| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,275,296 | A→G | 12.5% | intergenic (‑120/‑206) | ychN ← / → ychP | conserved hypothetical protein/predicted invasin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,275,296 | 0 | A | G | 12.5% | 77.0 / 5.0 | 32 | intergenic (‑120/‑206) | ychN/ychP | conserved hypothetical protein/predicted invasin |
| Reads supporting (aligned to +/- strand): ref base A (16/12); new base G (2/2); total (18/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
ACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTT > W3110S.gb/1275241‑1275362 | aCCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGt < 1:635997/71‑1 (MQ=255)aCCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGt < 1:2510942/71‑1 (MQ=255) tCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTc > 1:3877469/1‑71 (MQ=255) cGGTCACATTTTTAAGGATTTTCCTGAACGCGCGAGAAAATACGACaaaa > 1:2879008/1‑50 (MQ=255) ggTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCtt > 1:3849790/1‑71 (MQ=255) tttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTAt > 1:1515115/1‑71 (MQ=255) tttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTAt > 1:3914557/1‑71 (MQ=255) aGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCttt < 1:511302/59‑1 (MQ=255) aGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGt < 1:189079/70‑1 (MQ=255) ttCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTc > 1:2808889/1‑50 (MQ=255) ttCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCt > 1:856996/1‑51 (MQ=255) ttCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCt > 1:3399192/1‑51 (MQ=255) ttCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCt > 1:182871/1‑51 (MQ=255) tCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCat < 1:1964772/70‑1 (MQ=255) tCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCat < 1:3481493/70‑1 (MQ=255) tCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCat < 1:3445619/70‑1 (MQ=255) ccTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCatt < 1:3887065/70‑1 (MQ=255) ccTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCatt < 1:1370913/70‑1 (MQ=255) aaaGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTa > 1:4011125/1‑41 (MQ=255) aaaGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTa > 1:3035144/1‑41 (MQ=255) aGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGt < 1:1112240/37‑1 (MQ=255) gcgAGAAAATACGACAAAAGTTGCCAGTAATCGTTAtt > 1:2676659/1‑38 (MQ=255) gcgAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTa > 1:3496119/1‑43 (MQ=255) gcgAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTa > 1:1707994/1‑43 (MQ=255) aaaaTACGACAAAGGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGttt < 1:2254223/50‑1 (MQ=255) aTACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGa < 1:2262439/71‑1 (MQ=255) aTACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGa < 1:1650566/71‑1 (MQ=255) aCGACAAAGGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCatta > 1:1246233/1‑52 (MQ=255) aCGACAAAGGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCatt > 1:419156/1‑51 (MQ=255) aCGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACg > 1:2227762/1‑71 (MQ=255) aCGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACg > 1:1032849/1‑71 (MQ=255) aCGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAACTTACCGACg > 1:1737605/1‑71 (MQ=255) cAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCTACGtttt < 1:2978309/71‑1 (MQ=255) aaGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTAc > 1:1970171/1‑59 (MQ=255) aaGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTAc > 1:1393741/1‑59 (MQ=255) | ACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTT > W3110S.gb/1275241‑1275362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |