| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,237,778 | C→T | 9.7% | R347R (CGG→CGA) | ycgB ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,237,778 | 0 | C | T | 9.7% | 120.6 / 3.2 | 41 | R347R (CGG→CGA) | ycgB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (25/12); new base T (2/2); total (27/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
TCCAGCCAGTCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTA > W3110S.gb/1237710‑1237847 | tCCAGCCAGTCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCg > 1:966418/1‑71 (MQ=255)tCCAGCCAGTCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCg > 1:1541490/1‑71 (MQ=255) agTCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAata < 1:1355180/71‑1 (MQ=255) cGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATcc > 1:1176402/1‑71 (MQ=255) ggCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACa < 1:1148268/71‑1 (MQ=255) atCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCg > 1:265470/1‑70 (MQ=255) atCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCg > 1:1826227/1‑70 (MQ=255) atCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCg > 1:1645759/1‑70 (MQ=255) atCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCG‑TTAATATCCTGGAACATGGCGaa > 1:1939991/1‑71 (MQ=255) tCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGaa > 1:1893987/1‑71 (MQ=255) ccAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAggg < 1:1396712/71‑1 (MQ=255) aGTATTTGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCa < 1:546776/71‑1 (MQ=255) gTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACa < 1:3126204/53‑1 (MQ=255) tATTTGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTCGAACa < 1:3967746/52‑1 (MQ=39) tATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAAccc < 1:2875742/62‑1 (MQ=255) tttGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTaa > 1:3536565/1‑38 (MQ=255) tttGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATc > 1:644720/1‑42 (MQ=255) tttGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATc > 1:378089/1‑42 (MQ=255) tttATCTTCTTCCGTTGGCGACTGACAAATCCGTTTAat > 1:1141700/1‑39 (MQ=255) tGTCTTGTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACa > 1:521323/1‑48 (MQ=255) tGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAatat > 1:3991962/1‑39 (MQ=255) tGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAggg > 1:2175042/1‑63 (MQ=255) tGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAggg > 1:4045445/1‑63 (MQ=255) tGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACggg > 1:935678/1‑71 (MQ=255) tGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACggg > 1:653852/1‑71 (MQ=255) tcttctTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGCACATGGCGAACCCGAGGGCATACGGGtt < 1:762810/71‑1 (MQ=255) tcttctTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGtt < 1:2353581/71‑1 (MQ=255) ttcttcCGTTGGCGACTGACAAATCCGTTTAATATCCTGGaa < 1:2097283/42‑1 (MQ=255) ttcttcCGTTGGCGACTGACAAATCCGTTTAATATCCTGGaa < 1:3851663/42‑1 (MQ=255) ttcttcCGTTGCCGACTGACAAATCCGTTTAATATCCTGGaa < 1:2204650/42‑1 (MQ=255) cttcCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACa > 1:997044/1‑42 (MQ=255) cttcCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGAt < 1:3015145/70‑1 (MQ=255) cttcCGTTGGCGACTGACAAATCCGTTTAATATACTGGAACa > 1:1595328/1‑42 (MQ=255) ccGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCg > 1:3418094/1‑71 (MQ=255) ggCGACTGACAAATCCGTTTAATATCCTGGAACATgg > 1:2994499/1‑37 (MQ=255) ggCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTa > 1:1845015/1‑71 (MQ=255) gACAAATCCGTATAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACgg > 1:1782228/1‑70 (MQ=255) aCAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGc > 1:100185/1‑71 (MQ=255) aCAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGc > 1:2983678/1‑71 (MQ=255) aaaTCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTg > 1:3720198/1‑71 (MQ=255) tCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGATGTACCACGGGCTGTta < 1:1619092/71‑1 (MQ=255) | TCCAGCCAGTCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTA > W3110S.gb/1237710‑1237847 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |