| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,207,066 | A→G | 20.6% | E440E (GAA→GAG) | ymfN → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,207,066 | 0 | A | G | 20.6% | 67.2 / 14.4 | 34 | E440E (GAA→GAG) | ymfN | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (15/12); new base G (3/4); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
CGGAAAACAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTATGATCATGCT > W3110S.gb/1206996‑1207136 | cGGAAAACAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGagaaagaa < 1:1296738/71‑1 (MQ=255)cGGAAAACAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGagaaagaa < 1:3453319/71‑1 (MQ=255)cGGAAAACAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGagaaagaa < 1:1593971/71‑1 (MQ=255) aaaaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGagaaagaa < 1:1032600/68‑1 (MQ=255) aaaaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGagaaagaa < 1:1172347/68‑1 (MQ=255) aaaaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGagaaagaa < 1:1291908/68‑1 (MQ=255) aaaaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGagaaagaa < 1:3627874/68‑1 (MQ=255) aaaaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGagaaagaa < 1:2403904/68‑1 (MQ=255) aaaaTCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCGTGCTGTACGAGAAAGAAGACACGCt < 1:782536/71‑1 (MQ=255) gATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGa > 1:168316/1‑71 (MQ=255) ggTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTATGAGAAAGAGGACACGCTGTCTGATca > 1:452218/1‑71 (MQ=255) ggTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATca > 1:45726/1‑71 (MQ=255) gTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATcac < 1:2473335/71‑1 (MQ=255) gTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATca < 1:470031/70‑1 (MQ=255) tGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATca < 1:2615614/69‑1 (MQ=255) cAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACAtt < 1:1980648/71‑1 (MQ=255) cAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACAtt < 1:453294/71‑1 (MQ=255) ttGCGCTGATTATGGCGGTTGGCAGAGTCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAg < 1:1365078/71‑1 (MQ=255) ttGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAg < 1:2723560/71‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAg > 1:3934709/1‑70 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGt > 1:3124349/1‑71 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGt > 1:3816543/1‑71 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGt > 1:1400452/1‑71 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGt > 1:921271/1‑71 (MQ=255) gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAagaaga > 1:419201/1‑47 (MQ=255) gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTc > 1:16451/1‑71 (MQ=255) gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTc > 1:1537249/1‑71 (MQ=255) cTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTg < 1:3938200/55‑1 (MQ=255) gATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACg > 1:3845953/1‑71 (MQ=255) tATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGac > 1:2503139/1‑40 (MQ=255) tATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGac > 1:915979/1‑40 (MQ=255) tATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGac > 1:3901359/1‑40 (MQ=255) cGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCcgc < 1:534503/71‑1 (MQ=255) ttGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACggg < 1:738304/62‑1 (MQ=255) ttGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACggg < 1:2455167/62‑1 (MQ=255) tGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCcgctcgc > 1:3472316/1‑71 (MQ=255) cAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCttt < 1:1038574/71‑1 (MQ=255) cAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCttt < 1:2480356/71‑1 (MQ=255) cAGAGCCATGATGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCttt < 1:1904194/71‑1 (MQ=255) ccATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTg < 1:3416879/71‑1 (MQ=255) cTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCcgctc > 1:261896/1‑56 (MQ=255) cTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAgg > 1:739341/1‑69 (MQ=255) cTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGt > 1:3262065/1‑70 (MQ=255) gaaagagGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAAtt < 1:3816095/66‑1 (MQ=255) gaaagagGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAAtt < 1:919758/66‑1 (MQ=255) agaggaCACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTATGATCAt > 1:3254284/1‑71 (MQ=255) agaCACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTATGATCATGCt < 1:2688312/71‑1 (MQ=255) agaCACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTATGATCATGCt < 1:2537400/71‑1 (MQ=255) agaCACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTATGATCATGCt < 1:895347/71‑1 (MQ=255) | CGGAAAACAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTATGATCATGCT > W3110S.gb/1206996‑1207136 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |