| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,029,877 | C→G | 22.2% | G143A (GGC→GCC) | yccW ← | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,029,877 | 0 | C | G | 22.2% | 47.4 / 10.3 | 27 | G143A (GGC→GCC) | yccW | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (15/6); new base G (2/4); total (17/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGACGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAAC > W3110S.gb/1029816‑1029940 | cagTTTCATTCCGTGTTCTT‑AATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGACGGGGCCCTGGGTCAg > 1:3886628/1‑71 (MQ=255) tttCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGACGGGGCCCTGGGTCa < 1:1075648/68‑1 (MQ=255) tCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGACGGGGGCCTGGGTCa > 1:1281987/1‑66 (MQ=255) tCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGACGGGGCCCTGGGTCAGCt > 1:3412888/1‑69 (MQ=255) ccGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGACGGGGCCCTGGGTCAGCTCCATTcc > 1:1751662/1‑71 (MQ=255) ccGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGACGGGGCCCTGGGTCAGCTCCATTcc > 1:88529/1‑71 (MQ=255) ccGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGACGGGGCCCTGGGTCAGCTCCATTc > 1:1578466/1‑70 (MQ=255) ttcttcAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGACGGGGCCCTGGGTCAGCTCCATTCcttctt > 1:2071679/1‑71 (MQ=255) ttcttcAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGACGGGGCCCTGGGTCAGCTCCATTCcttctt > 1:3735362/1‑71 (MQ=255) ttcttcAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGACGGGGCCCTGGGTCAGCTCCATTCcttct < 1:2211650/70‑1 (MQ=255) cttcAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGACGGGGCCCTGGGTCAGCTCCATTCCTTCtttt > 1:970206/1‑71 (MQ=255) cttcAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGACGGGGCCCTGGGTCAGCTCCATTCCTTCtttt > 1:1868187/1‑71 (MQ=255) aaTCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGACGGGGCCCT‑‑GTCAGCTCCATTCCTTCTTTTTTACGt < 1:123355/71‑1 (MQ=255) tCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGACGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGt < 1:754506/71‑1 (MQ=255) caggTGGCAACTCGCCGGTGACGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCg > 1:366892/1‑71 (MQ=255) caggTGGCAACTCGCCGGTGACGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCg > 1:371580/1‑71 (MQ=255) gTGGCAACTCGCCGGTGACGGGGGCCTGGGTCAGCTCCATTCCTTCttt < 1:3398011/49‑1 (MQ=255) gTGGCAACTCGCCGGTGACGGGGGCCTGGGTCAGCTCCATTCCTTCttt < 1:819016/49‑1 (MQ=255) ggCAACTCGCCGGTGACGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGc > 1:3008612/1‑70 (MQ=255) gCAACTCGCCGGTGACGGGGCCCTGGGTCAGCTCCATTCct > 1:1646283/1‑41 (MQ=255) aaCTCGCCGGTGACGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATc > 1:3968296/1‑71 (MQ=255) tCGCCGGTGACGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTa > 1:3397089/1‑45 (MQ=255) cGCCGGTGACGGGGGCCTGGGTCAGCTCCATTCCTTCttt > 1:212641/1‑40 (MQ=255) cGGTGACGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATc < 1:2326198/71‑1 (MQ=255) tGACGGGGGCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGaa < 1:3338884/71‑1 (MQ=255) gACGGGGGCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGaa < 1:908578/70‑1 (MQ=255) aCGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAAc < 1:3295184/70‑1 (MQ=255) | CAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGACGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAAC > W3110S.gb/1029816‑1029940 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |