Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,006,848 A→T 12.8% K159* (AAA→TAA)  ycbW → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,006,8480AT12.8% 100.1 / 8.1 39K159* (AAA→TAA) ycbWhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (7/27);  new base T (3/2);  total (10/29)
Fisher's exact test for biased strand distribution p-value = 9.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01

CGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACG  >  W3110S.gb/1006778‑1006918
                                                                      |                                                                      
cGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTa                                                                        <  1:339502/71‑1 (MQ=255)
     tATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTaaa                                                                      <  1:2750821/68‑1 (MQ=255)
      aTGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCa                                                                  >  1:1749627/1‑71 (MQ=255)
        gCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCAt                                                                 >  1:2244883/1‑70 (MQ=255)
         ccTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATg                                                                <  1:1113689/70‑1 (MQ=255)
         ccTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATg                                                                <  1:788261/70‑1 (MQ=255)
         ccTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATg                                                                <  1:3547898/70‑1 (MQ=255)
         ccTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATg                                                                <  1:2030912/70‑1 (MQ=255)
         ccTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATg                                                                <  1:2587994/70‑1 (MQ=255)
             ctgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAAc                                                             <  1:1888766/69‑1 (MQ=255)
             ctgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAAc                                                             <  1:3826651/69‑1 (MQ=255)
              tgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGa                                                           >  1:895968/1‑70 (MQ=255)
                      cccTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGaa                                                  <  1:913443/71‑1 (MQ=255)
                               gttATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAgg                                          <  1:1313021/70‑1 (MQ=255)
                                ttATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAg                                                        <  1:1587798/55‑1 (MQ=255)
                                ttATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGtt                                        <  1:3888050/71‑1 (MQ=255)
                                     gCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGt                                   >  1:3869215/1‑71 (MQ=255)
                                         gTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGc                                             <  1:1397107/57‑1 (MQ=255)
                                          tCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGtt                                  <  1:298937/67‑1 (MQ=255)
                                                 aTGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGaa                                                  <  1:143715/44‑1 (MQ=255)
                                                   gCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGa                                >  1:2548616/1‑60 (MQ=255)
                                                   gCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGa                                >  1:3352408/1‑60 (MQ=255)
                                                   gCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGa                                >  1:3038107/1‑60 (MQ=255)
                                                      gTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCg                  <  1:3671659/71‑1 (MQ=255)
                                                      gTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCg                  <  1:313885/71‑1 (MQ=255)
                                                        tGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGtt                                  <  1:3192269/53‑1 (MQ=255)
                                                               cGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTaa                                      <  1:3844413/42‑1 (MQ=255)
                                                               cGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTaa                                      <  1:2353593/42‑1 (MQ=255)
                                                                 ctaTTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCa          <  1:2479106/66‑1 (MQ=255)
                                                                 ccATTAAAATCATGAACGACAGGCTGAAACCGCAGGtt                                        <  1:4067342/38‑1 (MQ=255)
                                                                  cATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCt      <  1:3950680/71‑1 (MQ=255)
                                                                   aTTAAAATCATGAACGACAGGCTGAAACCGCAGGTTaa                                      <  1:2786004/38‑1 (MQ=255)
                                                                   aTTAAAATCATGAACGACAGGCTGAAACCGCAGGTTaa                                      <  1:3907611/38‑1 (MQ=255)
                                                                    ttAAAATGATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAgg            <  1:211718/63‑1 (MQ=255)
                                                                    ttAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATg                                    <  1:3044226/39‑1 (MQ=255)
                                                                    ttAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCa          <  1:891384/65‑1 (MQ=255)
                                                                    ttAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCt      <  1:878864/69‑1 (MQ=255)
                                                                    ttAAAATCATGAACACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCg                  <  1:3650751/56‑1 (MQ=255)
                                                                     tAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATg                                    >  1:843820/1‑38 (MQ=255)
                                                                     tAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAAc   >  1:1590376/1‑71 (MQ=255)
                                                                     tAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAAc   >  1:3041751/1‑71 (MQ=255)
                                                                      aaaaTCATGAACGACAGGCTGAAACCGCAGGTTaa                                      >  1:2370683/1‑35 (MQ=255)
                                                                      aaaaTCATGAACGACAGGCTGAAACCGCAGGTTAAt                                     >  1:381052/1‑36 (MQ=255)
                                                                      aaaaTCATGAACGACAGGCTGAAACCGCAGGTTAAt                                     >  1:2214089/1‑36 (MQ=255)
                                                                      aaaaTCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGa                                >  1:1748237/1‑41 (MQ=255)
                                                                      aaaaTCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAg                             >  1:58450/1‑44 (MQ=255)
                                                                      aaaaTCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGaa                >  1:1641077/1‑57 (MQ=255)
                                                                      aaaaTCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGaa                >  1:697198/1‑57 (MQ=255)
                                                                      aaaaTCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGaa                >  1:3336328/1‑57 (MQ=255)
                                                                      aaaaTCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAAcg  >  1:447085/1‑71 (MQ=255)
                                                                      aaaaTCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAAcg  >  1:2051809/1‑71 (MQ=255)
                                                                      aaaaTCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAAcg  >  1:2147118/1‑71 (MQ=255)
                                                                      |                                                                      
CGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACG  >  W3110S.gb/1006778‑1006918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: