > 1:2051809/1‑71 (MQ=255) aaaaTCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAAcg > 1:2147118/1‑71 (MQ=255) | CGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACG > W3110S.gb/1006778‑1006918 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |