| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,023,649 | G→A | 24.2% | L219L (CTC→CTT) | yccS ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,023,649 | 0 | G | A | 24.2% | 60.2 / 16.9 | 33 | L219L (CTC→CTT) | yccS | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (12/13); new base A (6/2); total (18/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTAATAATGCAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCAAATCGTACAGCGG > W3110S.gb/1023582‑1023711 | aGTAATAATGCAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGt < 1:810962/71‑1 (MQ=255)aGTAATAATGCAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGt < 1:2067631/71‑1 (MQ=255) atGCAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCt < 1:1245186/71‑1 (MQ=255) atGCAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCt > 1:1388723/1‑71 (MQ=255) atGCAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCt > 1:1579399/1‑71 (MQ=255) atGCAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCt < 1:4823/71‑1 (MQ=255) cAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGAt > 1:452822/1‑71 (MQ=255) gCGTGCGACGCGTTCCCCGTTGACCACGTTCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTc < 1:3940882/71‑1 (MQ=255) gCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTc < 1:394457/71‑1 (MQ=255) gCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAAAGTTTCGTCTGATTc < 1:1872031/71‑1 (MQ=255) gCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAAAGTTTCGTCTGATTc < 1:1867136/71‑1 (MQ=255) cGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCa < 1:2841454/71‑1 (MQ=255) gCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATg > 1:3501139/1‑71 (MQ=255) cGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGt > 1:1879717/1‑71 (MQ=255) gcgTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTc > 1:3422725/1‑68 (MQ=255) gcgTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAAAGTTTCGTCTGATTCAATGTCGc > 1:3985587/1‑70 (MQ=255) ttGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGAc < 1:3641252/71‑1 (MQ=255) ttGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGAc < 1:2657694/71‑1 (MQ=255) ttGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGAc < 1:1717409/71‑1 (MQ=255) gACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTc > 1:458848/1‑44 (MQ=255) aCGATCGCCACGTAAGCGGGTCAGCAGCGAAAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTgg > 1:2059402/1‑71 (MQ=255) cGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGa > 1:3411584/1‑42 (MQ=255) cGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGa > 1:3511187/1‑42 (MQ=255) cGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGa > 1:2027518/1‑42 (MQ=255) gCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCa < 1:2870306/41‑1 (MQ=255) aCGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCa < 1:862143/71‑1 (MQ=255) aCGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCa < 1:1218872/71‑1 (MQ=255) aCGTAAGCGGGTCAGCAGCGAAAGTTTCGTCTGATTCaa > 1:3601310/1‑39 (MQ=255) aCGTAAGCGGGTCAGCAGCGAAAGTTTCGTCTGATTCaa > 1:3182161/1‑39 (MQ=255) aCGTAAGCGGGTCAGCAGCGAAAGTTTCGTCTGATTCaa > 1:2614350/1‑39 (MQ=255) aCGTAAGCGGGTCAGCAGCGAAAGTTTCGTCTGATTCaa > 1:2596275/1‑39 (MQ=255) tAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGcc > 1:2459845/1‑43 (MQ=255) agcagcGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCAAATCGTACAGCgg > 1:104961/1‑71 (MQ=255) | AGTAATAATGCAGCGTGCGACGCGTTCCCCGTTGACCACGATCGCCACGTAAGCGGGTCAGCAGCGAGAGTTTCGTCTGATTCAATGTCGCCATCAGCAGACCGTTGGCGAGAGCCAAATCGTACAGCGG > W3110S.gb/1023582‑1023711 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |