| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,082,168 | G→A | 15.6% | pseudogene (391/830 nt) pseudogene (293/732 nt) |
ycdN → ycdN → |
ECK1007:JW5141+JW1002:b4490; predicted membrane protein ECK1007:JW1002:b1017; hypothetical protein, C‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,082,168 | 0 | G | A | 15.6% | 78.4 / 7.4 | 32 | pseudogene (391/830 nt) pseudogene (293/732 nt) | ycdN ycdN | ECK1007:JW5141+JW1002:b4490; predicted membrane protein ECK1007:JW1002:b1017; hypothetical protein, C‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base G (12/15); new base A (3/2); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
TTGCAGCGTGGAAATCATCATGGCTGGGCGCTGGTGATGATGGTCTTTTTTGCCGTTGCAAGGGAAGGGCTGGAGTCGGTCTTTTTCCTGCTGGCGGCATTTCAACAAGATGTCGGGATCTGGCCGCCGCTGGGTGC > W3110S.gb/1082101‑1082237 | ttGCAGCGTGGAAATCATCATGGCTGGGCGCTGGTGATGATGGTCTTTTTTGCCGTTGCAAGGGAAGGGCt < 1:2687545/71‑1 (MQ=255) tGCAGCGTGGAAATCATCATGGCTGGGCGCTGGTGATGATGGTCTTTTTTGCCGTTGCAAGGGAAGGGCTg > 1:1661050/1‑71 (MQ=255) tGCAGCGTGGAAATCATCATGGCTGGGCGCTGGTGATGATGGTCTTTTTTGCCGTTGCAAGGGAAGGGCTg > 1:3819894/1‑71 (MQ=255) gCAGCGTGGAAATCATCATGGCTGGGCGCTGGTGATGATGGTCTTTTTTGCCGTTGCAAGGGAAGGGCTg < 1:382906/70‑1 (MQ=255) gTGGAAATCATCATGGCTGGGCGCTGGTGATGATGGTCTTTTTTGCCGTTGCAAGGGAAGGGCTGGAGTCg > 1:740917/1‑71 (MQ=255) tGGAAATCATCATGGCTGGGCGCTGGTGATGATGGTCTTTTTTGCCGTTGCAAGGGAAGGGCTGGAGTCgg < 1:1169198/71‑1 (MQ=255) gAAATCATCATGGCTGGGCGCTGGTGATGATGGTCTTTTTTGCCGTTGCAAGGGAAGGGCTGGAGTCGGt > 1:1567106/1‑70 (MQ=255) ggCTGGGCGCTGGTGATGATGGTCTTTTTTGCCGTTGCAAGGGAAGGGCTGGAGTCGGTCTTTTTCctgct > 1:2953260/1‑71 (MQ=255) gcgcTGGTGATGATGGTCTTTTTTGCCGTTGCAAGGGAAGGGCTGGAGTCGGTCTTTTTCCTGCTggcgg > 1:697012/1‑70 (MQ=255) gTGATGATGGTCTTTTTTGCCGTTGCAAGGGAAGGGc < 1:840118/37‑1 (MQ=255) gTGATGATGGTCTTTTTTGCCGTTGCAAGGGAAGGGc < 1:3302603/37‑1 (MQ=255) gTGATGATGGTCTTTTTTGCCGTTGCAAGGGAAGGGCTGGAGTCGGTCTTTTTCCTGCTGGCGGCATTTc > 1:3718211/1‑70 (MQ=255) tgatgaTGGTCTTTTTTGCCGTTGCAAGGGAAGAGCTg < 1:2278755/38‑1 (MQ=255) atgatgGTCTTTTTTGCCGTTGCAAGGGAAGGGCTg < 1:3330496/36‑1 (MQ=255) atgatgGTCTTTTTTGCCGTTGCAAGGGAAGAGCTGGAGTCGGTCTTTTTCCTGCTGGCGGCATTTcaac < 1:3881652/70‑1 (MQ=255) atgGTCTTTTTTGCCGTTGCAAGGGAAGGGCTGGAGTCGGTCTTTTTCCTGCTGGCGGCATTTcaac > 1:4021327/1‑67 (MQ=255) atgGTCTTTTTTGCCGTTGCAAGGGAAGGGCTGGAGTCGGTCTTTTTCCTGCTGGCGGCATTTCAACAAg < 1:3458239/70‑1 (MQ=255) atgGTCTTTTTTGCCGTTGCAAGGGAAGAGCTGGAGTCGGTCTTTTTCCTGCTGGCGGCATTTcaac > 1:2886240/1‑67 (MQ=255) tgGTCTTTTTTGCCGTTGCAAGGGAAGGGCTGGAGTCGGTCtttt < 1:3687625/45‑1 (MQ=255) ggTCTTTTTTGCCGTTGCAAGGGAAGGGCTGGAGTCGGTCttttt < 1:2707079/45‑1 (MQ=255) tCTTTTTTGCCGTTGCAAGGGAAGGGCTGGAGTCGGTCTTTTTCCTGCTggcgg < 1:2447957/54‑1 (MQ=255) tCTTTTTTGCCGTTGCAAGGGAAGGGCTGGAGTCGGTCTTTTTCCTGCTggcgg < 1:2095109/54‑1 (MQ=255) cTTTTTTGCCGTTGCAAGGGAAGAGCTGGAGTCGGTCttt > 1:3442502/1‑40 (MQ=255) cTTTTTAGCCGTTGCAAGGGAAGAGCTGGAGTCGGTCtttt > 1:525178/1‑41 (MQ=38) ttttGCCGTTGCAAGGGAAGGGCTGGAGTCGGTCTTTTTCCTGCTGGCGGCATTTCAACAAGATGTCggg < 1:3689090/70‑1 (MQ=255) ttttGCCGTTGCAAGGGAAGGGCTGGAGTCGGTCTTTTTCCTGCTGGCGGCATTTCAACAAGATGTCggg < 1:3150819/70‑1 (MQ=255) ttttGCCGTTGCAAGGGAAGGGCTGGAGTCGGTCTTTTTCCTGCTGGCGGCATTTCAACAAGATGTCggg < 1:1724468/70‑1 (MQ=255) ttGCAAGGGAAGGGCTGGAGTCGGTCTTTTTCCTGCTGGCGGCATTTcaaca < 1:3658086/52‑1 (MQ=255) gCAAGGGAAGGGCTGGAGTCGGTCTTTTTCCTGCTGGCGGCATTTCAACAAGATGt > 1:3226523/1‑56 (MQ=255) gCAAGGGAAGGGCTGGAGTCGGTCTTTTTCCTGCTGGCGGCATTTCAACAAGATGt > 1:2246598/1‑56 (MQ=255) gCAAGGGAAGGGCTGGAGTCGGTCTTTTTCCTGCTGGCGGCATTTCAACAAGATGTCGGGATCTGgccgcc > 1:3026655/1‑71 (MQ=255) gCAAGGGAAGGGCTGGAGTCGGTCTTTTTCCTGCTGGCGGCATTTCAACAAGATGTCGGGATCTGgccgc > 1:1832145/1‑70 (MQ=255) gaagggCTGGAGTCGGTCTTTTTCCTGCTGGCGGAATTTCAACAAGATGTCGGGATCTGGCCGCCGCTggg > 1:1400836/1‑71 (MQ=255) gggCTGGAGTCGGTCTTTTTCCTGCTGGCGGCATTTCAACAAGATGTCGGGATCTGGCCGCCGCTGGGTGc < 1:3528040/71‑1 (MQ=255) gggCTGGAGTCGGTCTTTTTCCTGCTGGCGGCATTTCAACAAGATGTCGGGATCTGGCCGCCGCTGGGTGc < 1:3297463/71‑1 (MQ=255) gggCTGGAGTCGGTCTTTTTCCTGCTGGCGGCATTTCAACAAGATGTCGGGATCTGGCCGCCGCTGGGTGc < 1:2787901/71‑1 (MQ=255) gggCTGGAGTCGGTCTTTTTCCTGCTGGCGGCATTTCAACAAGATGTCGGGATCTGGCCGCCGCTGGGTGc < 1:2370607/71‑1 (MQ=255) gggCTGGAGTCGGTCTTTTTCCTGCTGGCGGCATTTCAACAAGATGTCGGGATCTGGCCGCCGCTGGGTGc < 1:2156851/71‑1 (MQ=255) gggCTGGAGTCGGTCTTTTTCCTGCTGGCGGCATTTCAACAAGATGTCGGGATCTGGCCGCCGCTGGGTGc < 1:1975606/71‑1 (MQ=255) | TTGCAGCGTGGAAATCATCATGGCTGGGCGCTGGTGATGATGGTCTTTTTTGCCGTTGCAAGGGAAGGGCTGGAGTCGGTCTTTTTCCTGCTGGCGGCATTTCAACAAGATGTCGGGATCTGGCCGCCGCTGGGTGC > W3110S.gb/1082101‑1082237 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |