| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,044,904 | T→C | 38.7% | E65E (GAA→GAG) | etp ← | phosphotyrosine‑protein phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,044,904 | 0 | T | C | 38.7% | 53.6 / 85.0 | 75 | E65E (GAA→GAG) | etp | phosphotyrosine‑protein phosphatase |
| Reads supporting (aligned to +/- strand): ref base T (27/19); new base C (7/22); total (34/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.36e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01 | |||||||||||
CATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTACCAGAC > W3110S.gb/1044841‑1044971 | cATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAaga < 1:2531333/70‑1 (MQ=255)cATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCCTCaa > 1:2957315/1‑68 (MQ=255)cATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCCTCaa > 1:3656910/1‑68 (MQ=255) aTTGGCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAgaga < 1:1605462/71‑1 (MQ=255) aTTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAgaga < 1:501920/71‑1 (MQ=255) gCCATAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATAc > 1:3191395/1‑71 (MQ=255) gCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATAc > 1:3518402/1‑71 (MQ=255) aaTCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCAt < 1:3610116/71‑1 (MQ=255) aaTCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCCTCAAGAGATACGCCAt < 1:1047265/71‑1 (MQ=255) aTCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCa < 1:188785/57‑1 (MQ=255) aTCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCCTCAAGAGATACGCCATg < 1:2571319/71‑1 (MQ=255) tCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATAc < 1:430585/64‑1 (MQ=255) tCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATAc < 1:2825882/64‑1 (MQ=255) tCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGa > 1:3974140/1‑71 (MQ=255) tCAGATCGTAATTTCGTGCCATATCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGa > 1:3707044/1‑71 (MQ=255) aTCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCCTCAaga < 1:3887621/55‑1 (MQ=255) aTCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCCTCAaga < 1:3418905/55‑1 (MQ=255) aTCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCCTCAaga < 1:3599979/55‑1 (MQ=255) aTCGAAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCCTCAaga < 1:3944976/55‑1 (MQ=39) tCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACTCCa > 1:3607693/1‑63 (MQ=255) cGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTa > 1:2686835/1‑68 (MQ=255) cGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTa > 1:314671/1‑68 (MQ=255) cGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTa > 1:1805996/1‑68 (MQ=255) aTTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAaga > 1:798748/1‑49 (MQ=255) tCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATa > 1:3902506/1‑50 (MQ=255) tCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATa > 1:727266/1‑50 (MQ=255) tCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATa > 1:3897092/1‑50 (MQ=255) tCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACa > 1:3226361/1‑70 (MQ=255) tCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACAt > 1:2061257/1‑71 (MQ=255) tCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACAt > 1:741335/1‑71 (MQ=255) cGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCCTCAAGAGATACGCCATGATTAGCGGCAACAt < 1:178597/70‑1 (MQ=255) aTCTCAGCAGTGAGCTTACGTCCGGCATGTCCCTCAAGAGATACGCCATGATTAGCGGCAAc < 1:1543089/62‑1 (MQ=255) aTCTCAGCAGTGAGCTTACGTCCGGCATGTCCCTCAAGAGATACGCCATGATTAGCGGCAAc < 1:379815/62‑1 (MQ=255) tctcAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGc < 1:1719948/45‑1 (MQ=255) tctcAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGc < 1:3766479/45‑1 (MQ=255) tctcAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCt < 1:1042359/65‑1 (MQ=255) tctcAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCt < 1:1143785/65‑1 (MQ=255) tctcAGCAGTGAGCTTACGTCCGGCATGTCCCTCAAGAGATACGCCATGAtt < 1:891604/52‑1 (MQ=255) tctcAGCAGTGAGCTTACGTCCGGCATGTCCCTCAAGAGATACGCCATGAtt < 1:1408417/52‑1 (MQ=255) tctcAGCAGTGAGCTTACGTCCGGCATGTCCCTCAAGAGATACGCCATGAtt < 1:2077440/52‑1 (MQ=255) tctcAGCAGTGAGCTTACGTCCGGCATGTCCCTCAAGAGATACGCCATGAtt < 1:162150/52‑1 (MQ=255) tctcAGCAGTGAGCTTACGTCCGGCATGTCCCTCAAGAGATACGCCATGATTAGCGGCAAc < 1:3304665/61‑1 (MQ=255) tctcAGCAGTGAGCTTACGTCCGGCATGTCCCTCAAGAGATACGCCATGATTAGCGGCAAc < 1:826524/61‑1 (MQ=255) tcAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGc > 1:1016754/1‑65 (MQ=255) tcAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGc > 1:273944/1‑65 (MQ=255) tcAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGt > 1:3949883/1‑71 (MQ=255) cagTGAGCTTACGTCCGGCATGTCCCTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCa < 1:3489807/71‑1 (MQ=255) tGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACa < 1:4021385/53‑1 (MQ=255) tGAGCTTACGTCCGGCATGTCCCTCAAGAGATACGCCATGATTAGCGGc < 1:4002799/49‑1 (MQ=255) tGAGCTTACGTCCGGCATGTCCCTCAAGAGATACGCCATGATTAGCGGc < 1:2512493/49‑1 (MQ=255) tGAGCTTACGTCCGGCATGTCCCTCAAGAGATACGCCATGATTAGCGGCAACa < 1:35801/53‑1 (MQ=255) tGAGCTTACGTCCGGCATGTCCCTCAAGAGATACGCCATGATTAGCGGCAACa < 1:866152/53‑1 (MQ=255) gAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGa > 1:933061/1‑40 (MQ=255) aGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTg < 1:3419626/70‑1 (MQ=255) aGCTTAAGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTg > 1:4161/1‑70 (MQ=255) gCTTACGTCCGGCATGTCCCTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTg > 1:2445883/1‑69 (MQ=255) gCTTACGTCCGGCATGTCCCTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTg > 1:909420/1‑69 (MQ=255) gCTTACGTCCGGCATGTCCCTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCa < 1:1180059/71‑1 (MQ=255) gCTTACGTCCGGCATGTCCCTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCa < 1:1142206/71‑1 (MQ=255) tACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTa > 1:2943701/1‑38 (MQ=255) tACGTCCGGCATGTCCCTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAggg > 1:349281/1‑71 (MQ=255) tACGTCCGGCATGTCCCTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAggg > 1:531426/1‑71 (MQ=255) aCGTCCGGCATGTCCTTCAAGAGATACGCCATGATTA‑‑GGCAACATCTGc > 1:982028/1‑49 (MQ=38) cGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGgtg < 1:1364099/71‑1 (MQ=255) cGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGttt > 1:2431066/1‑70 (MQ=255) cGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGttt > 1:2204149/1‑70 (MQ=255) gCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTAc < 1:334852/71‑1 (MQ=255) gCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTAc < 1:3273600/71‑1 (MQ=255) gCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTAc < 1:1880531/71‑1 (MQ=255) aTGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTACCa < 1:3412099/71‑1 (MQ=255) aTGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTACCa < 1:1280107/71‑1 (MQ=255) tCCTTCAAGAGATACGCCATGATTAGCGGCAACAt > 1:818709/1‑35 (MQ=255) tCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGttt > 1:437830/1‑63 (MQ=255) tCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGttt > 1:3399888/1‑63 (MQ=255) tCCCTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTACCAGAc > 1:2983706/1‑71 (MQ=255) | CATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTACCAGAC > W3110S.gb/1044841‑1044971 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |