| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,249,123 | G→A | 12.8% | intergenic (‑170/+150) | treA ← / ← dhaH | periplasmic trehalase/fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,249,123 | 0 | G | A | 12.8% | 103.8 / 6.9 | 39 | intergenic (‑170/+150) | treA/dhaH | periplasmic trehalase/fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component |
| Reads supporting (aligned to +/- strand): ref base G (17/17); new base A (2/3); total (19/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
CTGTCAGGCATTTTAGAAAAGGGAATTAAAGGGATAAATAACGGCGAAATAGAGTGATCAAATAACGTCTGTTTATTAGCCAGCCAGCGGCGTTGGGTTCGTGATTAGCGAAGAAAAATCATCATAAATTGGTGAAA > W3110S.gb/1249057‑1249193 | cTGTCAGGCATTTTAGAAAAGGGAATTAAAGGGATAAATAACGGCGAAATAGAGTGATCAAATAACGTCTg > 1:17504/1‑71 (MQ=255) tCAGGCATTTTAGAAAAGGGAATTAAAGGGATAAATAACGGCGAAATAGAGTGATCAAATAACGTCTGtt < 1:3911698/70‑1 (MQ=255) tCAGGCATTTTAGAAAAGGGAATTAAAGGGATAAATAACGGCGAAATAGAGTGATCAAATAACGTCTGtt < 1:2861429/70‑1 (MQ=255) tCAGGCATTTTAGAAAAGGGAATTAAAGGGATAAATAACGGCGAAATAGAGTGATCAAATAACGTCTGtt < 1:1801994/70‑1 (MQ=255) tCAGGCATTTTAGAAAAGGGAATTAAAGGGATAAATAACGGCGAAATAGAGTGATCAAATAACGTCTGtt < 1:3347477/70‑1 (MQ=255) tCAGGCATTTTAGAAAAGGGAATTAAAGGGATAAATAACGGCGAAATAGAGTGATCAAATAACGTCTGtt < 1:3860987/70‑1 (MQ=255) aGGCATTTTAGAAAAGGGAATTAAAGGGATAAATAACGGCGAAATAGAGTGATCAAATAACGTCTGTttat < 1:3954480/71‑1 (MQ=255) aGGCATTTTAGAAAAGGGAATTAAAGGGATAAATAACGGCGAAATAGAGTGATCAAATAACGTCTGTttat < 1:727645/71‑1 (MQ=255) aTTTTAGAAAAGGGAATTAAAGGGATAAATAACGGCGAAATAGAGTGATCAAATAACGTCTGTttat > 1:3028843/1‑67 (MQ=255) aTTTTAGAAAAGGGAATTAAAGGGATAAATAACGGCGAAATAGAGTGATCAAATAACGTCTGTTTATTagc > 1:1869194/1‑71 (MQ=255) ttttAGAAAAGGGAATTAAAGGGATAAATAACGGCGAAATAGAGTGATCAAATAACGTCTGtt < 1:1937889/63‑1 (MQ=255) ttttAGAAAAGGGAATTAAAGGGATAAATAACGGCGAAATAGAGTGATCAAATAACGTCTGtt < 1:761212/63‑1 (MQ=255) ttAGAAAAGGGAATTAAAGGGATAAATAACGGCGAAATAGAGTGATCAAATAACGTCTGTTTATTagccag > 1:3873723/1‑71 (MQ=255) ttAGAAAAGGGAATTAAAGGGATAAATAACGGCGAAATAGAGTGATCAAATAACGTCTGTTTATTagccag > 1:2205903/1‑71 (MQ=255) ttAGAAAAGGGAATTAAAGGGATAAATAACGGCGAAATAGAGTGATCAAATAACGTCTGTTTATTagccag > 1:3272731/1‑71 (MQ=255) ttAGAAAAGGGAATTAAAGGGATAAATAACGGCGAAATAGAGTGATCAAATAACATCTGTTTATTagccag > 1:466114/1‑71 (MQ=255) gAAAAGGGAATTAAAGGGATAAATAACGGCGAAATAGAGTGATCAAATAACGTCTGTTTATTagccagc < 1:2748781/69‑1 (MQ=255) gggAATTAAAGGGATAAATAACGGCGAAATAGAGTGATCAAATAACGTCTGTTTATTAGCCAGCCAgcggc > 1:2027805/1‑71 (MQ=255) taaataaCGGCGAAATAGAGTGATCAAATAACGTCTGttt < 1:4048527/40‑1 (MQ=255) taaataaCGGCGAAATAGAGTGATCAAATAACGTCTGttt < 1:30331/40‑1 (MQ=255) taaataaCGGCGAAATAGAGTGATCAAATAACGTCTGttt < 1:1320077/40‑1 (MQ=255) taaataaCGGCGAAATAGAGTGATCAAATAACGTCTGTTTATTAGCCAGCCAGCGGCGtt < 1:4068920/60‑1 (MQ=255) taaataaCGGCGAAATAGAGTGATCAAATAACGTCTGTTTATTAGCCAGCCAGCGGCGtt < 1:1139253/60‑1 (MQ=255) taaataaCGGCGAAATAGAGTGATCAAATAACGTCTGTTTATTAGCCAGCCAGCGGCGTTGGGTTCGTGAt > 1:667391/1‑71 (MQ=255) aaataaCGGCGAAATAGAGTGATCAAATAACGTCTGTTTATTAGCCAGCCAGCGGCGTTGGGTTCGTGAt > 1:3056207/1‑70 (MQ=255) aataaCGGCGAAATAGAGTGATCAAATAACGTCTGTTTATTAGCCAGCCAGCGGCGTTGGGTTCGTGAt > 1:245341/1‑69 (MQ=255) aataaCGGCGAAATAGAGTGATCAAATAACATCTGTTTATTAGCCAGCCAGCGGCGTTGGGTTCGTg > 1:3406808/1‑67 (MQ=255) gCGAAATAGAGTGATCAAATAACGTCTGTTTATTAGCCAGCCAGCGGCGTTGGGTTCGTGa > 1:3597083/1‑61 (MQ=255) gAAATAGAGTGATCAAATAACGTCTGTTTATTAGCCAGCCAGCGGCGTTGGGTTCGTGATTAGcgacgat > 1:2420044/1‑69 (MQ=255) aaaTAGAGTGATCAAATAACGTCTGTTTATTAGCCAGCCAGCGGCGTTGGGTTCGTGATTAGCGAAGaaaa < 1:2056480/71‑1 (MQ=255) gagTGATCAAATAACGTCTGTTTATTAGCCAGCCAGCGGCGTTGGGTTCGTGAt > 1:1257360/1‑54 (MQ=255) gagTGATCAAATAACGTCTGTTTATTAGCCAGCCAGCGGCGTTGGGTTCGTGAt > 1:397613/1‑54 (MQ=255) gagTGATCAAATAACGTCTGTTTATTAGCCAGCCAGCGGCGTTGGGTTCGTGAt > 1:2667619/1‑54 (MQ=255) aTCAAATAACATCTGTTTATTAGCCAGCCAGCGGCGt < 1:3910682/37‑1 (MQ=255) aTCAAATAACATCTGTTTATTAGCCAGCCAGCGGCGt < 1:550882/37‑1 (MQ=255) aTCAAATAACATCTGTTTATTAGCCAGCCAGCGGCGt < 1:254510/37‑1 (MQ=255) cAAATAACGTCTGTTTATTAGCCAGCCAGCGGCGTTGGGTTCGTGATTAGCGAAGAAAAATCATCATAAAt > 1:981739/1‑71 (MQ=255) aaaTAACGTCTGTTTATTAGCCAGCCAGCGGCGTTGGGTTCGTGATTAGCGAAGAAAAATCATCATAAAtt > 1:1755322/1‑71 (MQ=255) aaTAACGTCTGTTTATTAGCCAGCCAGCGGCGTTGGGTTCGTGATTAGCGAAGAAAAATCATCATAAATTg < 1:167303/71‑1 (MQ=255) gTCTGTTTATTAGCCAGCCAGCGGCGTTGGGTTCGTGATTAGCGAAGAAAAATCATCATAAATTGGTGaaa < 1:937795/71‑1 (MQ=255) | CTGTCAGGCATTTTAGAAAAGGGAATTAAAGGGATAAATAACGGCGAAATAGAGTGATCAAATAACGTCTGTTTATTAGCCAGCCAGCGGCGTTGGGTTCGTGATTAGCGAAGAAAAATCATCATAAATTGGTGAAA > W3110S.gb/1249057‑1249193 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |